TAN Wei,ZHAO Qifeng,LI Qingliang.TAN Wei1,2,ZHAO Qifeng2,LI Qingliang3[J].Northern Horticulture,2019,43(17):41-49.[doi:10.11937/bfyy.20190175]
基于果皮SSR分子标记的二十九个红色酿酒葡萄资源的亲缘关系分析
- Title:
- TAN Wei1,2,ZHAO Qifeng2,LI Qingliang3
- Keywords:
- winegrape; SSR markers; genetic diversity
- 文献标志码:
- A
- 摘要:
- 为了有效区分和合理利用酿酒葡萄种质资源,减少酿酒葡萄育种工作中亲本选配的盲目性,利用SSR分子标记技术对29份酿酒葡萄资源进行了遗传多样性分析。结果表明:10对SSR引物共扩增出90个位点,观测杂合度为0.482 8~0.965 5,期望杂合度为0.592 3~0.893 5,Shannon多样性指数为1.185 7~2.227 9,多态性位点100%。UPGMA聚类分析结果表明,在遗传相似系数0.16处,29份材料分成两大组,其中含有28个品种的组Ⅰ中,2个欧亚种‘红汁加美’和‘晚红蜜’为亚组Ⅱ;亚组Ⅰ中6个欧亚种(‘美乐181’‘品丽珠327’‘赤霞珠169’‘蛇龙珠’‘马瑟兰c980’和‘宝石解百纳’)之间具有亲子关系,为一类(季组Ⅱ);季组Ⅰ分A、B组,其中A组包含14个欧亚种,1个毛欧杂种、1个种间杂种、1个山欧杂种,B组包括1个种间杂种和3个欧山杂种。聚类分析结果与系谱图一致。该研究结果为酿酒葡萄种质资源保护提供了有效信息,并且为酿酒葡萄育种的亲本选择提供了理论指导。
- Abstract:
- In order to effectively distinguish and rationally use wine grape germplasm resources,and reduce the blindness of parental selection in winegrape breeding,the genetic diversity of twenty-nine winegrape varieties was analyzed by SSR molecular marker technology.The results showed that 10 pairs of SSR primers amplified 90 loci,the observed heterozygosity,the expected heterozygosity and the Shannon diversity index was 0.482 8-0.965 5,0.592 3-0.893 5,1.185 7-2.227 9,and the percentage of polymorphic loci was 100%.The results of UPGMA cluster analysis showed that the 29 materials were divided into two groups at the genetic similarity coefficient of 0.16.Among the Group I including twenty-eight varieties,‘Gamay Teinturier’ and ‘Saperavi’ were classified into the subgroup II;in the Subgroup I,six Vitis vinifera species (‘Merlot 181’‘Cabernet Franc 327’‘Cabernet Sauvignon 169’‘Cabernet Gernischt’‘Marselan c980’‘Ruby Cabernet’) had parent-child relationship,so they classified into one category (Season Group II).The season Group I could classified into Group A and Group B;the former one contains fourteen Vitis vinifera species,one Vitis quinquangularis Rehd.×Vitis vinifera hybrid,one interspecific hybrid,one Vitis amurensis Rupr.×Vitis vinifera hybrid,while the latter one includes one interspecific hybrid and three hybrids between Vitis vinifera and Vitis amurensis Rupr..The UPGMA dendrogram were basically consistent with the pedigree.The results provide effective information for the protection of wine grape germplasm resources and provide theoretical guidance for the selection of parents for wine grape breeding.
参考文献/References:
[1]任国慧,吴伟民,房经贵,等.我国葡萄国家级种质资源圃的建设现状[J].江西农业学报,2012,24(7):10-13.[2]刘崇怀,帖锋,潘兴,等.无核葡萄品种资源性状的聚类分析[J].果树学报,2006,23(4):531-533.[3]GUSTAVO G,JORGE F,LAURA B,et al.Varietal differentiation of Tannat,Cabernet-Sauvignon and Merlot grapes and wines according to their anthocyanic composition[J].European Food Research and Technology,2007,225(1):111-117.[4]FRAIGE K,PEREIRA-FILHO E R,CARRILHO E,et al.Fingerprinting of anthocyanins from grapes produced in Brazil using HPLC-DAD-MS and exploratory analysis by principal component analysis[J].Food Chemistry,2014,145(4):395-403.[5]刘闯萍,王军.SSR标记及其在葡萄上的应用[J].果树学报,2008,25(1):93-101.[6]陈小宇,刘林德,葛宜和,等.‘蛇龙珠’葡萄新株系遗传多样性的SSR、ISSR和VvmybA1基因片段分析[J].果树学报,2017,4(2):145-156.[7]RIAZ S,NANGL G S,EDWALDS K J,et al.A microsatellite marker based framework linkage map of Vitis vinifera L.[J].Theoretical and Applied Genetics,2004,108(5):864-872.[8]CHEN C,ZHOU P,CHOI Y A,et al.Mining and characterizing microsatellites from citrus ESTs[J].Theoretical and Applied Genetics,2006,112(7):1248-1257.[9]LMAZIO S,LABRA M,GRASSI F,et al.Chloroplast microsatellites to investigate the origin of grapevine[J].Genetic Resources and Crop Evolution,2006,53(5):1003-1011.[10]CIPRIANI G,SPADOTTO A,JURMAN I,et al.The SSR-based molecular profile of 1005 grapevine(Vitis vinifera L.) accessions uncovers new synonymy and parentages,and reveals a large admixture amongst varieties of different geographic origin[J].Theoretical and Applied Genetics,2010,121(8):1569-1585.[11]LAUCOU V,LACOMBE T,DECHESNE F,et al.High throughput analysis of grape genetic diversity as a tool for germplasm collection management[J].Theoretical and Applied Genetics,2011,122(6):1233-1245.[12]EMANUELLI F,LORENZI S,GRZESKOWIAK L,et al.Genetic diversity and population structure assessed by SSR and SNP markers in a large germplasm collection of grape[J].BMC Plant Biology,2013,13(1):39.[13]GONZALEZ M F,MORALES A M,LZQUIERDO P,et al.Genetic characterization of grapevine(Vitis vinifera L.) cultivars from Castilla L Mancha (Spain) using microsatellite markers[J].Vitis,2007,46(3):126.[14]ULANOVSKY S,GOGORCENA Y,TODA F M D,et al.Use of molecular markers in detection of synonymies and homonymies in grapevines (Vitis vinifera L.)[J].Scientia Horticulturae,2002,92(3):241-254.[15]BOWERS J E,MEREDITH C P.The parentage of a classic wine grape,Cabernet Sauvignon[J].Nature Genetics,1997,16(1):84-87.[16]LAUCOU V,BOURSIQUOT J M,LACOMBE T,et al.Parentage of grapevine rootstock ‘Fercal’ finally elucidated[J].Vitis,2008,47(3):163-167.[17]GOTO-YAMAMOTO N,AZUMA A,MITANI N,et al.SSR genotyping of wild grape species and grape cultivars of Vitis vinifera and V.vinifera×V.labrusca[J].Journal of the Japanese Society for Horticultural Science,2013,82:125-130.[18]李慧,罗正荣,张青林.基于SSR和IRAP标记的‘关口葡萄’亲缘关系分析[J].果树学报,2014,31(6):1040-1046.[19]郭春苗,李宁,杨波,等.葡萄种质资源亲缘关系的SSR分析[J].中国农学通报,2015,31(28):93-98.[20]董志刚,刘伟,李晓梅,等.15个酿酒葡萄品种的分子身份证构建[J].中国农业科技导报,2017,19(9):42-50.[21]王雯染,杨哲,杨航宇,等.葡萄砧木品种的SSR分析[J].果树学报,2018,35(1):11-19.[22]王军,贺普超.山葡萄基因组 DNA 提取及RAPD鉴定[J].果树科学,2000,17(2):79-82.[23]李昂,葛颂.植物保护遗传学研究进展[J].生物多样性,2002,10(1):61-71.[24]李慧峰,车根,李林光,等.果树资源遗传多样性研究进展[J].山东农业科学,2009(5):32-35,40.[25]UPADHYAY A,SABOJI M D,REDDY S,et al.AFLP and SSR marker analysis of grape rootstocks in Indian grape germplasm[J].Scientia Horticulturae,2007,112(2):176-183.[26]JAHNKE G,KOCSISN MOLNR G,MJER J,et al.Analysis of grape rootstocks by SSR markers[J].Journal International des Sciences de la vigne et du vin=International Journal of Uine and Wine Sciences,2012,45(4):199-210.[27]SEFC K M,STEINKELLNER H,GLSSL J,et al.Reconstruction of a grapevine pedigree by microsatellite analysis[J].Theoretical and Applied Genetics,1998,97(1/2):227-231.[28]MARTINEZ L E,CAVAGNARO P F,MASUELLI R W,et al.SSR-based assessment of genetic diversity in South American Vitis vinifera varieties[J].Plant Science,2006,170(6):1036-1044.
相似文献/References:
[1]赵竹君,张振文.山东平度爱迪尔酒庄主要酿酒葡萄品种的研究[J].北方园艺,2013,37(13):15.
ZHAO Zhu-jun,ZHANG Zhen-wen.Study on the Major Grape Varieties in Pingdu Ideal Winery[J].Northern Horticulture,2013,37(17):15.
[2]梁曼,王华,叶秋红.四个西班牙酿酒葡萄品种在陕西关中地区的引种表现[J].北方园艺,2014,38(05):39.
LIANG Man,WANG Hua,YE Qiu-hong.Primary Reports on Introduction of Four Spanish Winegrapes in Guanzhong Area of Shaanxi Province[J].Northern Horticulture,2014,38(17):39.
[3]周兴,王振平,代红军.不同施肥处理对“蛇龙珠”葡萄光合性能及品质的影响[J].北方园艺,2013,37(14):1.
ZHOU Xing,WANG Zhen-ping,DAI Hong-jun.Effects of Different Fertilizer Treatments on Photosynthesis and Quality of ‘Cabernet’ Grape[J].Northern Horticulture,2013,37(17):1.
[4]王正义,王玉平.灌溉定额对酿酒葡萄生长和品质的影响[J].北方园艺,2014,38(06):39.
WANG Zheng-yi,WANG Yu-ping.Effect of Irrigation Quota on Growth and Quality of Wine Grape[J].Northern Horticulture,2014,38(17):39.
[5]黄学春,李映龙,单守明,等.调亏灌溉对“蛇龙珠”葡萄果实生长发育和品质的影响[J].北方园艺,2013,37(23):23.
HUANG Xue-chun,LI Ying-long,SHAN Shou-ming,et al.Effects of Regulated Deficit Irrigation on Berry Development and Quality of ‘Cabernet Gernischt’[J].Northern Horticulture,2013,37(17):23.
[6]岳海英,马海军.不同负载量对酿酒葡萄果实品质的影响[J].北方园艺,2013,37(15):44.
YUE Hai-ying,MA Hai-jun.Effect of Different Loads on Fruit Quality of Wine Grape[J].Northern Horticulture,2013,37(17):44.
[7]法洁琼,张振文.甘肃河西走廊产区主栽酿酒葡萄品质比较研究[J].北方园艺,2013,37(03):26.
FA Jie-qiong,ZHANG Zhen-wen.Study on the Quality Comparision of the Wine Grape in Gansu Corridor Area[J].Northern Horticulture,2013,37(17):26.
[8]李欣,张光弟,李玉鼎.简易埋土法在贺兰山东麓酿酒葡萄越冬防寒中的应用研究[J].北方园艺,2013,37(03):34.
LI Xin,ZHANG Guang-di,LI Yu-ding.Application Study on the Way of Easy Soil-burying for Grapewine Cold Resistant at Eastern Foot of Helan Mountain in Winter[J].Northern Horticulture,2013,37(17):34.
[9]梁艳英,刘旭,隋银强,等.几个红色酿酒葡萄品种成熟度研究[J].北方园艺,2013,37(07):1.
LIANG Yan-ying,LIU Xu,SUI Yin-qiang,et al.Study on the Maturity of a Few of Red Vitis Vinifera Varities[J].Northern Horticulture,2013,37(17):1.
[10]李欣,张光弟,李玉鼎.聚苯乙烯颗粒材料覆盖对贺兰山东麓酿酒葡萄越冬防寒的效果[J].北方园艺,2013,37(07):29.
LI Xin,ZHANG Guang-di,LI Yu-ding.Effect of Granular Polystyrene Materal Covering on Cold Resistant for Overwintering of Grapewine at Eastern Foot of Helan Mountain in Winter[J].Northern Horticulture,2013,37(17):29.
备注/Memo
第一作者简介:谭伟(1985-),女,博士,副教授,研究方向为葡萄育种及栽培生理。E-mail:tanweisdau@163.com.责任作者:李庆亮(1985-),男,博士,副教授,现主要从事果树生理及病虫害防治等研究工作。E-mail:liang85h@163.com.基金项目:枣庄学院博士科研启动基金资助项目(1020707);山西省应用基础研究面上青年基金资助项目(201601D202067);山西省农业科学院院优势课题组自选资助项目(YYS1704)。收稿日期:2019-03-12