LI Rungen,ZENG Huilan,RONG Huazhen.Effects of Fusarium proliferatum Treatment on Expression of Protective Enzymes Genes in Edible Lilies[J].Northern Horticulture,2019,43(11):55-60.[doi:10.11937/bfyy.20183539]
层出镰刀菌处理对食用百合保护酶相关基因表达的影响
- Title:
- Effects of Fusarium proliferatum Treatment on Expression of Protective Enzymes Genes in Edible Lilies
- Keywords:
- Fusarium proliferatum; edible lilies; real-time quantitative PCR; protective enzyme-related genes; relative expression level
- 文献标志码:
- A
- 摘要:
- 以4种食用百合为试材，利用实时荧光定量PCR(RT-qPCR)方法，研究了层出镰刀菌处理对食用百合保护酶相关基因(POD、CAT)相对表达量的影响，以期为研究食用百合抵抗逆境的分子机制提供参考依据。结果表明:POD在“龙牙×兰州”（R6-2、6）杂交百合、“铁炮×兰州”杂交百合、“兰州”百合被处理后的第1天相对表达量达到峰值且显著高于处理后的其它时间段，分别约为对照组的18.57、63.81、51.41、37.42倍，然而“铁炮”百合则是被处理后的第3天相对表达量达到峰值且显著高于处理后的其它时间段，约为对照组的113.19倍；CAT在“龙牙×兰州”（6）杂交百合、“铁炮×兰州”杂交百合均是被处理后的第1天相对表达量达到峰值且其显著高于处理后的其它时间段，分别约为对照组的68.02、19.77倍，在“龙牙×兰州”（R6-2）杂交百合、“兰州”百合则是被处理后的第3天相对表达量达到峰值，且显著高于处理后的其它时间段，分别约为对照组的30.69、25.19倍，铁炮百合是被处理后的第5天基因相对表达量达到峰值且显著高于其它时间段，约为对照组的71.83倍。
- Abstract:
- Four edible lilies were used as materials,to explore the defense mechanism of edible lilies after the treatment of Fusarium proliferatum,real-time quantitative PCR (RT-qPCR) was used in this research to study the effect of treatment with Fusarium proliferatum on the relative expression of protective enzyme-related genes (POD,CAT) in edible lily.The results showed that the relative expression of POD got the peak in the first day after the treatment in the ‘Longya×Lanzhou’(R6-2,6) hybrid lily,‘Longiflorum×Lanzhou’ hybrid lily and ‘Lanzhou’ lily and was significantly higher than that of other time periods after treatment,about 18.57 times,63.81 times,51.41 times,37.42 times of the control group,respectively.However,it was in the third day after the treatment that the relative expression of POD in the ‘Longiflorum’ reached the peak and was significantly higher than the other time periods after treatment,about 113.19 times of the control group.The relative expression of CAT in the ‘Longya×Lanzhou’ (6) hybrid lily,‘Longiflorum×Lanzhou’ hybrid lily reached the peak in the first day after treatment and was significantly higher than the other periods after treatment,about the control group 68.02 times and 19.77 times,respectively.The relative expression of CAT in the ‘Longya×Lanzhou’ (R6-2) hybrid lily,‘Lanzhou’ lily reached the peak in the third day after treatment and was significantly higher than the other periods after treatment,about the control group 30.69 times and 25.19 times,respectively.However,the relative expression of CAT in the fifth day after the treatment of the ‘Longiflorum’ reached the peak and was significantly higher than the other time periods after treatment,about 71.83 times of the control group.
参考文献/References:
[1]陈立德.摘花和喷施多效唑对百合鳞茎产量与品质的影响[D].长沙：湖南农业大学,2010.[2]刘峰.保健蔬菜食用百合病害的发生与防治[J].上海蔬菜,2013(3):44-46.[3]丁丁,吕长平,刘飞,等.百合枯萎病研究进展[J].江苏农业科学,2009(3):128-130.[4]边小荣,师桂英,梁巧兰,等.兰州百合枯萎病病原菌的分离鉴定与致病性测定[J].甘肃农业大学学报,2016,51(4):58-64.[5]李润根,程华,张鹏,等.百合枯萎病菌Fusarium proliferatum的鉴定、生物学特性及抑菌药剂筛选[J].基因组学与应用生物学，2018,37(9):3844-3851.[6]杨秀梅,王继华,王丽花,等.百合品种抗病基因同源序列分析及抗枯萎病的鉴定[J].园艺学报，2012,39(12):2404-2412.[7]马璐琳，张艺萍，丁鲲,等．百合抗镰刀菌资源鉴定及抗病相关基因筛选[J]．园艺学报,2012,39(6):1141-1150.[8]王升平,杨金广,战徊旭,等.烟草过氧化氢酶基因CAT1的克隆及表达特征分析[J].中国烟草学报,2014,20(5):103-109.[9]张廷伟，刘长仲．禾谷缢管蚜对三个小麦品种幼苗保护酶的影响[J].植物保护,2014,37(4):72-75.[10]徐雪莲,张银东,符悦冠,等.蚜虫侵害对西瓜保护酶活性的影响[J].北方园艺,2014(1):109-111.[11]宋瑞芳,丁永乐,宫长荣,等.烟草抗病性与防御酶活性间的关系研究进展[J].中国农学通报,2007(5):309-314[12]张梦如,杨玉梅,成蕴秀,等.植物活性氧的产生及其作用和危害[J].西北植物学报,2014，34(9):1916-1926.[13]ＲAO J,LIU D Q,ZHANG N N,et al.Identification of genes differentially expressed in a resistant reaction to Fusarium oxysporum in Lilium regale by SSH[J].IEＲI Procedia,2013,5(5):95-101.[14]马璐琳,崔光芬,王祥宁,等.泸定百合过氧化氢酶(Ls-Cat1)基因的克隆及表达分析[J].核农学报,2017,31(9):1700-1707.[15]杨嫦丽,王有国,王祥宁,等.镰刀菌诱导的泸定百合SSH文库构建及抗病相关基因筛选[J].西北植物学报,2014,34(11):2170-2175.[16]吴元华,文才艺,李浩戈,等.烟草感染马铃薯Y病毒脉坏死株系后六种酶活性变化的研究[J].中国烟草学报,1999(2):33-37.[17]魏颖颖,王凤龙,钱玉梅,等.烟草和黄瓜花叶病毒互作中过氧化氢酶的动态变化[J].植物病理学报,2005(4):359-361.[18]丁丁,吕长平,王继华,等.百合抗尖孢镰刀菌无性系的离体筛选[J].湖南农业大学学报(自然科学版),2011,37(1):34-38.[19]高福元,张吉立,刘振平.冬季低温对4种彩叶植物SOD、POD活性影响的研究[J].中国农学通报,2010,26(5):169-173.[20]李润根,程华.食用百合种质资源对枯萎病抗性的鉴定[J].北方园艺,2017(23)：1-6.
相似文献/References:
[1]高磊,符庆功,邵泱峰,等.南方山地食用百合高效栽培技术[J].北方园艺,2014,38(23):47.
[J].Northern Horticulture,2014,38(11):47.
[2]万珠珠,牛来春,谭秀梅,等.三种食用百合种质资源限制生长保存及遗传稳定性研究[J].北方园艺,2016,40(13):89.[doi:10.11937/bfyy.201613024]
WAN Zhuzhu,NIU Laichun,TAN Xiumei,et al.Study on in vitro Limited Growth Conservation and Genetic Stability of Three Edible Lily[J].Northern Horticulture,2016,40(11):89.[doi:10.11937/bfyy.201613024]
[3]张玉芹,李锡香,王海平.食用百合种质资源离体保存技术研究[J].北方园艺,2011,35(02):0.[doi:10.11937/bfyy.201102052]
ZHANG Yu-qin,LI Xi-xiang,WANG Hai-pin.Study on in vitro Conservation Technique of Edible Lily[J].Northern Horticulture,2011,35(11):0.[doi:10.11937/bfyy.201102052]
[4]李润根,程华.食用百合种质资源对枯萎病抗性的鉴定[J].北方园艺,2017,41(23):1.[doi:10.11937/bfyy.20171749]
LI Rungen,CHENG Hua.Resistance Identification of Edible Lily Germplasm Resources to Fusarium Wilt Disease[J].Northern Horticulture,2017,41(11):1.[doi:10.11937/bfyy.20171749]
备注/Memo
第一作者简介：李润根（1966-），男，江西新余人，硕士，副教授，现主要从事食用百合抗病育种等研究工作。E-mail:13507058200@163.com.基金项目：江西省科技厅重点研发资助项目(20161BBF60040)。收稿日期：2019-01-15