[1]曾瑞珍,彭成力,夏晴,等.染色体加倍对红掌组培快繁特性的影响[J].北方园艺,2020,44(14):81-86.[doi:10.11937/bfyy.20193735]
　ZENG Ruizhen,PENG Chengli,XIA Qing,et al.Effects of Chromosome Doubling on Micropropagation Charateristics of Anthurium ‘Pink Champion’[J].Northern Horticulture,2020,44(14):81-86.[doi:10.11937/bfyy.20193735]
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染色体加倍对红掌组培快繁特性的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年14 页码: 81-86 栏目: 出版日期: 2020-07-30
Title:
Effects of Chromosome Doubling on Micropropagation Charateristics of Anthurium ‘Pink Champion’
作者:
曾瑞珍1; 2; 彭成力2; 夏晴3; 王月1; 郭和蓉1; 2; 张志胜1; 2
（1.华南农业大学　林学与风景园林学院，广东 广州 510642；2.华南农业大学 广东省植物分子育种重点实验室，广东 广州 510642；3.广州花卉研究中心，广东 广州 510360）
Author(s):
ZENG Ruizhen1; 2; PENG Chengli2; XIA Qing3; WANG Yue1; GUO Herong1; 2; ZHANG Zhisheng1; 2
(1.College of Forestry and Landscape Architecture，South China Agricultural University，Guangzhou，Guangdong 510642；2.Guangdong Provincial Key Laboratory of Plant Molecular Breeding，South China Agricultural University，Guangzhou，Guangdong 510642；3.Guangzhou Flower Research Center，Guangzhou，Guangdong 510360)
关键词:
红掌; 染色体加倍; 快速繁殖特性
Keywords:
Anthurium andraeanum; chromosome doubling; micropropagation characteristics
DOI:
10.11937/bfyy.20193735
文献标志码:
A
摘要:
以二倍体、三倍体和四倍体‘粉冠军红掌’幼叶为外植体，研究了染色体加倍对红掌组培快繁特性的影响。结果表明：不同倍性红掌的幼叶起始脱分化时间相近，愈伤组织诱导率差异不显著，说明染色体加倍对红掌幼叶脱分化影响不显著；染色体加倍对红掌愈伤组织增殖影响显著，在MS+6-BA 1.0 mg?L-1+NAA 0.2 mg?L-1培养基上，二倍体增殖率最高，四倍体次之，三倍体最低；染色体加倍对红掌愈伤组织芽分化也有显著影响，三倍体的芽分化率显著低于二倍体和四倍体，二倍体、三倍体和四倍体愈伤组织芽分化的适宜外源激素浓度分别为6-BA 0.5 mg?L-1+KT 0.2 mg?L-1、6-BA 0.5 mg?L-1+KT 0.5 mg?L-1和6-BA 1.0 mg?L-1+KT 0.2 mg?L-1；染色体加倍对红掌根分化率和试管苗移栽成活率没有显著影响。上述研究结果表明，染色体加倍对红掌组培快繁效率影响显著，且不同倍性影响不同。
Abstract:
The effect of chromosome doubling on micropropagation characteristics of Anthurium ‘Pink Champion’ was investigated by using of young leaves as explants.The results indicated that the initial dedifferentiation time of diploid，triploid and tetraploid Anthurium ‘Pink Champion’ was similar，and the differences in leaf callus induction rate were not significant，suggested that chromosome doubling had little effect on leaf callus induction.Chromosome doubling significantly affected callus proliferation，on MS+6-BA 1.0 mg?L-1+NAA 0.2 mg?L-1 medium，the callus proliferation rate of diploid was the highest，followed by the tetraploid，the proliferation rate of triploid was the lowest.Chromosome doubling also had significant effect on bud differentiation，the bud differentiation rate of triploid was significantly lower than that of diploid and tetraploid，the suitable exogenous hormone concentrations for bud differentiation of diploid，triploid，tetraploid were 6-BA 0.5 mg?L-1+KT 0.2 mg?L-1，6-BA 0.5 mg?L-1+KT 0.5 mg?L-1 and 6-BA 1.0 mg?L-1+KT 0.2 mg?L-1，respectively.Chromosome doubling had little influence on root differentiation rate and transplanting survival rate.The results obtained demonstrated that chromosome doubling had significant effect on micropropagation efficiency in Anthurium andraeanum，which depended on ploidy level.

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备注/Memo

第一作者简介：曾瑞珍（1971-），女，硕士，副研究员，现主要从事花卉遗传育种等研究工作。E-mail：zengrz@scau.edu.cn.责任作者：张志胜(1965-），男，博士，教授，现主要从事花卉遗传育种等研究工作。E-mail：zszhang@scau.edu.cn.基金项目：广东省科技计划资助项目（2018B020202003）；广州市科技计划资助项目（201803020041）；广州市农业局资助项目（1810593,19100210）；广东省农业农村厅资助项目（2019KJ120）。收稿日期：2019-12-03

更新日期/Last Update: 2020-09-15