ZHANG Dezhen,LI Tingting,DAI Huijie,et al.Effects of Bacillus amyloliquefaciens JF-1 on Disease Control Against Fusarium Wilt and Growth Promoting of Cucumber[J].Northern Horticulture,2020,44(10):16-21.[doi:10.11937/bfyy.20194008]
解淀粉芽孢杆菌JF-1对黄瓜的防病促生作用
- Title:
- Effects of Bacillus amyloliquefaciens JF-1 on Disease Control Against Fusarium Wilt and Growth Promoting of Cucumber
- 文献标志码:
- A
- 摘要:
- 为确定解淀粉芽孢杆菌JF-1对黄瓜枯萎病的防效及对黄瓜的促生作用，采用平板对峙试验检测了解淀粉芽孢杆菌JF-1对多种植物病原真菌的抑菌效果，并通过盆栽试验和发芽试验研究了JF-1对黄瓜枯萎病的防治效果及对黄瓜种子萌发和幼苗生长的影响。结果表明：JF-1对6种供试的植物病原真菌均表现出明显的抑菌效果，平均抑菌率为65.91%，其中对黄瓜枯萎病菌的抑菌率为68.10%；接种JF-1可以明显降低黄瓜枯萎病的发病率和病情指数，防治效果可达到75.15%，相对于多菌灵的防效为46.10%；用稀释500倍的JF-1发酵液处理种子可提高黄瓜种子的活力指数21.75%，增加发芽种子的胚轴长度和全株鲜质量，增幅分别为24.08%和30.77%；施用稀释500倍和1 000倍的JF-1发酵液均可以明显促进黄瓜幼苗的生长，显著提高黄瓜幼苗的壮苗指数，增幅分别为43.48%和39.13%，稀释500倍的JF-1发酵液对于增加黄瓜幼苗的侧根数、最大叶面积和叶片绿色度的效果较好，增加幅度分别达到38.32%、52.12%、13.26%。
- Abstract:
- In order to study the effects of Bacillus amyloliquefaciens JF-1 on disease control against Fusarium wilt and growth promotion on cucumber,the antagonistic activity of JF-1 on various plant pathogenic fungi was detected,the control efficacy of JF-1 to cucumber Fusarium wilt and the effect on cucumber seed germination and seedling growth were studied.The results showed that JF-1 displayed antagonistic activity on 6 strains of plant pathogenic fungi obviously,the average bacteriostatic rate was 65.91%,meanwhile the antagonistic rate against cucumber Fusarium wilt was 68.10%.Inoculating JF-1 could reduce the incidence and disease index of cucumber Fusarium wilt significantly.The control effect was 75.15%,and 46.10% compared with carbendazim.When soaked by diluted fermentation broth of JF-1,the vigor index of cucumber seeds increased by 21.75%.The hypocotyl length and fresh weight of the seedlings increased by 24.08% and 30.77%,respectively.The application of JF-1 fermentation broth diluted 500 times and 1 000 times could promote the growth of cucumber seedlings significantly,and increase the seedling index of cucumber seedlings by 43.48% and 39.13%,respectively.The 500 times diluted fermentation broth of JF-1 showed better effects on increasing amount of lateral roots,maximum leaf area and blade green degree.The rang of increase on the 3 indexes was 38.32%,52.12% and 13.26%,respectively.
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备注/Memo
第一作者简介：张德珍（1980-），女，博士，副教授，研究方向为植物病害生物防治。E-mail：zhen5198@126.com.基金项目：山东省高校设施园艺实验室资助项目（2018YY038）；潍坊科技学院博士基金资助项目（2017BS14）。收稿日期：2020-01-13