XUN Zhili,ZHANG Ling,WEN Xiangzhen,et al.Effect of CO2 Supplementation on Nutrient Content in Tomato Leaves at Different Nitrogen Treatments[J].Northern Horticulture,2019,43(13):1-10.[doi:10.11937/bfyy.20183940]
不同氮处理下增施CO2对番茄叶片养分含量的影响
- Title:
- Effect of CO2 Supplementation on Nutrient Content in Tomato Leaves at Different Nitrogen Treatments
- Keywords:
- nitrogen; elevated CO2; the total dry weight; mineral element
- 文献标志码:
- A
- 摘要:
- 为探究不同氮处理对番茄植株叶片养分的影响及增施CO2的效果。以番茄“鸿途”为试验材料，在2个自然光照人工气候室内，采用苗钵基质栽培，设置5个氮素处理的营养液（50、150、250、350、450 mg?L-1，分别设为N1~N5）、2个CO2浓度（300、600 μL?L-1，分别设为C1、C2），分别测定植株开花期、坐果期、果实膨大期生物量及叶片硝态氮和矿质元素含量。结果表明：中氮处理（N 250~350 mg?L-1）下，植株干质量、叶片硝态氮含量以及矿质元素N、P、K、Ca、Mg含量均较高。增施CO2处理后，番茄植株干质量较C1处理增加了10.2%，叶片N、K、Mg含量都增加了20%左右，P含量没有变化，Ca含量降低了25.67%；低氮处理（N 50 mg?L-1）下，番茄植株干质量、叶片硝态氮含量以及矿质元素N、P、K、Mg、Ca含量均较对照N3处理低。增施CO2处理后，植株的总干质量没有显著增加，其叶片硝态氮含量以及N、P、K含量也没有显著变化，而Ca含量在开花期和坐果期较C1处理分别增加了37.72%、15.45%，Mg含量在开花期较C1处理增加了43.86%；高氮处理（N 450 mg?L-1）下，植株干质量较N3处理降低了18.03%，叶片硝态氮含量较N3处理增加了111.44%，叶片N、Ca、Mg含量与N3处理接近均较高，P、K含量较N3处理均降低了10%。增施CO2处理后，植株干质量较C1处理增加了35.92%，叶片硝态氮含量增加不显著，叶片N、K、Mg含量较C1处理分别增加了19.06%、27.82%、24.87%，而叶片P含量变化不显著，叶片钙含量在番茄开花期、坐果期和果实膨大期都较低，较C1处理分别降低了21.37%、17.16%、7.75%。综合番茄生长各项指标及经济效益，在营养液N浓度为250~350 mg?L-1时，增施CO2浓度到600 μL?L-1（C2）最能促进开花期番茄植株叶片养分含量的增加。
- Abstract:
- To explore the effect of different nitrogen treatmenton the nutrient of tomato leaves and the effect of CO2 supplementation.Tomato variety of ‘Hongtu’ was cultured in two artificial climate room under natural light in the pot with substrate.Five nutrient solutions of different nitrogen treatment were adopted(50,150,250,350,450 mg?L-1，respectively was N1-N5),and two CO2 concentrations were setted up(300,600 μL?L-1，respectively were C1,C2).The whole plant biomass，the contents of nitrate nitrogen and mineral elements in leaves were determined at the stage of plant flowering，fruit setting，fruit enlargement，respectively.The results showed that under the medium N level (N 250-350 mg?L-1)，the total dry weight and the contents of nitrate nitrogen and mineral elements N，P，K，Ca and Mg of the leaves were higher.After supplemented CO2，the dry weight of tomato plants was increased by 10.2% compared with the control (C1).The contents of N，K and Mg in the leaves were increased by about 20%，while the content of P was unchanged，and the Ca content was decreased by 25.67%；under low N level (N 50 mg?L-1)，the plant dry weight and the contents of nitrate nitrogen and mineral elements N，P，K，Ca and Mg were lower than those of N3 treatment.After supplemented CO2 treatment，the total dry weight of plant was no significant change，and the contents of the nitrate nitrogen and N，P，K were no significant change，but the contents of Ca at flowering period and fruit setting period were increased by 37.72% and 15.45% compared with the control(C1)，respectively，the content of Mg at flowering period was increased by 43.86% than C1 treatment；under the high N level (N 450 mg?L-1)，the plant dry weight was lower by 18.03% than N3 treatment，nitrate nitrogen content in the leaf was increased by 111.44% than N3 treatment，and the contents of N，Ca，Mg in leaf closed to that of N3 treatment，were both higher，the contents both of P and K reduced by 10% than N3 treatment.After supplemented CO2，the plant dry weight increased by 35.92% than the control，and the content of nitrate nitrogen in the leaves was no significant increase，the contents of N，K，Mg were increased by 19.06%，27.82% and 24.87% than C1 treatment，respectively.However，the P contents in the leaves was no significant change，the Ca contents in the leaves at the stage of tomato flowering period，fruit setting and fruit enlargement period were lower compared with C1 treatment，were reduced by 21.37%，17.16% and 7.75%，respectively.Comprehensive the growth indicators and economic benefit，the nutrient contents oftomato leaves during flowering period were increased，in the condition of nutrient solution N concentration of 250 mg?L-1 (N3)-350 mg?L-1 (N4)，and elevated CO2 concentration to 600 μL?L-1 (C2).
参考文献/References:
[1]李文庆,张民,束怀瑞.氮素在果树上的生理作用[J].山东农业大学学报(自然科学版),2002,33(1):96-100.[2]刘保才,赛富昌,李英敏.影响日光温室蔬菜产量的三大要素分析[J].河南农业科学,1995(11):34-35.[3]LEAKEYA D B,AINSWORTHE A,BERNACCHIH C J,et al.Elevated CO2 effects on plant carbon,nitrogen,and water relations:Six important lesions from FACE[J].Journal of Experimental Botany,2009,60(10):2859-2876.[4]BEHBOUDIAN M H,LAI R.Carbon dioxide enrichment in virosa tomato plant:Responses to enrichment duration and to temperature[J].Horticulture Science,1994,29(12):1456-1459.[5]OOSTEN J,WILKINS D,BESFORD R T.Some relationships between the gas exchange biochemistry and molecular biology of photosynthesis during leaf development of tomato plants after transfer to different carbon dioxide[J].Plant Cell and Environment,1995,18(11):1253-1266.[6]于承艳,都韶婷,邢承华,等.CO2浓度对番茄幼苗生长及养分吸收的影响[J].浙江大学学报(农业与生命科学版),2006,32(3):307-312.[7]TONGBAI P,KOZAI T,OHYAMA K.CO2 and air circulation effects on photosynthesis and transpiration of tomato seedings[J].Scientia Horticulture,2010,126(3):338-344.[8]BAUER G A,BERTSON G M,BAZZAZ F A.Regenerating temperate forests under elevated CO2 and nitrogen deposition:Comparing biochemical and stomatal limitation of photosynthesis[J].New Phytol,2001,152:249-256.[9]LI F S,KANG S Z,ZHANG J H.Interactive effects of elevated CO2,nitrogen and drought on leaf area,stomatal conductance,and evapotranspiration of wheat[J].Agricultural Water Managemet,2004,67:221-233.[10]HYMUS G J,BAKER N R,LONG S P.Growth in elevated CO2 can both increase and decrease photochemistry and photoinhibition of photosyn thesis in a predictable manner[J].Plant Physiology,2001,127:1204-1211.[11]王芳芳.柑橘四种砧木在营养钵栽培下对总和矿质元素的吸收性评价[D].武汉:华中农业大学,2012.[12]LEGG J O,STANFORD G.Utilization of soil and fertilizer N by oats in relation to the available N status of soils[J].Soil Science Society of America Journal,1967,31(2):215.[13]SAENGWILAI P,TIAN X,LYNCH J P.Low crown root number enhances nitrogen acquisition from low-nitrogen soils in maize[J].Plant Physiology,2014,166(2):581-589.[14]LYNCH J P.Steep,cheap and deep:An ideotype to optimize water and N acquisition by maize root systems[J].Annals of Botany,2013,112(2):347-357.[15]石德成,殷立娟.盐(NaCl)与碱(Na2CO3)对星星草胁迫作用的差异[J].植物学报,1993,35(2):144-149.[16]李廷轩,张锡洲,王昌全,等.保护地土壤次生盐渍化的研究进展[J].西南农业学报,2001(S1):103-107.[17]侯文娟.增温和施氮对松嫩草原羊草叶片矿质营养元素的影响[D].长春:东北师范大学,2011.[18]PREGIZER K S,BURTON A J,KING J S,et al.Soil respiration,root biomass,and root turnover following long-term exposure of northern forests to elevated atmospheric CO2 and tropospheric O3[J].New Phytologist,2008,180:153-161.[19]李娟,周建明.CO2与NH+4/NO-3比互作对番茄幼苗培养介质pH、根系生长及根系活力的影响[J].植物营养与肥料学报,2007,13(5):865-878.[20]王红玉,曲英华,周士力,等.CO2增施与养分交互作用对日光温室番茄生长的影响[J].农业机械学报,2014,45(S1):182-188.[21]ROGERS G,MIIHAM P,THIBAUD M,et al.Interactions between rising CO2 concentration and nitrogen supply in corn growth and leaf nitrogen concentration[J].Functional Plant Biology,1996,23:119-125.[22]KIM H,LIEFFERING M,MIURA S,et al.Growth and nitrogen uptake of CO2-enriched rice under field conditions[J].New Phytologist,2001,150:223-229.[23]郭嘉.稻田系统主要矿质元素生物有效性对大气CO2浓度升高的响应研究[D].南京:南京农业大学,2011.[24]门中华,李生秀.CO2浓度对冬小麦氮代谢的影响[J].中国农业科学,2005(2):320-326.[25]LARIOS B,AGUERA E,CABELLO P,et al.The rate of CO2 assimilation controls the expression and activity of glutamine synthetase through sugar formation in sunflower(Helianthus annuus L.) leaves[J].Journal of Experimental Botany,2004,55(394):69-75.[26]庄明浩,李迎春,郭子武,等.CO2浓度升高对毛竹和四季竹叶片主要养分化学计量特征的影响[J].植物营养与肥料学报,2013,19(1):239-245.[27]ROGERS H H,RUNION G B,KRUPA S V.Plant responses to atmospheric CO2 enrichment with emphasis on roots and the rhizosphere[J].Environmental Pollution,1994,83(1-2):155-189.[28]王月.CO2浓度升高对不同供磷番茄根系生长和根系分泌物的影响[D].杭州:浙江大学,2008.[29]HERMANS C,VUYLSTEKE M,COPPENS F,et al.Systems analysis of the responses to long-term magnesium deficiency and restoration in Arabidopsis thaliana[J].New Phytologist,2010,187:132-144.[30]YAMANAKA T,NAKAGAWA Y,MORI K,et al.[STBX]MCA1 [STBZ]and [STBX]MCA2[STBZ] that mediate Ca2+ uptake have distinct and overlapping roles in Arabidopsis[J].Plant Physiology,2010,152:1284-1296.[31]GEBAUER R L E,REYNOLDS J F,STRAIN B R.All ometric relations and growth in Pinus taeda:The effect of elevated CO2,and changing N availability[J].New Phytologist,1996,134(1):85-93.[32]THOMPSON G B,WOODWARD F J.Some influences of CO2 enrichment,nitrogen nutrition and competition on grain yield and quality in spring wheat and barley[J].Exp Bot,1994,45(7):937-942.[33]于佳,于显枫,郭天文,等.施氮和大气CO2浓度升高对春小麦拔节期光合作用的影响[J].麦类作物学报,2010,30(4):651-655.[34]李娟,周健民,段增强,等.养分与CO2交互作用对番茄幼苗生长及一些生理指标的影响[J].西北农业学报,2005(4):10-13.[35]KOGAWARA S,NORISADA M,TANGE T,et al.Elevated atmospheric CO2 concentration alters the effect of phosphate supply on growth of Japanese red pine(Pinus densiflora) seedlings[J].Tree Physiology,2006,26:25-33.[36]LUTZE J L,GIFFORD R M.Nitrogen accumulation and distribution in Danthonia richardsonii swards in response to CO2 and nitrogen supply over four years of growth[J].Global Change Biol,2000(6):1-12.[37]PRITCHARD S,ROGERS H H,PRIOR S A,et al.Elevated CO2 and plant structure:A review[J].Global Change Biology,1999(5):807-837.[38]HICKLENTON P R,JOLIFFE P A.Alteractions in the physiology of CO2 exchange in tomato plants grown in CO2 enriched atmospheres[J].Canadian Journal of Botany,1980,58:2181-2189.[39]YELLE S,BEESON R C,TRUDEL M J,et al.Acclimation of two tomato species to high atmospheric CO2,sugar and starch concentrations[J].Plant Physiol,1989,90:1465-1472.[40]PORTER M A,GRODZINSKI B.Acclimation to high CO2 in bean carbonic anhydrase and ribulose bisphosphate carboxylase[J].Plant Physiology,1984,74(2):413-416.[41]PORTER M A,GRODZINSKI B.Growth of bean in high CO2:Effects on shoot mineral composition[J].Journal of Plant Nutrition,1989,12(2):129-144.[42]李娟,周健民,段增强,等.养分与CO2交互作用对番茄幼苗生长及一些生理指标的影响[J].西北农业学报,2005(4):10-13.
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备注/Memo
第一作者简介：荀志丽（1986-），女，博士研究生，研究方向为蔬菜栽培与生理生态。E-mail：zhili20052@126.com.责任作者：李亚灵（1962-），女，博士，教授，博士生导师，现主要从事蔬菜栽培与生理生态等研究工作。E-mail：yalingli1988@163.com.基金项目：山西省自然科学基金资助项目（201801D121239）；国家自然科学基金重点资助项目（61233006）。收稿日期：2019-02-14