[1]张剑霜,喻浩,钟欣,等.冬虫夏草及其发酵菌丝体多糖指纹特征[J].北方园艺,2018,42(24):140-145.[doi:10.11937/bfyy.20181302]
　ZHANG Jianshuang,YU Hao,ZHONG Xin,et al.Fingerprint of Polysaccharides in Ophiocordyceps sinensis and Relative Cultivated Mycelium[J].Northern Horticulture,2018,42(24):140-145.[doi:10.11937/bfyy.20181302]
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冬虫夏草及其发酵菌丝体多糖指纹特征

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年24 页码: 140-145 栏目: 出版日期: 2018-12-25
Title:
Fingerprint of Polysaccharides in Ophiocordyceps sinensis and Relative Cultivated Mycelium
作者:
张剑霜1; 2; 喻浩2; 3; 钟欣2; 刘昕2
（1.贵州师范大学 生命科学学院，贵州 贵阳 550025；2.中山大学 生命科学学院,食品与健康工程研究院，广东 广州 510275；3.贵州师范学院 化学与生命科学学院，贵州 贵阳 550018）
Author(s):
ZHANG Jianshuang1; 2; YU Hao2; 3; ZHONG Xin2; LIU Xin2
(1.School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550025;2.Food and Health Engineering Research Center,School of Life Sciences,Sun Yat-sen University,Guangzhou,Guangdong 510275;3.College of Chemistry and Life Sciences,Guizhou Education University,Guiyang,Guizhou 550018)
关键词:
冬虫夏草; 发酵菌丝体; 多糖; 指纹图谱; 聚类分析
Keywords:
Ophiocordyceps sinensis; cultivated mycelium; polysaccharide; fingerprint; cluster analysis
DOI:
10.11937/bfyy.20181302
文献标志码:
A
摘要:
以冬虫夏草为试材，采用PMP衍生化结合HPLC分析建立冬虫夏草多糖指纹图谱分析方法，研究冬虫夏草与冬虫夏草菌丝体之间质量差异。以三氟乙酸水解冬虫夏草多糖，水解产物用1-苯基-3-甲基-5-吡唑啉酮（PMP）衍生化结合HPLC分析测定冬虫夏草多糖指纹，色谱柱为Agilent Eclipse XDB C18（250 mm×4.6 mm，5 μm），流动相为磷酸盐缓冲液（0.1 mol·L-1，pH 6.7）-乙腈（80∶20，v/v），体积流量1 mL·min-1，进样量20 μL，柱温25 ℃。结果表明：不同产地12批次冬虫夏草中多糖指纹相似度均大于0.992，冬虫夏草与冬虫夏草发酵菌丝体多糖指纹存在较大差异，相似度在0.708~0.974。聚类分析与相似度分析结果基本一致，能够实现冬虫夏草与其发酵菌丝体的区分。建立的冬虫夏草多糖HPLC指纹图谱分析法操作简单、结果可靠，能够准确鉴别冬虫夏草与冬虫夏草菌丝体，可用于冬虫夏草质量的客观评价。
Abstract:
A pre-column derivation coupled HPLC analysis was applied to identify and characterize polysaccharides of Ophiocordyceps sinensis and relative cultivated mycelium from different origins.The polysaccharides extracted from O.sinensis were hydrolyzed partially with trifluoroacetic acid and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP).The monosaccharide composition fingerprint was obtained by HPLC on a Agilent Eclipse XDB C18（250 mm×4.6 mm，5 μm）column with phosphate buffered solution (0.1 mol·L-1,pH 6.7) and acetonitrile (80∶20,v/v) as mobile phase at the flow rate of 1 mL·min-1.The column temperature was at 25 ℃.The results showed that the similarity of 12 batches of O.sinensis was more than 0.992.There were some differences between O.sinensis and cultivated mycelium,with the similarity value range from 0.708 to 0.974.The result of Cluster analysis (CA) largely agreed with the similarity evaluation,which distinguished O.sinensis from cultivated mycelium.The polysaccharides fingerprint by HPLC combined with CA were simple and reliable,which could precisely differentiate O.sinensis from cultivated mycelium and were useful for quality evaluation of O.sinensis.

相似文献/References:

[1]雷 桅,税晓容.冬虫夏草生物系统学研究进展[J].北方园艺,2014,38(14):193.
　LEI Wei,SHUI Xiao-rong.Study Advances on Biosystematic of Ophiocordyceps sinensis [J].Northern Horticulture,2014,38(24):193.
[2]税晓容,陈灿,丁元林,等.冬虫夏草发生发育研究中存在的问题研究[J].北方园艺,2016,40(10):184.[doi:10.11937/bfyy.201610048]
　SHUI Xiaorong,CHEN Can,DING Yuanlin,et al.Study on Problems in the Field of Cordyceps sinensis(Berk.)Sacc Occurrence and Development[J].Northern Horticulture,2016,40(24):184.[doi:10.11937/bfyy.201610048]
[3]王 茂 水,徐 明 辉,黄 丽 华,等.冬虫夏草生物活性的研究进展[J].北方园艺,2009,33(07):0.[doi:10.11937/bfyy.200907050]
　W ANG Mao - shui,XU Ming - hui,HUANG Li - hua,et al.Advances in Bioactivities of Cordyceps sinensis[J].Northern Horticulture,2009,33(24):0.[doi:10.11937/bfyy.200907050]

备注/Memo

第一作者简介：张剑霜（1989-），女，博士，讲师，研究方向为微生物药物资源开发利用与质量评价。E-mail:assassin1986@126.com.责任作者：刘昕（1957-），男，硕士，教授，博士生导师，研究方向为微生物药物工程与生物技术。E-mail:lsslx@mail.sysu.edu.cn.基金项目：国家科技支撑计划课题资助项目(2011BAI13B06);国家自然科学基金资助项目（31702006,31300427）;贵州师范大学博士基金资助项目(11904/0517069)。收稿日期：2018-07-03

更新日期/Last Update: 2019-02-11