[1]童家赟,李韵璇,黄欢,等.温度、赤霉素和硝酸钾溶液浸种对毛冬青种子萌发的影响[J].北方园艺,2020,44(11):112-116.[doi:10.11937/bfyy.20193056]
　TONG Jiayun,LI Yunxuan,HUANG Huan,et al.Effects of Temperature，Gibberellin Acid(GA3) and KNO3 Solution Presoaking on Seed Germination of Ilex pubescens[J].Northern Horticulture,2020,44(11):112-116.[doi:10.11937/bfyy.20193056]
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温度、赤霉素和硝酸钾溶液浸种对毛冬青种子萌发的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年11 页码: 112-116 栏目: 出版日期: 2020-06-15
Title:
Effects of Temperature，Gibberellin Acid(GA3) and KNO3 Solution Presoaking on Seed Germination of Ilex pubescens
作者:
童家赟; 李韵璇; 黄欢; 林绍东; 黄爱华
（广州中医药大学 中药学院，广东 广州 510006）
Author(s):
TONG Jiayun; LI Yunxuan; HUANG Huan; LIN Shaodong; HUANG Aihua
(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510006)
关键词:
毛冬青; 种子萌发; 温度; 赤霉素; 硝酸钾
Keywords:
Ilex pubescens; seed germination; temperature; GA3; KNO3
DOI:
10.11937/bfyy.20193056
文献标志码:
A
摘要:
以低温湿沙贮藏71 d的野生毛冬青（Ilex pubescens）种子为试验材料，设置3个温度组（20、25、30 ℃），6个浸种溶液组（5、10、20 g?L-1赤霉素(GA3)以及5 g?L-1硝酸钾(KNO3)、5 g?L-1 KNO3加10 g?L-1 GA3、对照组）进行萌发试验，以发芽率、发芽势、发芽指数和活力指数等为评价指标，考察各组毛冬青种子萌发和幼苗生长情况，以期筛选出适宜毛冬青种子萌发的最佳温度条件和浸种溶液浓度。结果表明：3个温度条件下毛冬青种子发芽率无显著差异，20 ℃时种子的发芽势和发芽指数均显著低于25 ℃组；30 ℃组种子的活力指数显著低于25 ℃组。用10 g?L-1GA3溶液浸种能显著促进种子萌发，使发芽势和发芽指数等评价指标显著高于对照组（蒸馏水浸种）；5 g?L-1KNO3溶液浸种24 h对毛冬青种子萌发及其幼苗的生长无明显影响。因此，25 ℃是最适宜毛冬青种子萌发的温度，10 g?L-1GA3是最适宜的浸种溶液浓度。
Abstract:
To seek a proper temperature for Ilex pubescens seeds germination and find an optimal concentration of gibberellin acid(GA3) or potassium nitrate（KNO3） for seeds pretreatment.After stored with wet sand at 4 ℃ for 71 days,seeds were set up in 8 groups and sowed in petri dishes.There were 3 groups for temperature investigation,20 ℃,25 ℃ and 30 ℃.And 5,10,20 g?L-1 GA3,5 g?L-1 KNO3,mixed solution of 5 g?L-1 KNO3 and 10 g?L-1 GA3,and distilled water were used to pre-soak the seeds respectively.Parameters of germination percentage,germination energy,germination index,fresh weight of seedlings,vigor index,lengths of radical and hypocotyl were used to investigate the effects.There was no significant difference of the germination percentages between the groups at three temperatures；germination energy and germination index of seeds at 20 ℃ were significantly lower than those in 25 ℃.Vigor index of seeds at 30 ℃ was significantly lower than those in 25 ℃.After soaking seed with 10 g?L-1GA3,the germination energy and germination index were significantly higher than the control group.Conclusion the optimal germination temperature was 25 ℃,and 10 g?L-1 GA3 was suitable for pretreatment of Ilex pubescens seeds before germination.

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备注/Memo

第一作者简介：童家赟（1979-），男，博士，副教授，研究方向为药用植物保护和繁育及中药鉴定。E-mail：tongjy@gzucm.edu.cn.基金项目：国家自然科学基金资助项目(31401277)；中医药公共卫生服务补助专项“全国中药资源普查项目”资助项目（财社[2017]66号）；广州中医药大学薪火计划资助项目（XH20170107）。收稿日期：2019-11-15

更新日期/Last Update: 2020-08-12