HENG Jing,HAN Chunye,WANG Mingshan,et al.Induction of Callus of Stem Segments of Lonicera japonica Thunb.var.chinensis (Wats.) Bak[J].Northern Horticulture,2018,42(19):94-99.[doi:10.11937/bfyy.20174342]
红金银花茎段愈伤组织的诱导
- Title:
- Induction of Callus of Stem Segments of Lonicera japonica Thunb.var.chinensis (Wats.) Bak
- 文献标志码:
- A
- 摘要:
- 以红金银花当年生幼嫩茎段为试材，采用正交实验设计，研究了红金银花愈伤组织诱导和增殖培养的影响因素。结果表明:6-BA、KT促进了愈伤组织的诱导，提高了出愈率；当NAA、KT分别为0.4、1.0 mg·L-1时最有利于愈伤组织的增殖培养。不同季节外植体愈伤组织诱导效果不同，春季采集的当年生的幼嫩茎段更有利于愈伤组织的诱导；MS培养基中添加6.5 mg·L-1 琼脂比较适宜；红金银花愈伤组织诱导最适宜的培养基为MS+KT 1.0 mg·L-1+NAA 0.2 mg·L-1+6-BA 1.5 mg·L-1；愈伤组织增殖培养最适宜的培养基为MS+6-BA 1.5 mg·L-1+NAA 0.4 mg·L-1+KT 0.1 mg·L-1。
- Abstract:
- The stem of Lonicera japonica Thunb.var.chinensis (Wats.) Bak was taken as experimental materials,the factors affecting the callus induction and proliferation of Lonicera japonica Thunb.var.chinensis (Wats.) Bak were studied by orthogonal design.The results showed that 6-BA and KT had obviously promoted callus induction and increased the rate of callus induction.It was the most beneficial to the proliferation of callus when the concentration NAA and KT were 0.4 mg·L-1 and 1.0 mg·L-1,respectively.The effect of callus induction was different in different seasons,and the stem segments collected in spring were more favorable for callus induction,and it was better to use 6.5 mg·L-1 agar.The callus induction medium was suitable for MS+KT 1.0 mg·L-1+NAA 0.2 mg·L-1+6-BA 1.5 mg·L-1.The optimum medium for callus proliferation was MS+6-BA 1.5 mg·L-1+NAA 0.4 mg·L-1+KT 0.1 mg·L-1.
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备注/Memo
第一作者简介：衡静（1984-），女，硕士，讲师，研究方向为园林植物栽培及养护。E-mail：hnacwms@126.com．责任作者：马丽（1982-），女，博士，副教授，研究方向为园艺植物抗性生理。E-mail：ndmali@163.com．基金项目：河南省科技攻关资助项目（162102110092）；国家自然科学基金资助项目（31401918）。收稿日期：2018-02-07