PANG Shihua,SHEN Yanhui,YANG Yuanguang,et al.Method Optimization of Determing of Soil Available Phosphorus With Continuous Flow Analytical System[J].Northern Horticulture,2018,42(07):94-98.[doi:10.11937/bfyy.20173182]
连续流动分析仪测定土壤有效磷方法的优化
- Title:
- Method Optimization of Determing of Soil Available Phosphorus With Continuous Flow Analytical System
- Keywords:
- continuous flow analyzer (CFA); molybdenum antimony colorimetric method; optimize; definition
- 文献标志码:
- A
- 摘要:
- 以酸性、中性和碱性土壤为研究对象，采用连续流动分析仪测定土壤有效磷含量，研究了在减少表面活性剂以及提高检测效率的条件下，连续流动分析仪测定方法对土壤有效磷浸提液测定准确性的影响。结果表明：优化后的测定结果与传统紫外分光光度法测定结果相比无明显差异；测定结果之间呈显著线性相关，回归直线方程为Y（连续流动分析仪-P）=1.103 5X（紫外分光光度计-P）-6.439 7，相关系数r=0.999（n=61，P<0.01）；连续流动分析仪测定加标回收率在99.47%~107.40%，重复性测试相对偏差小于1.2%。综上所述，减少表面活性剂以及提高检测效率后，连续流动分析仪测定土壤有效磷检测速度更快，成本更低，重复性更好，能够满足检测要求。
- Abstract:
- Acidic, neutral and alkaline soil were used as research object and tested available P content by continuous flow analyzer（CFA）under the condition of reducing surfactant and increasing detecting efficiency, and evaluated the method accuracy to test leaching liquor of available P.The results showed that there was no significant difference of the soil available P contents measured by CFA and ultraviolet spectrophotometry method.A linear equation could best describe their relationship,Y (CFA-P)=1.103 5X (ultraviolet spectrophotometer-P)-6.439 7.The pearson′s correlation coefficient was 0.999 with a significance level (n=61,P<0.01).The CFA method for soil available P measurement had high precision with relative standard deviation (RSD) less than 1.2%.In summary, after reducing surfactant and improving detection efficiency, continuous flow analyzer could detect soil available phosphorus faster, lower cost, better repeatability, and could meet the requirements of detection.
参考文献/References:
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备注/Memo
第一作者简介：庞世花（1975-），女，山东临沂人，本科，工程师，研究方向为化验分析。E-mail：pangshihua@kingenta.com.责任作者：陈宏坤（1970-），男，博士，研究员，现主要从事新型肥料研发等研究工作。E-mail：chenhk@kingenta.com.基金项目：国家重点研发计划资助项目（2016YFD0222405-7）。收稿日期：2017-09-29