LI Yan,SONG Kaixuan,ZHAO Jing,et al.Effect of Drought Stress Stimulated by PEG on Growth and Anti-oxidative Enzyme Activity in White Clover[J].Northern Horticulture,2019,43(11):92-96.[doi:10.11937/bfyy.20183816]
聚乙二醇（PEG）模拟干旱胁迫对三叶草生长及抗氧化酶活性的影响
- Title:
- Effect of Drought Stress Stimulated by PEG on Growth and Anti-oxidative Enzyme Activity in White Clover
- 文献标志码:
- A
- 摘要:
- 以白三叶草（Trifolium repens L.）为试材，采用不同浓度的聚乙二醇（PEG 6000）模拟干旱条件，研究三叶草幼苗生长及保护酶活性的变化，为白三叶草耐旱生理机制阐明提供依据。结果表明：当PEG浓度达到10%时，植株的干质量和鲜质量达到最高；丙二醛（MDA）含量随干旱程度增加先下降后上升,在PEG浓度为5%时最低；超氧化物歧化酶（SOD）活性在PEG浓度为5%时达到最大值，后又逐渐下降；过氧化物酶（POD）活性在PEG浓度为10%时达到最大值，随后缓慢下降；对照株过氧化氢酶（CAT）活性最高。三叶草具有一定的抗旱能力，低浓度的PEG能够促进三叶草的生长，SOD和POD对于三叶草在轻度干旱条件下的良好生长起到了重要的保护作用。
- Abstract:
- PEG 6000 solution was used to simulate drought stress,the growth and protective enzyme activities of white clover were studied in order to provide the basis for elucidating the physiological mechanism of drought tolerance of white clover.The results indicated that when PEG concentration reached 10%,the dry weight and fresh weight of plants reached the highest level.The content of MDA decreased first and then increased with drought degree,it reached the lowest when PEG concentration was 5%.The SOD activity reached the maximum when PEG concentration was 5%,and then decreased slowly;POD activity reached the maximum when PEG concentration was 10%,then decreased slowly;the CAT activity of the control strain was the highest.White clover had drought resistance,low concentration of PEG could promote white clover.Under mild drought conditions,SOD and POD may be key factors for drought tolerance of white clover.
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备注/Memo
第一作者简介：李妍（1975-），女，硕士，副教授，现主要从事植物生理与分子生物学等研究工作。E-mail:lylxy0524@163.com.基金项目：国家自然科学基金资助项目（21671031）；山东省自然科学基金资助项目（BS2014SW006）；大学生创新训练资助项目（201810448039）。收稿日期：2019-03-13