[1]闫星蓉,李响,张幸幸,等.文冠果幼苗对低温胁迫的适应性生理响应[J].北方园艺,2020,44(04):73-79.[doi:10.11937/bfyy.20191381]
　YAN Xingrong,LI Xiang,ZHANG Xingxing,et al.Adaptive Physiological Response to Low Temperature Stress of Xanthoceras sorbifolia[J].Northern Horticulture,2020,44(04):73-79.[doi:10.11937/bfyy.20191381]
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文冠果幼苗对低温胁迫的适应性生理响应

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年04 页码: 73-79 栏目: 出版日期: 2020-02-28
Title:
Adaptive Physiological Response to Low Temperature Stress of Xanthoceras sorbifolia
作者:
闫星蓉1; 2; 李响1; 张幸幸1; 张芸香1; 2; 郭晋平1; 2
（１.山西农业大学 林学院，山西 太谷 030801；２．功能油料树种培育与研发山西省重点实验室，山西 太谷 030801）
Author(s):
YAN Xingrong1; 2; LI Xiang1; ZHANG Xingxing1; ZHANG Yunxiang1; 2; GUO Jinping1; 2
(1.College of Forest,Shanxi Agricultural University,Taigu,Shanxi 030801;2.Shanxi Key Laboratory of Northern Functional Oil Tree Cultivation and Research,Taigu,Shanxi 030801)
关键词:
文冠果幼苗; 低温胁迫; 生理指标; 响应机制
Keywords:
Xanthoceras sorbifolia seedling; low temperature stress; physiological index; responding mechanism
DOI:
10.11937/bfyy.20191381
文献标志码:
A
摘要:
以A099文冠果30 d实生苗为试材，采用对比试验的方法，在持续4 ℃低温条件下，研究相对电导率(REC)、丙二醛含量（MDA）、可溶性糖含量、可溶性蛋白质含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性7个生理指标在不同处理时间（0、4、12、24、48 h）的变化趋势，旨在探讨文冠果幼苗在低温胁迫下的生理响应，以期评价文冠果幼苗抗低温胁迫的能力。结果表明：不同持续胁迫时间均对文冠果幼苗外观形态有显著的影响；在持续4 ℃低温胁迫下，文冠果幼苗的REC及MDA含量均在前期呈持续上升，后期呈缓慢下降的趋势；可溶性糖、可溶性蛋白质含量呈现上升趋势，POD、CAT活性呈现先上升后下降的趋势，SOD活性呈先下降后上升的趋势，且多个指标之间存在相关性；主成分分析表明，7个主要生理指标累计贡献率达到90.715%，可以被归纳为2个独立综合指标；根据隶属函数分析法得到文冠果幼苗抵御低温胁迫能力由高到低依次为0 h＞48 h＞24 h＞12 h＞4 h。说明文冠果幼苗尚可通过自身的调节抵御外界低温变化。
Abstract:
Thirty-day-old of seedings of Xanthoceras sorbifolia A099 seedlings were used as test materials.The method of contrast test was used to studied the change tendency of the relative conductivity (REC),malondialdehyde(MDA),soluble sugar content,soluble protein content,superoxide dismutase(SOD) activity,peroxidase(POD) activity,catalase(CAT) activity under the condition of 4 ℃ low temperature and different treatment time (0,4,12,24,48 hours).The aim was to study the explore the physiological responses of Xanthoceras sorbifolia seedlings under the low temperature stress.In the meantime,the resistance of Xanthoceras sorbifolia seedlings to low temperature stress could be evaluated.The results showed that the appearance and morphology of Xanthoceras sorbifolia seedlings were significantly affected under 4 ℃ low temperature stress.The REC and MDA contents of Xanthoceras sorbifolia seedlings increased in the early stage and decreased slowly in the late stage.The contents of soluble sugar and soluble protein increased,while the activities of POD and CAT activity increased at first and then decreased.The activities of SOD showed that the trend of decreasing at first and then rising.There was correlation among many indexes.Based on the principal component analysis,the cumulative contribution rate of 7 main physiological indexes was 90.715%,which could be classified into two independent comprehensive indexes.According to the membership function analysis,the ability of Xanthoceras sorbifolia seedlings to resist low temperature stress was 0 h＞48 hours＞24 hours＞12 hours＞4 hours.The results showed that Xanthoceras sorbifolia seedlings could resist the change of low temperature by self-regulation.

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备注/Memo

第一作者简介：闫星蓉（1994-），女，硕士研究生，研究方向为森林培育学。E-mail：1265859146@qq.com.责任作者：郭晋平（1963-），男，博士，教授，博士生导师，现主要从事森林资源培育与经营管理及森林景观生态教学与科研等工作。E-mail:jinpguo@126.com.基金项目：山西省科技攻关资助项目（20120311015-3）；山西省林业科技创新资助项目（2018LYCX32）。收稿日期：2019-07-13

更新日期/Last Update: 2020-03-30