LIN Ying,SI Chuncan,HUANG Liping,et al.Isolation and Promoting Characteristics of the Rhizospheric Bacteria of Cinnamomum camphora[J].Northern Horticulture,2019,43(04):59-64.[doi:10.11937/bfyy.20182556]
香樟根际促生菌的筛选与促生特性研究
- Title:
- Isolation and Promoting Characteristics of the Rhizospheric Bacteria of Cinnamomum camphora
- 文献标志码:
- A
- 摘要:
- 以香樟根际土壤为试材，采用PVK平板检测法、亚历山大平板检测法、CAS平板检测法、JNFb培养基检测法、Salkowski比色法，分别研究了从香樟根际土中随机挑取的22株细菌的解磷、解钾、产铁载体、固氮、产吲哚-3-乙酸(IAA)能力，采用16S rDNA对产铁载体和产IAA能力较强的菌株进行了分子鉴定，以期为探讨香樟速生、耐贫瘠等机制，同时也为香樟及其它植物研制微生物菌肥提供一定的参考依据。结果表明：挑取的22株细菌中，有7株具有解磷能力，8株具有固氮能力，11株能产铁载体，16株能产IAA。对初步分离的具有产铁载体和产IAA的菌株进行进一步的定量测定发现，菌株XZ3和XZ22产生的铁载体活性单位明显高于其它菌株，分别为78.4%和74.6%。XZ11和XZ13产IAA的能力最强分别为46.82、46.19 μg?mL-1。经16S rDNA分子鉴定，XZ3和XZ22分别隶属短小芽孢杆菌(Lysinibacillus sp.)和芽孢杆菌（Bacillus sp.),XZ11和XZ13分别隶属假单胞菌（Pseudomonas sp.）和短小芽孢杆菌(Lysinibacillus sp.)。
- Abstract:
- In order to provide some reference value for studying the mechanism of Cinnamomum camphora′s fast growth and good adaptability to infertile soil,meanwhile for development microbial fertilizer for C.camphora and other plants.Taking the rhizosphere soil of C.camphora as test materials,22 strains were random took from C.camphora rhizosphere soil,using PVK solid soluble phosphorus method,Alexander plate assay method,CAS plate assay method,JNFb medium assay method,and Salkowski colorimetric method to respectively to test their phosphate solubilisation activity,potassium solubilisation activity,secreting iron ability,nitrogen fixation ability and producing IAA ability.The strains performed stronger secreting iron ability and producing IAA ability were selected for 16S rDNA molecular identification.The results showed that of the 22 strains isolated,7 strains had phosphate solubilizing ability,8 strains were capable of nitrogen fixation,11 strains were capable of producing iron,and 16 strains could produce IAA.Further quantitative determination of the initial isolated strains with siderophore and IAA,the result was found that the siderphore units of XZ3 (78.4%) and XZ22 (74.6%) were significantly higher than those of other strains.XZ11 and XZ13 were the most capable of producing IAA with 46.82 μg?mL-1 and 46.19 μg?mL-1 respectively.XZ3 and XZ22 belonged to Lysinibacillus sp.and Bacillus sp.,XZ11 and XZ13 belonged to Pseudomonas sp.and Lysinibacillus sp.respectively by the 16Sr DNA.
参考文献/References:
[1]ZHOU Y,YAN W.Conservation and applications of camphor tree (Cinnamomum camphora) in China:Ethnobotany and genetic resources[J].Genetic Resources and Crop Evolution,2016,63(6):1049-1061.[2]He X B,HAN G M,LIN Y H,et al.Diversity and decomposition potential of endophytes in leaves of a Cinnamomum camphora plantation in China[J].Ecological Research 2012,27(2):273-284.[3]GIRI N,FAN Z,KORIMORT T,et al.The impact of Cinnamomum camphora on the dissipation of PAHs in diesel contaminated soils from China[J].Spanish Journal of Soil Science,2014,4(2):192-203.[4]YANG B,LIN S,LIN Y,et al.PAHs uptake and translocation in Cinnamomum camphora leaves from Shanghai,China[J].Science of the Total Environment,2017,574:358-368.[5]HO J R,MA H W,WANG Y C,et al.Extraction of heavy metals from contaminated soil by Cinnamomum camphora[J].Ecotoxicology,2014,23(10):1987-1995.[6]ZHAI Y B,DUI Q Y,JIANG K,et al.Traffic-related heavy metals uptake by wild plants grow along two main highways in Hunan Province,China:Effects of soil factors,accumulation ability,and biological indication potential[J].Environmental Science and Pollution Research,2016,23(13):13368-13377.[7]ARYA B,KOMALA B R,SUMALATHA N T,et al.PGPR induced systemic tolerance in plant[J].Int J Curr Microbiol App Sci,2018(7):453-462.[8]KUMAR A,SINGH V K,TRIPATHI V,et al.Chapter 16-Plant growth-promoting rhizobacteria (PGPR):Perspective in agriculture under biotic and abiotic stress[M]//RAM,SARVAJEET S G,NARENDRA T.Crop Improvement Through Microbial Biotechnology，Elsevier，2018：333-342.[9]HAILE D,MEKBIB F,ASSEFA F.Isolation of phosphate solubilizing bacteria from white lupin (Lupinus albus L.) rhizosphere soils collected from Gojam,Ethiopia[J].Journal Fertil Pestic,2016,7(2):1-6.[10]ZHANG C,KONG F.Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants[J].Applied Soil Ecology,2014,82(7):18-25.[11]ECKERT B,WEBER O B,KIRCHHOF G,et al.Azospirillum doebereinerae sp.nov.,a nitrogen-fixing bacterium associated with the C4-grass Miscanthus[J].International Journal of Systematic and Evolutionary Microbiology,2001,51(1):17-26.[12]ALEXANDER D B,ZUBERER D A.Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria[J].Biology and Fertility of Soils,1991,12(1):39-45.[13]STAJKOVIC O,DELIC D,JOSIC D,et al.Improvement of common bean growth by co-inoculation with Rhizobium and plant growth promoting bacteria[J].Romanian Biotechnol Letter,2011,16(1):5919-5926.[14]HU J,ZHONG W,WEIDNER S,et al.Probiotic Pseudomonas communities enhance plant growth and nutrient assimilation via diversity-mediated ecosystem functioning[J].Soil Biology and Biochemistry,2017,113:122-129.[15]孙萌.铁载体高产菌株的ARTP选育及其铁载体产量提高机理的初步分析[D].无锡:江南大学，2017.[16]BAAKZA A,VALA A K,DAVE B P，et al.A comparative study of siderophore production by fungi from marine and terrestrial habitats[J].Journal of Experimental Marine Biology and Ecology,2004,311(1):1-9.[17]BHARUCHA U,PRAJAPATI V,PATEL K,et al.Catecholate type of siderophore production by Enterobacter sp.UB4 isolated from the rhizopheric soil of alfalfa plant[J] .Journal of Cell and Tissue Research,2011,11(3):2967-2971.[18]RAJKUMAR M,AE N,PRASAD M N V,et al.Potential of siderophore-producing bacteria for improving heavy metal phytoextraction[J].Cell,2010,28(3):142-149.[19]GUPTA G,PARIHAR S S,AHIRWAR N K,et al.Plant growth promoting rhizobacteria (PGPR):Current and future prospects for development of sustainable agriculture[J].Microbial and Biochemical Technology,2015,7(2):96-102.[20]ULLAH A,SUN H,MUNIS M F H,et,al.Phytoremediation of heavy metals assisted by plant growth promoting (PGP) bacteria:A review[J].Environmental and Experimental Botany,2015,117:28-40.[21]AMUTHA M.Judith wright′ spoems-paradigms of ecosophy[J].Asian Journal of English and Linguistics,2013,1(1):28-31.
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备注/Memo
第一作者简介：林英(1985-)，女，博士，讲师，现主要从事环境微生物资源研究与开发等工作。E-mail:jslinying@163.com.基金项目：江西省教育厅科学技术研究资助项目(GJJ151277，GJJ151279)；景德镇市科技资助项目（20151NYZD020）。收稿日期：2018-09-10