CAO Lihua,ZHU Juanjuan.Effect of Selenium on Germination Rate and Physiological Property Under Salt Stress in Pakchoi[J].Northern Horticulture,2019,43(02):1-7.[doi:10.11937/bfyy.20181090]
外源硒调控盐胁迫下小白菜种子萌发特性和生理特性研究
- Title:
- Effect of Selenium on Germination Rate and Physiological Property Under Salt Stress in Pakchoi
- Keywords:
- selenium; salt stress; seed; germination rate; physiological property
- 文献标志码:
- A
- 摘要:
- 以“四季小白菜”“京新1号”“鲁抗快菜”小白菜种子为试材，采用水培法，研究了NaCl和Na2SeO3处理对小白菜种子发芽势、发芽率、发芽指数、活力指数、相对盐害率、耐盐指数、苗长以及丙二醛（MDA）含量和超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性的影响，以探讨不同品种小白菜耐盐性差异并揭示硒元素缓解小白菜盐胁迫的作用效果。结果表明：150 mmol·L-1和200 mmol·L-1 NaCl处理中“四季小白菜”种子的相对盐害率为3.33%和30.00%，“京新1号”种子的相对盐害率为-8.22%和27.4%，“鲁抗快菜”种子的相对盐害率为25.34%和56.36%，表明“鲁抗快菜”耐盐性最弱。同时，150 mmol·L-1 NaCl处理和1 mg·L-1 Na2SeO3+150 mmol·L-1 NaCl处理中小白菜种子发芽势、发芽率、发芽指数、活力指数和株高均显著低于对照，但1 mg·L-1 Na2SeO3+150 mmol·L-1 NaCl处理中小白菜种子发芽势、发芽率、发芽指数、活力指数和株高较150 mmol·L-1 NaCl处理分别提高17.37%、5.52%、5.36%、159.87%和143.91%，相对盐害率降低为8.28%，耐盐指数增加到77.07%。此外，与150 mmol·L-1 NaCl处理相比，1 mg·L-1 Na2SeO3+150 mmol·L-1 NaCl处理中小白菜幼苗MDA含量显著降低，POD活性和CAT活性均显著升高，故1 mg·L-1 Na2SeO3能够缓解盐胁迫对小白菜幼苗的毒害。
- Abstract:
- The current study explored salt tolerance difference of different varieties in pakchoi and illustrated effects of exogenous selenium (Na2SeO3) on germination feature and physiological property under salt stress in pakchoi in order to reveal function of selenium remitting salt stress.‘Four seasons pakchoi’‘Jinxin one’ ‘Lukang’ were cultivated in manual climatic box with 70% humidity and 25 ℃.The effects of NaCl and selenium on seed germination potential,germination rate,germination index,vigor index,relative salt-harmed rate,salt-tolerance index,seedling length and MDA content,as well as antioxidant enzymes (SOD,POD and CAT) activities were illustrated.The results showed that seed relative salt-harmed rate was 3.33% and 30.00% for ‘Four seasons pakchoi’,-8.22% and 27.4% for ‘Jinxin one’,25.34% and 56.36% for ‘Lukang’ in 150 mmol·L-1 NaCl and 200 mmol·L-1 NaCl treatments,respectively.Thus,‘Lukang’ had the weakest salt tolerance among selected different varieties in pakchoi.Meanwhile,germination potential,germination rate,germination index,vigor index,salt-tolerance index,and seedling length in 150 mmol·L-1 NaCl treatment and 1 mg·L-1Na2SeO3+150 mmol·L-1 NaCl treatments were significant lower than those in control.However,germination potential,germination rate,germination index,vigor index,salt-tolerance index,and seedling length in 1 mg·L-1 Na2SeO3+100 mmol·L-1 NaCl treatment were increased by 17.37%,5.52%,5.36%,159.87% and 143.91%,respectively,in comparison with those in 150 mmol·L-1 NaCl treatment.Relative salt-harmed rate was decreased to 8.28% and salt tolerance index was increased to 77.07%.In addition,MDA content was decreased significantly in 1 mg·L-1Na2SeO3+150 mmol·L-1 NaCl treatment while POD and CAT activities was increased.Therefore,1 mg·L-1 Na2SeO3 could relieve salt stress in pakchoi.
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备注/Memo
第一作者简介：曹丽华（1982-），硕士，林业工程师，现主要从事植物营养等研究工作。E-mail：lhcao2006@163.com.责任作者：朱娟娟（1981-），女，博士，讲师，现主要从事植物营养学等研究工作。E-mail：zjj0954@163.com.基金项目：国家自然科学基金资助项目（31401305）。收稿日期：2018-08-20