SUN Xieping,GUO Yanhong,YU Xin,et al.Effects of Selenomethionine on Anti-oxidant Capacity of Grape Seedlings of Two Cultivars[J].Northern Horticulture,2020,44(11):13-19.[doi:10.11937/bfyy.20194093]
硒代蛋氨酸对两个葡萄幼苗抗氧化能力的影响
- Title:
- Effects of Selenomethionine on Anti-oxidant Capacity of Grape Seedlings of Two Cultivars
- 文献标志码:
- A
- 摘要:
- 以盆栽“阳光玫瑰”和“早夏香”2个品种葡萄幼苗为试材，采用硒代蛋氨酸处理2个品种使基质硒含量在1.0 mg?L-1，研究了硒代蛋氨酸对葡萄幼苗生长和生理参数的影响，以期为富硒葡萄的生产提供参考依据。结果表明：Se-Met处理增加了2个品种的硒含量和比叶重，提高了“阳光玫瑰”叶绿素a、叶绿素b、叶绿素a+b含量，而“早夏香”表现不明显。Se-Met处理增加了“阳光玫瑰”叶片丙二醛含量和抗氧化能力，降低了“早夏香”丙二醛含量和抗氧化能力。Se-Met处理使2个品种叶片可溶性蛋白质含量和游离氨基酸含量显著提高，硒含量与比叶重、叶绿素a、叶绿素a+b、可溶性蛋白质、游离氨基酸和丙二醛含量呈正相关。Se-Met处理提高了“阳光玫瑰”谷胱甘肽过氧化酶活性，降低了“早夏香”的活性，而硒对“阳光玫瑰”谷胱甘肽转移酶活性影响不显著，但是显著降低了“早夏香”的活性。总之，盆栽基质硒在1.0 mg?L-1的处理下，显著提高了葡萄叶片硒含量，在叶片和根系生理参数上看“阳光玫瑰”优于“早夏香”。
- Abstract:
- Taking the two potted cultivars of ‘Sunshine rose’ and ‘Early summer’ as the test materials,the selenomethionine (Se-Met) was used as the selenium source to make the substrate selenium content at 1.0 mg?L-1.The growth and physiological parameters of grape seedlings were studied to provide a theoretical basis for the development of selenium (Se)-enriched grapes.The results showed that the content of Se and specific leaf weight were increased by Se-Met treatment.And the contents of chlorophyll a,chlorophyll b,chlorophyll a+b also increased in cultivar ‘Sunshine rose’,but no significant difference appeared in the ‘Early summer’.Moreover,the contents of malondialdehyde (MDA),and the capacity of anti-oxidative were increased by Se-Met treatment in cultivar ‘Sunshine rose’,but reduced in cultivar ‘Early summer’.The contents of soluble protein and free amino acid of the two cultivars were significantly increased by Se-Met treatment.Se content was positively correlated with specific leaf weight,the contents of chlorophyll a,chlorophyll a+b,soluble protein,free amino acide and MDA.Se-Met treatment increased glutathione peroxidase activity of ‘Sunshine rose’,and decreased the activity of ‘Early summer’.However,Se-Met treatments had no significant effect on glutathione transferase activity of ‘sunshine rose’,but significantly reduced the activity of ‘Early summer’.Overall,Se contents of two cultivars was significantly increased under the treatment of 1.0 Se mg?L-1 potted substrate,and the ‘Sunshine rose’ was better than the ‘Early summer’ in the physiological parameters.
参考文献/References:
[1]安梦鱼.富硒土壤硒形态转化的主要影响因子及对作物吸收的研究[D].福州:福建农林大学,2017.[2]刘建福,张瑾洁,张永康.微量元素硒与人体健康关系研究的新进展[J].吉首大学学报(自然科学版),1997(3):72-79.[3]朱燕云,吴文良,赵桂慎,等.硒在动植物及微生物体中的转化规律研究进展[J].农业资源与环境学报,2018,35(3):189-198.[4]孙协平,王武,罗友进,等.提高作物硒含量研究进展[J].湖南农业科学,2015(7):144-147.[5]史祥宾,刘凤之,王孝娣,等.氨基酸硒叶面肥对梨果实硒含量及品质的影响[J].中国南方果树,2016,45(5):105-107,112.[6]童建川.重庆紫色土区硒分布特征研究[J].西南师范大学学报,2016,41(3):170-175.[7]罗友进,韩国辉,孙协平,等.三峡库区(重庆段)土壤硒分布特征及影响因素[J].土壤,2018,50(1):131-138.[8]刘星明,尹国红,郝浩浩,等.富硒核桃生产技术研究[J].现代园艺,2018(21):12-13.[9]戚霄晨,简在海,张琦,等.叶面施硒对甜樱桃硒和重金属含量及果实品质的影响[J].果树学报,2007,20(11):1-12.[10]张陆阳.中国葡萄种植面积超越法国[J].中外葡萄与葡萄酒,2015(5):64.[11]牛林茹.黄白侧耳的富硒栽培研究[D].太原:山西大学,2014.[12]SMITH A L.Separation of the selenium compounds in seleniferous plant protein hydrolysates by paper partition chromatography[D].South Dakota:South Dakota State College of Agriculture and Mechanical Arts,1949.[13]BLAU M,BENDER M A.Se 75-selenomethionine for visualization of the pancreas by isotope scanning[J].Radiology,1962,78(6):974.[14]杨敏文.化学科技活动一则-分光光度法测定叶片叶绿素和类胡萝卜素含量[J].化学教学,2002(8):44-45.[15]杜明,赵镭,赵广华,等.生物样品中微量硒测定方法的进展[J].理化检验(化学分册),2007(9):797-802.[16]OYAIZU M.Studies on products of browning reactions:Antioxidant activities of products of browning reaction prepared from glucosamine[J].Japanese Journal of Nutrition,1986,44:307-315.[17]李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社,2000.[18]邓小芳,吕臣浩,黄立强,等.喷施时期和硒源对“金桃” 猕猴桃硒吸收累积及主要品质指标的影响[J].果树学报,2018,35(11):1385-1392.[19]邓坤.水稻根系吸收和转运硒代蛋氨酸的机制研究[D].洛阳:河南科技大学,2015.[20]孙协平,罗友进,周广文,等.硒的价态与浓度对香橙幼苗生长和AsA-GSH循环的影响[J].应用生态学报,2017,28(11):3527-3534.[21]张杨杨,焦自高,艾希珍,等.土壤增施蛋氨酸硒对厚皮甜瓜生理特性和品质的影响[J].植物营养与肥料学报,2016,22(2):476-485.[22]隋利,易家宁,王康才,等.不同氮素形态及其配比对盐胁迫下紫苏生理特性的影响[J].生态学杂志,2018,37(11):102-108.[23]李会珍,张志军,刘培培,等.不同基因型紫苏耐盐性比较研究[J].西北植物学报,2011,31(2):338-344.[24]张驰,吴永尧,彭振坤,等.硒对油菜苗期脯氨酸和丙二醛含量的影响[J].湖北农业科学,2003(2):45-47.[25]周玫,陈瑗.谷胱甘肽过氧化物酶[J].生命的化学,1985(4):17-18.[26]王赞,曹红利,岳川,等.茶树CsGPX基因全基因组鉴定和表达分析[J].热带作物学报,2019,40(6):1130-1137.[27]梁潘霞,邢颖,廖青,等.硒对春茶谷胱甘肽过氧化物酶活性及品质的影响[J].西南农业学报,2018,31(12):2559-2562.[28]梁勇,陈月星,赵丽那,等.硒酸钠对不同麦类作物谷胱甘肽过氧化物酶活性的影响[J].西南农业学报,2017,30(7):1511-1515.[29]胡廷章.植物的谷胱甘肽转移酶家族[J].重庆三峡学院学报,2004(5):121-124.
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备注/Memo
第一作者简介：孙协平（1983-），女，博士，讲师，研究方向为果树生理生态。E-mail:xieping444@163.com.责任作者：周先容（1968-），男，硕士，教授，研究方向为植物资源与植物生态学。E-mail:zxrfsy@163.com.基金项目：重庆市教委科学技术研究青年资助项目(KJQN201901425)；重庆市科委基础研究与前沿探索资助项目(cstc2018jcyjAX0618)。收稿日期：2020-01-16