LI Ai,LI Lin,SUN Wangliang,et al.Effects of Exogenous Melatonin on Seed Germination of Perilla frutescens Under Drought Stress[J].Northern Horticulture,2018,42(05):140-144.[doi:10.11937/bfyy.20172549]
外源褪黑素对干旱胁迫下紫苏种子萌发的影响
- Title:
- Effects of Exogenous Melatonin on Seed Germination of Perilla frutescens Under Drought Stress
- Keywords:
- Perilla frutescens; seed germination; melatonin; drought stress
- 文献标志码:
- A
- 摘要:
- 以紫苏（Perilla frutescens(L.) Britton）为试材，利用聚乙二醇（PEG-6000）模拟干旱胁迫环境，分析不同浓度外源褪黑素对紫苏种子萌发特征（发芽率、发芽势、发芽指数和活力指数）和幼苗生长（根长、子叶长、子叶宽和鲜质量）的影响。结果表明：干旱胁迫下紫苏种子萌发受到显著抑制，经过不同浓度的外源褪黑素处理后，发芽指标明显上升，其中处理T1（10%聚乙二醇+50 μmol?L-1褪黑素）各项指标均达到最大〖JP+1〗值，发芽率为64.00%，发芽势为43.67%，发芽指数为17.62，活力指数为0.065。外施褪黑素同样可以缓解干旱胁迫下紫苏幼苗的生长，其中处理T3（10%聚乙二醇+300 μmol?L-1褪黑素）效果最好，幼苗根长为7.45 mm，子叶鲜质量为3.53 mg，长宽比为1.260。表明外施一定浓度的褪黑素能有效缓解干旱胁迫对紫苏种子萌发的影响，为干旱环境下紫苏种植栽培提供了参考依据。
- Abstract:
- In the present study，polyethylene glycol(PEG-6000) was used to simulate the environment of drought stress.The effects of different concentrations of melatonin on seed germination(germination rate，germination potential，germination index，vigor index) and seedlings growth(length of root，length and width of cotyledon，fresh weight of cotyledon) of Perilla frutescens were studied.The results showed that germination of P.frutescens seeds was significantly inhibited under drought stress.However，after treatment at different concentrations of melatonin，every germination index was significantly improved.And every index reached to maximum in T1 treatment(10% polyethylene glycol+50 μmol?L-1 melatonin).The germination rate was 64.00%，the germination potential was 43.67%，the germination index and vigor index were 17.62 and 0.065，respectively.Melatonin treatment also improved the growth of P.frutescens seedlings under drought stress.The effect was most obvious in T3 treatment(10% polyethylene glycol+300 μmol?L-1 melatonin)，and the length of root was 7.45 mm，the fresh weight of cotyledons was 3.53 mg and the length-width ratio of cotyledons was 1.260.All the results showed that application of exogenous melatonin could effectively promote seed germination under drought stress and provided reference for the breeding and cultivation of P.frutescens.
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备注/Memo
第一作者简介：李爱（1984-），女，博士，讲师，研究方向为植物逆境生理与分子生物学。E-mail:lovelee@tjau.edu.cn. 基金项目：天津农学院科学研究发展基金计划资助项目（2016NYB01）；天津市应用基础与前沿技术研究计划资助项目（14JCQNJC15000）；国家自然科学基金青年资助项目（31300564）；天津市“131”创新型人才培养工程资助项目。收稿日期：2017-10-12