XU Shijuan,LU Haixiao,HUANG Huaxi,et al.Determination of Echinacoside in Introducing Plant of Echinacea purpurea[J].Northern Horticulture,2019,43(09):119-124.[doi:10.11937/bfyy.20183502]
引种紫锥菊中松果菊苷含量的分析测定
- Title:
- Determination of Echinacoside in Introducing Plant of Echinacea purpurea
- 文献标志码:
- A
- 摘要:
- 采集云南省蒙自市引种栽培的紫锥菊，以其根、茎、叶和头状花序为试材，采用高效液相色谱(HPLC)方法研究了松果菊苷的定量分析方法，并对建立的分析技术进行方法学考察，运用该定量分析技术对紫锥菊中不同部位的松果菊苷含量进行测定。结果表明：松果菊苷含量在0.021～0.321 μg范围内与峰面积之间呈良好的线性关系，平均加标回收率为93.24%，RSD值为1.28%；紫锥菊不同部位松果菊苷含量在0.075%~0.221%范围内，且叶、头状花序中松果菊苷含量显著高于根和茎中含量。建立的松果菊苷测定分析方法定性定量准确、可靠、重现性好，可用于紫锥菊药材中松果菊苷的质量评价；云南省蒙自市引种栽培生产的紫锥菊药材中松果菊苷含量较高，优于国外原产地及国内报道的引种栽培区。
- Abstract:
- The test materials were the the root,stem,leaf and head inflorescence of Echinacea purpurea,which was introduced and cultivated in Mengzi city,Yunnan Province.The quantitative analysis method of echinacoside in Echinacea purpurea was studied by using the method of high performance liquid chromatography(HPLC).The quantitative analysis method of HPLC of determining echinacoside in Echinacea purpurea were optimized and investigated.The results showed that there was in a good linear relationship with the peak area in the range of 0.021 to 0.321 μg.The average recovery rate was 93.24%,and the RSD value was 1.28%.The content of echinacoside was in the range of 0.075%-0.221% in different parts of Echinacea purpurea,and the content of echinacoside in leaves and capitulum was significantly higher than that in roots and stems.The determination method with good precision and reproducibility was reliable,and could be used for the quality evaluation of echinacoside in Echinacea purpurea.The content of echinacoside in Echinacea purpurea for cultivated in Mengzi city,Yunnan Province was high,which is superior to foreign origin and domestic reported cultivation areas.
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备注/Memo
第一作者简介：徐世娟(1985-)，女，硕士，助教，研究方向为应用分离分析。E-mail:274535264@qq.com.责任作者：陈荣(1977-)，男，博士，教授，硕士生导师，研究方向为药用植物资源开发与保护。E-mail:282473041@qq.com.基金项目：国家自然科学基金资助项目（31660090)；玉林师范学院2018年度高层次人才科研启动基金资助项目（G2018028）。收稿日期：2018-12-03