[1]姜喜,张琦,赵书珍,等.“矮丰”扁桃休眠芽玻璃化超低温保存条件优化[J].北方园艺,2018,42(01):62-66.[doi:10.11937/bfyy.20172108]
　JIANG Xi,ZHANG Qi,ZHAO Shuzhen,et al.Cryopreservation of Dormant Buds by Vitrification inAlmond Cultivars ‘Aifeng’[J].Northern Horticulture,2018,42(01):62-66.[doi:10.11937/bfyy.20172108]
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“矮丰”扁桃休眠芽玻璃化超低温保存条件优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年01 页码: 62-66 栏目: 出版日期: 2018-01-10
Title:
Cryopreservation of Dormant Buds by Vitrification inAlmond Cultivars ‘Aifeng’
作者:
姜喜1; 2; 张琦1; 赵书珍1; 焦培培2; 庞新安2; 陈家力1
1.塔里木大学 植物科学学院,新疆 阿拉尔 843300;2.新疆生产建设兵团
塔里木盆地生物资源保护利用重点实验室,新疆 阿拉尔 843300
Author(s):
JIANG Xi1; 2; ZHANG Qi1; ZHAO Shuzhen1; JIAO Peipei2; PANG Xin′an2; CHEN Jiali1
1.College of Plant Science，Tarim University，Alar，Xinjiang 843300；2.Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Xinjiang Production & Construction Corps，Alar，Xinjiang 843300
关键词:
“矮丰”扁桃; 休眠芽; 超低温保存; 玻璃化
Keywords:
almond cultivars ‘Aifeng’; dormant bud; cryopreservation; vitrification
DOI:
10.11937/bfyy.20172108
文献标志码:
A
摘要:
以扁桃休眠芽为试材，采用玻璃化超低温保存的方法，研究了休眠芽大小、蔗糖预处理浓度/时间、装载处理时间、PVS2脱水处理时间对扁桃休眠芽超低温保存后的影响，以期为扁桃种质资源的保存提供参考依据。结果表明:从健康的扁桃植株上剥取0.6～1.5 mm（含有1~2个叶原基)休眠芽茎尖，放入含MS的0.5 mol?L-1蔗糖预处理液中预培养1 d后，室温下用装载液（含2 mol?L-1甘油和0.4 mol?L-1蔗糖)装载20 min，0 ℃下用PVS2处理70 min，加入少量新鲜的PVS2后迅速投入液氮罐，保存15 d后取出，在40 ℃水浴锅中化冻2～3 min，用1.2 mol?L-1蔗糖+MS的洗涤液卸载30 min，无菌纸吸干洗涤液后转至恢复培养基中，在24 ℃条件下暗培养 7 d后进行正常光照培养，2周后成活率可达61.01%。
Abstract:
In order to provide a theoretical basis for the preservation of germplasm resources，the effects of sucrose pretreatment time，pretreatment time，loading time and PVS2 dehydration treatment time on the preservation of almond dormant buds were studied by using the method of vitrification and cryopreservation.The results showed that the dormant buds were removed from healthy plants，and the dormant buds were stripped to 0.6-1.5 mm （containing 1-2 leaf primordia) and cultured in MS containing 0.5 mol?L-1 sucrose pretreatment solution 1 day,at room temperature with the liquid loadingof dormant buds loaded 20 minutes，dehydrated 70 minutes under the condition of 0 ℃.Then added a small amount of fresh PVS2，quickly put into liquid nitrogen tank，saved the 15 days out，the dormant buds were thawed 2-3 minutes in a 40 ℃ celsius water bath，uninstalled 30 minutes using 1.2 mol?L-1 sucrose washing solution containing MS medium，after the aseptic paper sucked the washing liquid，it went to the recovery medium.Under the condition of 24 ℃ celsius，the dormant buds were cultured in dark 7 days，and then cultured in normal light.After 2 weeks，the survival rate was 61.01%.

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备注/Memo

第一作者简介：姜喜（1981-),女，湖南宁远人，硕士,高级实验师，研究方向为果树种质资源保存及果树栽培生理。E-mail:1531956730@qq.com.

责任作者：陈家力（1979-)，男，黑龙江宁安人，硕士，副教授，研究方向为果树生理生态及遗传育种学。E-mail：99234902@qq.com.
基金项目：新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放资助项目（BRYB1504)。
收稿日期：2017-07-18

更新日期/Last Update: 2018-01-12