[1]刘洋,覃媚媚,罗印斌,等.灵芝孢子主要活性成分及其功能研究进展[J].北方园艺,2020,44(05):138-146.[doi:10.11937/bfyy.20192708]
　Research Progress on Component and Function of Ganoderma lucidium Spore.Research Progress on Component and Function of Ganoderma lucidium Spore[J].Northern Horticulture,2020,44(05):138-146.[doi:10.11937/bfyy.20192708]

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灵芝孢子主要活性成分及其功能研究进展

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷期数: 2020年05 页码: 138-146 栏目: 出版日期: 2020-03-15

Title:: Research Progress on Component and Function of Ganoderma lucidium Spore

作者:: 刘洋1; 覃媚媚1; 罗印斌1; 马先红1; 周鸿立2; （1.吉林化工学院生物与食品工程学院，吉林吉林 132022；2.吉林化工学院化学与制药工程学院，吉林吉林 132022）

Author(s):: Research Progress on Component and Function of Ganoderma lucidium Spore; (1.College of Biological and Food Engineering,Jilin Institute of Chemical Technology,Jilin,Jilin 132022;Institute of Chemical Technology,Jilin Institute of Chemical Technology,Jilin,Jilin 132022)

关键词:: 灵芝孢子; 成分; 功能; 研究进展

Keywords:: Ganoderma lucidium spore; components; function; research progress

DOI:: 10.11937/bfyy.20192708

文献标志码:: A

摘要:: 以灵芝孢子为试材，结合近10年的研究现状，以期明确灵芝孢子主要活性成分种类及功能机制。结果表明：灵芝孢子为灵芝喷射的生殖细胞，含有多糖、蛋白质、三萜类化合物、脂肪酸、矿物质等活性成分。灵芝孢子具有免疫调节、抗肿瘤、治疗神经系统疾病、保肝护肝等多种功能。灵芝孢子活性成分及功能与产地及培养基种类呈现出一定的相关性。该研究可为关于不同地区、不同品种的灵芝孢子的研究明确其主要活性成分、功能的构效关系及在药品、食品等大健康领域的应用提供参考依据。

Abstract:: With Ganoderma lucidum spores as the test material,the main active components and functional mechanism of Ganoderma lucidum spores were clarified combined with the related research status in recent ten years.The results showed that the spores of Ganoderma lucidum were the germ cells sprayed by Ganoderma lucidum,which contained polysaccharides,proteins,triterpenoids,fatty acids,minerals and other active components.Ganoderma lucidum spores had many functions,such as immune regulation,anti-tumor,treatment of nervous system diseases,liver protection and so on.The active components and functions of Ganoderma lucidum spores were related to the place of origin and the type of culture medium.This study could provide reference for the study of Ganoderma lucidum spores in different regions and varieties,the structure-activity relationship of main active components and functions,and the application in medicine,food and other health fields.

参考文献/References:

[1]冯彩霞.利用高压脉冲电场对灵芝孢子破壁的试验研究[D].太谷:山西农业大学,2016.[2]国家药典委员会.中国药典[S].北京:中国医药科技出版社,2010:174-175.[3]林志彬.灵芝的现代研究[M].2版.北京:北京医科大学出版社,2001.[4]张劲松,贾薇,邢增涛,等.灵芝子实体和菌丝体的提取物及其各纯化组份生物活性的比较[J].菌物学报,2004（23）:85-92.[5]于华峥.灵芝孢子粉胞壁多糖的分离纯化及结构特征研究[D].上海:上海师范大学,2015.[6]杨珣,冯俊明,龚国洪,等.破壁灵芝孢子细胞的微观结构[J].贵阳中医学院学报,2004(4):47-48.[7]陈体强,李开本,徐洁,等.原木灵芝孢子研究(II)一般性状与化学成分[J].食用菌学报,1997(3):22-26.[8]COLEMANLF C.Structure ofspore wall in Ganodenna[J].Botanical Gazette,1927(3):43-60.[9]SHINC C,SEO C S.Formatiom of nonbasioearpous basdiospore of Ganoderma lcidum[J].Korea Journal of Mycology.1988,16(4):230-234.[10]赵阳楠,常继青.苯酚硫酸法和间接碘量法测定灵芝多糖含量比较[J].食用菌,2007(3):58-61.[11]黄晓兰,吴惠勤,黄芳,等.破壁与不破壁灵芝孢子粉多糖的分析[J].中草药,2016,37(6):813-816.[12]周亚杰,王梓懿,冯鹏.灵芝孢子粉多糖研究进展[J].中草药,2018,49(21):5205-5210.[13]赵桂梅,张丽霞,于挺敏,等.灵芝孢子粉水溶性多糖的分离、纯化及结构研究[J].天然产物研究与开发,2005,17(2):182-185.[14]赵桂梅,张丽霞,于挺敏,等.灵芝孢子粉水溶性多糖SGL-III的结构研究[J].中国药学杂志,2006,41(12):902-904.[15]张丽霞.灵芝孢子粉多糖的分离纯化及结构研究[D].长春:东北师范大学,2005.[16]江艳,王浩,吕龙,等.灵芝孢子粉多糖Lzps-1的化学研究及其总多糖的抗肿瘤活性[J].药学学报,2005,40(4):347-350.[17]ZHUL F,YAO Y,AHMA D Z,et al.Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic,antitumor and antioxidant function[J].Process Biochemistry,2019,84:103-111.[18]张芝华,俞苓,刘艳芳,等.不同灵芝孢子粉产品的多糖成分比较[J].食用菌学报,2017,24(4):59-64.[19]李坚.破壁灵芝孢子中多糖的提取、纯化及其抗氧化的研究[D].长沙:湖南农业大学,2010.[20]李国香,戴军,朱松,等.微波辅助法提取赤灵芝孢子多糖及其相对分子质量分布分析[J].食品与发酵工业,2009,35(12):168-172.[21]徐锦堂.中国药用真菌学[M].北京:北京医科大学中国协和医科大学联合出版社,1997.[22]CRD,KNEZ,KNEZHRN〖AKCˇ〗I〖AKCˇ〗M.Antitumour,antimicrobial,antioxidantand antiacetylcholinesterase effect of Ganoderma lucidumterpenoids and polysaccharides:A Review[J].Molecules,2018,23(3),649-670.[23]史先敏.中国栽培灵芝三菇成分的高效液相色谱指纹图谱研究[D].南京:南京农业大学,2008.[24]叶丽云,鲁欣,吴恩典,等.四种灵芝产品中多糖和三萜含量的比较[J].中国食用菌,2016,35(5):34-36.[25]李康,娜锟,王兴亚.灵芝破壁孢子粉总三萜提取工艺优化及体外抗肿瘤作用的研究[J].中国现代应用药学,2017,34(9):1219-1224.[26]黄书铭,杨新林,张自强,等.超声循环提取灵芝中三萜类化合物的研究[J].中草药，2004,35(5):508-510.[27]弓晓峰,谢明勇,陈奕.黑灵芝中三萜及其皂苷类化合物总量的光度测定[J].天然产物研究与开发,2006,18(5):825-829.[28]HSUR C,LINB H,CHENC W.The study of super-critical carbon dioxide extaction for Ganoderma lucidum[J].Industrial &Engineering Chemistry Research,2001,40(20):4478-4481.[29]张洁,段继诚,梁振,等.超临界流体萃取-高效液相色谱离线联用分析灵芝中三萜类化合物[J].分析化学,2006,34(4):447-450.[30]刘晶晶,章晋武,徐培娟.超临界CO2萃取灵芝孢子油中三萜类化合物[J].食品研究与开发,2009,30(9):56-58.[31]齐川.不同来源灵芝主要活性成分变异[D].杭州:浙江农林大学,2012.[32]LIN S Q,WANG S Z,LIU B,et al.Studies on chemical components of wood log cultivated and tube-cultivated Ganoderma lucidom 2 nutritional components spore colypeptidesfat[J].Strait Pharmaceutical Journal,2005,17:88-90.[33]周选围,林娟,李奇璋,等.灵芝蛋白类活性成分的研究进展[J].天然产物研究与开发,2007(5):917-924.[34]于华峥,刘艳芳,周帅,等.灵芝子实体、菌丝体和孢子粉化学成分的比较[J].食品与生物技术学报,2016,35(8):823-827.[35]郭金英,朱优优,刘贵巧,等.不同品种灵芝主要活性成分与营养物质比较分析[J].北方园艺,2017(17):177-180.[36]李晓凤,张华琦,施晓丽,等.长白山野生平盖灵芝蛋白质营养价值分析[J].食品研究与开发,2018,39(16):170-173.[37]ZHOU D,ZHOU F,MA J,et al.Microcapsulation of Ganoderma lucidum spores oil:Evaluation of its fatty acids composition and enhancement of oxidative stability[J].Industrial Crops and Products,2019,131:1-7.[38]王晓琴,赖政炀,黄秀丽,等.麦角甾醇在灵芝孢子油鉴别与质量评价中的应用[J].食品科技,2016,41(9):262-266.[39]郑阳.灵芝孢子粉化学成分的研究[D].延边:延边大学,2017.[40]刘国健.灵芝孢子不同有效部位提取及体内抗肿瘤作用研究[D].广州:广东药科大学,2017.[41]NIE S P,ZHANG H,LI W J,et al.Current development of polysaccharides from Ganoderma:Isolation,structure and bioactivities[J].Bioactive Carbohydrates and Dietary Fibre,2013,1(1):10-20.[42]刘艳荣,黄厚今.灵芝孢子粉功能作用的研究进展[J].中国食物与营养,2016,22(4):65-69.[43]王瑛,金捷,金祖汉,等.灵芝孢子粉增强免疫功能的研究[J].中国现代应用药学,2016,33(5):544-546.[44]吴灿明,王立杰,林敬明,等.灵芝孢子粉破壁方法优化及其免疫调节和抗肿瘤机制研究[J].今日药学,2017,27(5):307-311.[45]GUO L,XIE J H,RUAN Y Y,et al.Characterization and immunostimulatory activity of apolysaccharide from the spores of Ganoderma lucidum[J].International Immunopharmacology,2009,9(10):1175-1182.[46]王浩豪.灵芝孢子粉多糖色谱指纹图谱及其免疫活性谱效关系的研究[D].无锡:江南大学,2012.[47]王卫霄,吕艳茹,姚苗苗,等.灵芝孢子粉抗肿瘤活性的研究进展[J].河北医药,2015,37(1):105-108.[48]ZHUX L,CHENA F,LINZ B.Ganoderma lucidum polysaccharides enhance thefunction of immunological effector cells in immunosuppressed mice[J].Journal of Ethnopharmacology,2007,111(2):219-226.[49]CHEN S J,LIN H H,HUANG W C,et al.LingZhi-8 protein (LZ-8) suppresses the production of pro-inflammatory mediators inmurine microglial BV-2 cells[J].Food and Agricultural Immunology,2017,28(6):1393-1407.[50]ZHANG J,TANG Q,ZHOU C,et al.GLIS a bioactive proteoglycan fraction from Ganoderma lucidum,displays anti-tumour activity by increasing both humoral and cellular immune response[J].Life Sciences,2010,87(19-22):628-637.[51]张伟,曾园山,汪洋,等.灵芝孢子促进大鼠受损伤脊髓运动神经元存活及其轴突再生相关蛋白质组学的初步研究[J].中西医结合学报,2006,4(3):298-302.[52]朴玮,韩枫,徐维盛,等.破壁灵芝孢子粉及灵芝提取物的混合物对改善小鼠睡眠功能的研究[J].食品科技,2015,40(11):172-175.[53]魏怀玲,余凌虹,刘耕陶.赤灵芝孢子粉水溶性提取物（肌生注射液）对小鼠的催眠镇静作用[J].中药药理与临床,2000,16(6):12-14.[54]张天柱,赵婉君,吴国梁,等.灵芝孢子粉抗抑郁作用机制研究[J].时珍国医国药,2015,26(1):16-18.[55]张金波,宋汉君,刘爽,等.灵芝孢子粉对戊四氮活化海马神经细胞caspase-9表达的研究[J].现代生物医学进展,2016,16(10):1850-1853.[56]张金波,王淑秋,张玉萍,等.灵芝孢子粉对戊四氮活化海马神经细胞bad,bcl-xl和p53表达的研究[J].中国优生与遗传杂志,2015,23(9):24-27.[57]李野.灵芝孢子粉对力竭小鼠抗氧化损伤、抗运动性疲劳作用的研究[D].大连:辽宁师范大学,2009.[58]赵燕平,冯彩珠,付昌隆,等.破壁灵芝孢子粉对ConA诱导免疫性肝损伤模型小鼠血清ALT、AST水平及肝组织炎症程度的影响[J].浙江中西医结合杂志,2017,27(9):760-762,831.[59]杨丽丽,邓志钦,黄维渊,等.灵芝孢子粉对抗亚急性酒精性肝损伤的实验研究[J].时珍国医国药,2013,24(3):513-514.[60]MA H T,HSIEH J F,CHEN S T.Anti-diabetic effects of Ganoderma lucidum[J].Phytochemistry,2015,114:109-113．[61]XIAO C,WU Q,ZHANG J,et al.Antidiabetic activity of Ganoderma lucidum polysaccharides F31 down-regulated hepatic glucose regulatory enzymes in diabetic mice[J].Journalof Ethnopharmacology,2017,196(1):47-57.

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备注/Memo

第一作者简介：刘洋(1984-)，男，博士，副教授，现主要从事现代食品检测及农产品开发与利用等研究工作。E-mail:liuyang84@126.com.责任作者：周鸿立(1967-)，女，博士，教授，现主要从事天然产物开发与利用等研究工作。E-mail:zhl67@126.com.基金项目：吉林省教育科学“十三五”规划重点课题资助项目（ZD17102）；2020年度第一批吉林市科技创新发展计划“乡村振兴”科技创新专项资助项目。收稿日期：2019-09-20

更新日期/Last Update: 2020-05-03