ZHU Xiuhong,HAN Xiaoxue,WEN Daoyuan,et al.Influence of Cadmium on the Subcellular Fractions and Chemical Forms of Brassica napus L.[J].Northern Horticulture,2020,44(10):1-9.[doi:10.11937/bfyy.20193670]
镉胁迫对油菜亚细胞镉分布和镉化学形态的影响
- Title:
- Influence of Cadmium on the Subcellular Fractions and Chemical Forms of Brassica napus L.
- Keywords:
- Cd; B.napus L.; subcellular fraction; chemical forms; root morphology; physiological and biochemical characteristic
- 文献标志码:
- A
- 摘要:
- 以油菜为胁迫试材，采用水培的试验方法，设置了4个镉水平（0、1、5、10 mg·L-1）处理，研究了Cd胁迫对油菜根系形态、生理特性、亚细胞Cd分布和化学形态的影响,以期为蔬菜的重金属防治提供参考。结果表明：随着Cd胁迫浓度增加，油菜根系活力、根长、根直径、根表面积和体积均先升高后降低，油菜根部和叶部脯氨酸含量和丙二醛（MDA）含量呈现逐渐升高，过氧化物酶（POD）和过氧化氢酶（CAT）活性呈现逐渐降低，超氧化物歧化酶（SOD）活性呈现先升高后降低趋势；叶绿素含量呈现逐渐减小趋势。油菜富集Cd的比例分配趋势表现为细胞液>细胞器>细胞壁，随着Cd胁迫浓度的增大，油菜各个亚细胞组分Cd含量增加，与1 mg·L-1相比，5 mg·L-1和10 mg·L-1处理下油菜根部和叶部细胞壁和细胞器Cd所占比例增加。油菜各化学形态Cd含量趋势为FNaCl>FHAC>FH2O>Feth>FHCl>Fres，且其含量均随镉胁迫浓度的增大而增加，油菜根部和叶部氯化钠提取态所占比例最高，说明吸附Cd比例较高。
- Abstract:
- Brassica napus L. was used as the stress material and tested by hydroponic culture，four Cd levels (0，1，5，10 mg·L-1) were set.The effects of Cd stress on the morphology，physiological characteristics，subcellular Cd distribution and chemical forms of rapeseed were studied in order to provide reference for prevention of heavy metals in vegetables.The results showed that with the increase of Cd stress concentration，root activity，root length，root diameter，root surface area and volume of rapeseed increased first and then decreased，and proline content and MDA content in roots and leaves of rapeseed increased gradually，the activities of POD and CAT decreased gradually，and the activity of SOD increased first and then decreased，the content of chlorophyll showed a decreasing trend.The trend of Cd distribution in B.napus plant showed cytosol>cell organelle>cell wall.With the increase of Cd stress concentration，the Cd content of various subcellular components of B.napus plant increased.Compared with 1 mg·L-1,the proportion of cell wall and organelle Cd in roots and leaves of rapeseed increased under 5 mg·L-1 and 10 mg·L-1 treatment.The trend of Cd content in the chemical forms of rapeseed was FNaCl>FHAC>FH2O>Feth>FHCl>Fres，and its content increased with the increase of cadmium stress concentration.The highest proportion of sodium chloride extracted from rapeseed roots and leaves showed that the ratio of adsorbed Cd was high.
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备注/Memo
第一作者简介：朱秀红（1966-），女，硕士，教授，硕士生导师，现主要从事污染生态等研究工作。E-mail:zhuxiuhong001@126.com.责任作者：茹广欣（1963-），男，博士，教授，博士生导师，现主要从事林木遗传育种等研究工作。E-mail:ruguangxin@163.com.基金项目：国家林草局生物安全与遗传资源资助项目（KJZXSA2019042)；河南省高等学校重点科研资助项目（13A610949）；教育部国家大学生创新创业资助项目（19JX0501)。收稿日期：2019-12-06