SUN Mingmao,LIU Lixia.Correlation Analysis on Leaf Chlorophyll Content, Malondialdehyde Content and Alkali Damage Index of Lily Seedling Under Alkali Stress[J].Northern Horticulture,2018,42(05):93-98.[doi:10.11937/bfyy.20172582]
百合苗期碱胁迫下叶片叶绿素含量、丙二醛含量和碱害指数相关性分析
- Title:
- Correlation Analysis on Leaf Chlorophyll Content, Malondialdehyde Content and Alkali Damage Index of Lily Seedling Under Alkali Stress
- Keywords:
- field growing seedling of lily; chlorophyll content; malondialdehyde content; alkali damage index; correlation analysis
- 文献标志码:
- A
- 摘要:
- 以百合品种“卷丹”“金百合”“正直”“大黄蜂”大田生长苗为试材，采用0、20、25、30、35 g?L-1 Na2CO3胁迫处理的方法，研究了碱胁迫对百合苗期叶片叶绿素含量、丙二醛含量和碱害指数的影响，以期阐释碱胁迫条件下百合苗期叶片叶绿素a含量、叶绿素b含量、叶绿素总含量、丙二醛含量和碱害指数变异及其相关性。结果表明：随着Na2CO3处理浓度升高，4个百合品种叶片叶绿素a含量、叶绿素b含量和叶绿素总含量平均值均呈下降趋势，而叶片丙二醛含量和碱害指数平均值则呈上升趋势；百合叶片各叶绿素含量指标间存在较多显著正相关和极显著正相关；百合叶片丙二醛含量指标之间、叶片碱害指数指标之间以及叶片丙二醛含量与碱害指数之间存在较多显著和极显著正相关性，而百合叶片叶绿素含量与丙二醛含量之间、叶绿素含量与碱害指数之间相关性不显著。
- Abstract:
- The field growing seedlings of lily varieties ‘Honesty’‘Suncrest’‘L.lancifolium’ and ‘L.trompeten’ were sampled and treated with 0,20,25,30,35 g?L-1 Na2CO3 aqueous solution,respectively.Moreover,variation and correlation analyses on leaf chlorophyll a content,chlorophyll b content,total chlorophyll content,malondialdehyde content and alkali damage index of treated lily seedlings were conducted.With increasing treatment concentration of Na2CO3,the means of leaf chlorophyll a content,chlorophyll b content,total chlorophyll content of four lily varieties showed a downtrend,while the means of leaf malondialdehyde content and alkali damage index showed an uptrend.There were significant positive correlations （P<0.05 or P<0.01) between the leaf chlorophyll contents of lily.Between the leaf malondialdehyde contents,between the leaf alkali damage indexes,between leaf malondialdehyde contents and alkali damage indexes displayed significant positive correlations （P<0.05 or P<0.01).However,the correlations between leaf chlorophyll contents and malondialdehyde contents,between leaf chlorophyll contents and alkali damage indexes were not significant.
参考文献/References:
[1]李晓燕,宋占午,董志贤.植物的盐胁迫生理[J].西北师范大学学报（自然科学版),2004,40（3):106-111. [2]刘杰.盐碱胁迫对向日葵体内矿质营养的影响[J].北方园艺,2014（2):1-5. [3]殷立娟,石德成.东北碱化草地的主要盐分Na2CO3对羊草危害因素分析[J].草业学报,1993,2（1):1-5. [4]华智锐,马锋旺,李小玲,等.百合组培苗对盐胁迫的生理反应[J].西北农林科技大学学报（自然科学版),2007,35（4):179-184. [5]魏祎,张克,王树栋,等.盐胁迫对4个百合品种生理特性的影响[J].北京农学院学报,2010,25（2):52-56. [6]周叶玲,张延龙,牛立新,等.秦巴山区野生百合组培苗耐盐性的初步评价[J].西北农业学报,2011,20（8):147-151. [7]刘坤.岷江百合（Lilium regale Wils.)悬浮细胞系的建立及耐盐筛选[D].南京:南京林业大学,2008. [8]张宪政.植物叶绿素含量测定-丙酮乙醇混合液法[J].辽宁农业科学,1986（3):26-28. [9]王学奎.植物生理生化实验原理和技术[M].2版.北京:高等教育出版社,2006:280-281. [10]喻晓丽.土壤水分胁迫对火炬树幼苗生长和生理生态特征的影响[D].哈尔滨:东北林业大学,2007. [11]李雅男,袁素霞,徐雷锋,等.百合在NaHCO3胁迫下的生理响应机制[J].园艺学报,2016,43（6):1126-1140. [12]陈秋明,尹慧,李晓艳,等.高温胁迫下外源水杨酸对百合抗氧化系统的影响[J].中国农业大学学报,2008,13（2):44-48. [13]戴玉池,邓霞玲,姜孝成,等.不同水稻品种幼苗期的耐寒生理鉴定及其利用[J].湖南师范大学自然科学学报,2004,27（3):86-89. [14]祁栋灵,张三元,槽桂兰,等.水稻发芽期和幼苗前期耐碱性的鉴定方法研究[J].植物遗传资源学报,2006,7（1):74-80.
相似文献/References:
[1]王 薇,宋廷宇,王 艳,等.番茄叶片SPAD值与叶绿素含量的相关性分析[J].北方园艺,2013,37(23):12.
WANG Wei,SONG Ting-yu,WANG Yan,et al.Correlation Analysis on Chlorophyll Content and SPAD Value in Tomato Leaves[J].Northern Horticulture,2013,37(05):12.
[2]刘芳,李怡然,刘峄,等.遮荫对海姆维斯蒂栒子生长及光合特性的影响[J].北方园艺,2014,38(07):52.
LIU Fang,LI Yi-ran,LIU Yi,et al.Effect of Shading on Growth and Photosynthetic Characteristics of Cotoneaster hjelmqvistii[J].Northern Horticulture,2014,38(05):52.
[3]张忠宝,李大成,荆 昕.不同遮荫度和不同栽植密度对独角莲产量的影响[J].北方园艺,2014,38(03):152.
ZHANG Zhong-bao,LI Da-cheng,JING Xin.Effect of Different Shading and Cultivation Density on Yield of Typhonium giganteum [J].Northern Horticulture,2014,38(05):152.
[4]蹇黎,余丹凤,秦小军.野生白芨叶绿素含量与SPAD值的测定与分析[J].北方园艺,2013,37(21):165.
JIAN Li,YU Dan-feng,QIN Xiao-jun.Comparative Analysis on the Chlorophyll Contents and SPAD Value of Wild Bletilla striata Germplasm Resources[J].Northern Horticulture,2013,37(05):165.
[5]潘静,曹兵,万仲武.两种果树叶片SPAD值与叶绿素含量相关性分析[J].北方园艺,2012,36(05):9.
PAN Jing,CAO Bing,WAN Zhong-wu.Relationship Between SPAD Value and Chlorophyll Content in Leaves of Two Fruit Tree Species[J].Northern Horticulture,2012,36(05):9.
[6]王春枝,于小静,齐宝利,等.施肥对南果梨叶片叶绿素含量及光合特性的影响[J].北方园艺,2012,36(14):150.
WANG Chun-zhi,YU Xiao-jing,QI Bao-li,et al.Effect of Fertilize on Chlorophyll Contents and Photosynthetic Characteristics of ‘Nanguo’ Pear[J].Northern Horticulture,2012,36(05):150.
[7]刘伟,丁长春,常征.干旱胁迫下吊石苣苔三种生理指标变化规律研究[J].北方园艺,2014,38(19):65.
LIU Wei,DING Chang-chun,CHANG Zheng.Study on Three Physiological Indexes of Lysionotus pauiflorus Under Drought Stress[J].Northern Horticulture,2014,38(05):65.
[8]杜保伟,方庆,胡月华,等.叶面喷钙对‘3-18’桃叶片性状的影响[J].北方园艺,2014,38(24):21.
DU Bao-wei,FANG Qing,HU Yue-hua,et al.Effect of Spraying CaCl2 on Characters of Peach Strain ‘3-18’ Leaves[J].Northern Horticulture,2014,38(05):21.
[9]马帅,冯金朝,任蓝天,等.葡萄品种“红地球”光合作用对短期不同梯度水分处理的响应[J].北方园艺,2015,39(14):11.[doi:10.11937/bfyy.201514003]
MA Shuai,FENG Jinchao,REN Lantian,et al.The Reaction of Grape ‘Red Global’ Photosynthesis Rate on Short Term Different Level Irrigation Treatments[J].Northern Horticulture,2015,39(05):11.[doi:10.11937/bfyy.201514003]
[10]钟娟,钟俊荣.水分胁迫对金桔光合特性的影响[J].北方园艺,2015,39(06):26.[doi:10.11937/bfyy.201506007]
ZHONG Juan,ZHONG Jun-rong.Effect of Water Stress on Photosynthetic Characteristics of Fortunella margarita[J].Northern Horticulture,2015,39(05):26.[doi:10.11937/bfyy.201506007]
备注/Memo
第一作者简介：孙明茂（1979-），男，山东寿光人，博士，副教授，现主要从事百合种质耐碱性鉴定和育种等研究工作。E-mail：smm-7015@163.com. 基金项目：潍坊科技学院博士基金资助项目（W13K011）；潍坊市科技发展计划资助项目（2014GX049）。收稿日期：2017-10-13