WANG Lei,SUN Yao,LI Yao,et al.Function of eIF-5A Gene in Apocynum venetum L．[J].Northern Horticulture,2017,41(23):190-193.[doi:10.11937/bfyy.20172087]
罗布麻eIF-5A基因功能
- Title:
- Function of eIF-5A Gene in Apocynum venetum L．
- 关键词:
- 罗布麻; 真核细胞蛋白翻译起始因子; 烟草; 抗逆性
- Keywords:
- Apocynum venetum; eIF-5A; tobacco; stress resistance
- 文献标志码:
- A
- 摘要:
- 以罗布麻为试材，采用农杆菌介导法将罗布麻eIF-5A基因导入烟草，分别对正常生长条件和NaHCO3胁迫下转AveIF-5A基因烟草和非转基因烟草的过氧化氢酶(POD)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量进行测定，以期为罗布麻eIF-5A基因功能研究提供参考。结果表明：正常生长条件下，转AveIF-5A基因烟草和非转基因烟草POD活性和MDA含量差异不显著，转基因植株SOD活性显著高于非转基因植株；在NaHCO3胁迫下，转AveIF-5A基因烟草POD活性和SOD活性均极显著高于非转基因植株，而MDA含量极显著低于非转基因植株。AveIF-5A基因过量表达可以提高转基因烟草的耐盐能力。
- Abstract:
- To understand the effect of eIF-5A gene in response to salt stress,the eIF-5A cDNA fragment containing an ORF from Apocynum venetum was transformed into tobacco by Agrobacterium-mediated method.The POD activity,SOD activity and MDA content were measured between transgenic AveIF-5A tobaccos and wild type under normal condition and NaHCO3 stress.The results showed that there was no significant difference in POD activity and MDA content between transgenic and non-transgenic plants under normal condition but SOD activity of transgenic plants increased significantly.Under salt stress,transgenic tobacco over-expressing AveIF-5A gene showed an increase in POD and SOD activity,compared to wild lines.In contrast,transgenic tobacco displayed a decrease in MDA content under NaHCO3 stress.Overall,these results indicated that AveIF-5A gene played a critical role in salt tolerance in transgenic tobacco.
参考文献/References:
[1]张卫明,肖正春,顾龚平,等.罗布麻资源利用与罗布麻植物分类问题[J].中国野生植物资源,2006,25(2):15-19.
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备注/Memo
第一作者简介：王雷（1980-），女，博士，副研究员，研究方向为植物抗逆生理和分子生物学。E-mail:wleileiyu@163.com.