AI Kaiqi,SU Hua,ZHOU Haijiang,et al.Effects of Different R∶FR Ratio on Chlorophyll Biosynthesis in Tomato Leaves Under Salt Stress[J].Northern Horticulture,2019,43(01):14-22.[doi:10.11937/bfyy.20181267]
不同红光与远红光对盐胁迫下番茄叶绿素合成的影响
- Title:
- Effects of Different R∶FR Ratio on Chlorophyll Biosynthesis in Tomato Leaves Under Salt Stress
- Keywords:
- tomato; red to far-red light ratio; salt stress; chlorophyll biosynthesis precursors; photosynthesis
- 文献标志码:
- A
- 摘要:
- 以番茄“MoneyMaker”植株为试材,采用营养液水培方法,在盐胁迫下设置不同红光(R)与远红光(FR)处理,T1(R∶FR=7.4)、T2(R∶FR=1.2)、T3(R∶FR=0.8),以正常营养液栽培(R∶FR=7.4)为对照,研究了在盐胁迫条件下不同红光与远红光比值(R∶FR)光环境对番茄生长指标、叶绿素及其前体物质含量和气体交换参数的影响,探讨低R∶FR光环境对盐胁迫下叶绿素合成途径的影响。结果表明:在盐胁迫下,番茄植株的干质量、鲜质量、叶绿素a(Chl a)含量、叶绿素b(Chl b)含量、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和胞间CO2浓度(Ci)均显著下降,叶绿素含量下降导致光合能力下降,进一步造成干物质积累减少;在盐胁迫下,降低番茄植株光环境中的R∶FR值,干物质量、气体交换参数和叶绿素含量均显著上升,且在T3处理下显著高于T2处理。盐胁迫下番茄叶片中的5-氨基乙酰丙酸(ALA)、胆色素原(PBG)和尿卟啉Ⅲ(UroⅢ)的含量显著升高,而原卟啉Ⅸ(ProtoⅨ)、镁原叶琳Ⅸ(Mg-protoⅨ)和原叶绿素酸酯(Pchl)显著降低。低R∶FR处理降低了ALA、PBG、UroⅢ的积累量,增加了ProtoⅨ、Mg-protoⅨ、Pchl的含量,同时降低了Chl a/b的值,T2和T3处理中的差异不明显。表明低R∶FR值光环境通过促进叶绿素合成缓解了番茄受到的盐胁迫,R∶FR值为0.8时效果最佳,盐胁迫下番茄叶绿素合成受阻的起始点在ProtoⅨ的合成过程,低R∶FR值能缓解叶绿素合成受阻碍的程度,R∶FR值为1.2和0.8对叶绿素合成的促进没有显著差异。
- Abstract:
- In this study,three groups different red light(R),far-red light(FR) were set under NaCl stress treatment respectively:T1 (R∶FR=7.4);T2 (R∶FR=1.2);T3 (R∶FR=0.8) was compared with normal nutrient solution culture (R∶FR=7.4),effect of different red and far-red ratios (R∶FR) on the growth paramaters,chlorophyll biosynthesis precursors content and gas exchange parameters of tomato under NaCl stress was studied by hydroponics using ‘MoneyMaker’,to explore the effect of low R∶FR value on chlorophyll biosynthesis pathway under NaCl stress.The results showed that,fresh weight,dry weight,contents of chlorophyll a(Chl a) and chlorophyll b(Chl b),net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(Tr) and intercellular CO2 concentration(Ci) of tomato seedlings decreased significantly under conditions of salinity.The decrease of chlorophyll content resulted in the decrease of photosynthetic capacity,which further resulted in the decrease of dry mass accumulation of tomato.Subjected to a lower R∶FR value,tomato seedlings under conditions of salinity increased fresh weight,dry weight,chlorophyll content and gas exchange parameters significantly,which in T3 treatment significantly higher than T2 treatment 5-aminolevulinic acid(ALA),porphobilinogen(PBG) and uroporphyrin Ⅲ(UroⅢ) contents of tomato leaves increased significantly under conditions of salinity,but protoporphyrin Ⅸ(ProtoⅨ),magnesium protoporphyrin Ⅸ(Mg-ProtoⅨ) and protochlorophyllide(Pchl) contents decreased significantly.The accumulation of ALA,PBG,UroⅢ decreased significantly when tomato leaves were subjected to a lower R∶FR value,but ProtoⅨ,Mg-protoⅨ,Pchl contents increased significantly,meanwhile the value of Chl a/b decreased significantly,the differences of T2 and T3 treatment were not significant.These results suggested that the light environment with low R∶FR value alleviated the NaCl stress of tomato seedling by promoting chlorophyll biosynthesis,the effects were the best when the R∶FR value was 0.8.Under NaCl stress,the starting point blocked the chlorophyll synthesis was in ProtoⅨ synthesis process,while low R∶FR value alleviate the obstacles of chlorophyll biosynthesis.There was no difference on promoting chlorophyll biosynthesis between R∶FR value of 1.2 and 0.8.
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备注/Memo
第一作者简介:艾楷棋(1995-),男,硕士研究生,研究方向为设施园艺。E-mail:m18829351908_2@163.com.责任作者:邹志荣(1956-),男,博士,教授,博士生导师,研究方向为设施园艺。E-mail:zouzhirong2005@hotmail.com.基金项目:广西省科技重大专项资助项目(AA16380048);陕西省科技厅资助项目(K3316217078)。收稿日期:2018-07-03