[1]符丹丹,张红梅,孙建瑞,等.中国北方苹果炭疽病病原菌遗传多样性的ISSR分析[J].北方园艺,2018,42(13):16-24.[doi:10.11937/bfyy.20180371]
　FU Dandan,ZHANG Hongmei,SUN Jianrui,et al.Genetic Diversity Analysis of Colletotrichum spp.on Apple in North of China Based on ISSR Markers[J].Northern Horticulture,2018,42(13):16-24.[doi:10.11937/bfyy.20180371]
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中国北方苹果炭疽病病原菌遗传多样性的ISSR分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年13 页码: 16-24 栏目: 出版日期: 2018-05-21
Title:
Genetic Diversity Analysis of Colletotrichum spp.on Apple in North of China Based on ISSR Markers
作者:
符丹丹1; 张红梅1; 孙建瑞1; 赵君峰1; 张荣2; 孙广宇2
1.河南科技大学 食品与生物工程学院，微生物资源开发与利用重点实验室，河南 洛阳 471023；2.西北农林科技大学 植物保护学院，旱区作物逆境生物学国家重点实验室，陕西 杨凌 712100）
Author(s):
FU Dandan1; ZHANG Hongmei1; SUN Jianrui1; ZHAO Junfeng1; ZHANG Rong2; SUN Guangyu2
(1.College of Food & Bioengineering,Henan University of Science and Technology/Key Laboratory of Microbial Resources Exploitation and Utilization,Luoyang,Henan 471023;2.College of Plant Protection,Northwest A & F University/State Key Laboratory of Crop Stress Biology for Arid Areas,Yangling,Shaanxi 712100)
关键词:
苹果炭疽病; 炭疽菌属; ISSR 分子标记; 遗传多样性
Keywords:
apple bitter rot; Colletotrichum; ISSR molecular marker; genetic diversity
DOI:
10.11937/bfyy.20180371
文献标志码:
A
摘要:
以我国北方不同地理来源的67株炭疽菌菌株为试材，采用ISSR分子标记聚类分析的方法，研究了苹果炭疽病病原菌的群体遗传多样性和群体遗传结构，以期为研究苹果炭疽病的流行和防治规律提供参考依据。结果表明：10条引物共扩增出65个位点的多态性条带，多态率100.00%。物种水平的聚类分析结果显示，在遗传相似系数0.76水平时，代表性炭疽菌菌株可区分为7个亚群，Nei′s基因多样性指数（H）均大于0.2水平，Shannon信息指数（I）也均大于0.3水平，证明我国北方苹果炭疽病病原菌具有丰富的遗传多样性。通过对3个地理种群的遗传结构分析发现，遗传分化系数（Gst）显示渤海湾区最高，说明渤海湾区的遗传多样性最为丰富，内部的遗传分化较大，但每代迁移数Nm<1，说明种群内的基因交流受到抑制；而后2个地区黄河故道区和西北高原区的Gst值均较小，但种群的每代迁移数Nm较大，说明这2个种群遗传分化较小，种群遗传变异主要来自于群落内，种群内都存在丰富的基因交流。综合3个地理种群每代迁移数Nm=0.72，说明3个地区之间存在一定的菌源交流。
Abstract:
To discuss the genetic diversity of pathogens associated with apple bitter rot at the molecular level,ISSR markers were applied to analyze the genetic diversity of 67 strains of Colletotrichum spp.causing bitter rot of apple in order to supply references for studying the epidemiology and control of apple bitter rot.The results showed that a total of 65 polymorphic bands were amplified with 10 screened primers,and the polymorphic loci percentage was 100.00%.Clustering analysis based on ISSR markers revealed that tested strains were clustered into seven groups at 0.76 genetic similarity coefficient by UPGMA.The results of genetic diversity of 67 strains from different geographical groups showed that Nei′s gene diversity index (H) and Shannon′s information index (I) were respectively greater than 0.2 and 0.3,which indicated that the genetic diversity all over China was considerably abundant.The results of population genetic structure of three geographical groups showed that Gst of the population from Bohai gulf area was the highest,indicating that the genetic diversity of this population was the most abundant and genetic differentiation among groups was bigger.The number of migrants per generation (Nm) of this group was less than 1,indicating that gene flow among groups was restrained.The Gst of the other population were lower but Nm were higher,indicating that genetic differentiation were lower and genetic variations existed within groups.There was significant gene flow within groups of old course of Yellow River and Northwestern Loess Plateau.The total Nm was 0.72,indicating that there was pathogen exchange among three geographical groups.

备注/Memo

第一作者简介：符丹丹（1979-），女，博士，讲师，研究方向为真菌分类学。E-mail:fudandanly@126.com.责任作者：孙广宇（1963-），男，博士，教授，博士生导师，研究方向为真菌分子系统学及生物多样性。E-mail:sgy@nwsuaf.edu.cn.基金项目：国家自然科学基金资助项目 (31171797)。收稿日期：2018-03-21

更新日期/Last Update: 2018-07-24