LYU Beibei,ZHANG Guiyun,ZHANG Liping,et al.Effects of Rape Rhizosphere and Biofumigation on the Community of Continuous Cropping Soil Fungi[J].Northern Horticulture,2020,44(10):95-103.[doi:10.11937/bfyy.20194058]
油菜根际及生物熏蒸对连作土壤真菌群落的影响
- Title:
- Effects of Rape Rhizosphere and Biofumigation on the Community of Continuous Cropping Soil Fungi
- Keywords:
- rape; biofumigation; continuous cropping obstacle; soil fungi
- 文献标志码:
- A
- 摘要:
- 以多年连作的西葫芦试验田为平台，设置油菜根际土壤（RRS）、油菜非根际土壤（NRS）及油菜生物熏蒸（BFS）3个处理，采用土壤理化分析、高通量测序技术研究生物熏蒸前后土壤环境因子及真菌群落结构的变化。结果表明：在连作西葫芦试验田种植油菜后，油菜根际及破碎后生物熏蒸能够改善土壤养分，改变土壤真菌群落结构组成，抑制或杀害土壤中部分有害病原菌，在一定程度上有助于防治连作引起的土传病害。3个处理在门分类水平上，共检测到5个菌门，且NRS处理存在分类地位不确定真菌门，子囊菌门为绝对优势菌门；3个处理的真菌群落丰富度和多样性指数大小排序为NRS>BFS>RRS，且仅在RRS和NRS处理间存在显著差异；与NRS相比，RRS及BFS能抑制或杀灭土壤中部分有害真菌属；NRS处理的显著性差异物种高于其它2个处理；聚类分析表明NRS与BFS处理土壤真菌群落结构相似；与NRS相比，RRS及BFS处理显著提高了土壤碱解氮、速效钾、有机质含量，降低了土壤pH；CCA分析表明，土壤碱解氮、速效磷及有机质含量是影响真菌群落最为重要的环境因子。
- Abstract:
- Based on summer squash experimental field with continuous cropping for many years,the objective of this study was to research the changes of soil environmental factors and fungal community structure under different treatments by using soil physical and chemical analysis,high-throughput sequencing technology.Three treatments were designed as follows rape rhizosphere soil(RRS),non-rhizosphere soil(NRS) and biofumigation soil(BFS).The results showed that after planting rape in the continuous cropping summer squash experimental field,RRS and BFS could improve soil nutrients,change the structure and composition of soil fungal community,and inhibit or kill some harmful pathogens in the soil,and to some extent,it was helpful to control soil-borne diseases caused by continuous cropping.At the phylum level,five fungal phyla were identified in three treatments,among which Ascomycete was absolutely dominant,and NRS had a classification status uncertain fungal phylum.The richness and diversity indexes of three treatments were ranked as follows,NRS>BFS>RRS,and there was only significant difference between RRS and NRS.Compared with NRS,RRS and BFS could inhibit or kill some harmful fungi in soil.Biomarkers under NRS were higher than the others.UPGMA analysis showed that the soil fungal community structure of NRS and BFS was similar.Compared with NRS,RRS and BFS significantly increased the contents of soil available nitrogen,available potassium and organic matter,and reduced the soil pH.CCA analysis showed that the contents of soil available nitrogen,available phosphorus and organic matter were the most important environmental factors affecting the fungal communities.
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备注/Memo
第一作者简介：吕贝贝（1993-），女，本科，研究实习员，现主要从事农作物病虫害综合治理和植物营养学等研究工作。E-mail:1240651061@qq.com.责任作者：张丽萍（1964-），女，博士，研究员，现主要从事农作物病虫害综合治理等研究工作。E-mail:lipingzh2006@126.com.基金项目：山西省农科院农业科技创新研究资助项目(YCX2018D2YS09，YGJPY1912)。收稿日期：2020-01-10