[1]金玉,任梦楠,杨红澎,等.短柄龙胆内生菌的分离鉴定及抗菌活性分析[J].北方园艺,2018,42(01):135-139.[doi:10.11937/bfyy.20171784]
　JIN Yu,REN Mengnan,YANG Hongpeng,et al.Isolation,Identification and Antibiotic Activities of Endophytes in Gentiana stipitata[J].Northern Horticulture,2018,42(01):135-139.[doi:10.11937/bfyy.20171784]
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短柄龙胆内生菌的分离鉴定及抗菌活性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年01 页码: 135-139 栏目: 出版日期: 2018-01-10
Title:
Isolation,Identification and Antibiotic Activities of Endophytes in Gentiana stipitata
作者:
金玉1; 任梦楠1; 杨红澎1; 韦卓1; 确生2; 黄海东1
1.天津农学院 农学与资源环境学院，天津 300384；2.青海师范大学 民族师范学院，青海 西宁 810008
Author(s):
JIN Yu1; REN Mengnan1; YANG Hongpeng1; WEI Zhuo1; QUE Sheng2; HUANG Haidong1
1.College of Agronomy & Resources and Environment,Tianjin Agricultural University,Tianjin 300384;2.Normal College for Nationalities,Qinghai Normal University,Xining,Qinghai 810008
关键词:
内生菌; 短柄龙胆; 类芽孢杆菌; 抗菌活性
Keywords:
endophyte; Gentiana stipitata; Paenibacillus; antibiotic activities
DOI:
10.11937/bfyy.20171784
文献标志码:
A
摘要:
以在青海省西宁市湟中县拉脊山采集的短柄龙胆（Gentiana stipitata）为试材，采用组织块分离和梯度稀释法得到一株具有抗菌活性的内生菌，分析了该内生菌的活性物质，及与龙胆属植物活性成分的关联性，以期为药用植物内生菌资源的开发利用奠定基础。结果表明：内生菌WZ-6对金黄色葡萄球菌、黄瓜褐斑病菌有明显的抑制作用，通过形态学和16S rDNA系统进化分析鉴定，WZ-6为类芽孢杆菌属（Paenibacillus)，与Paenibacillus terrae的同源性最高，达到98.9%，HPLC等分析表明菌株WZ-6的发酵产物中含有环烯醚萜类抑菌活性成分。
Abstract:
Taking Gentiana stipitata as material.An endophytic bacterium with antibacterial activity was isolated by the method of tissue block separation and gradient dilution.Active substance of endophyte and relevance between its and active stubstance of Gentiang stipitata were analyzed,to provided foundation for the development and utilization of endophytes resources from medicinal plants.The results showed that endophytes WZ-6 had significant inhibitory effect on Staphylococcus aureus and Corynespora cassiicola.According to the characteristics of morphology and 16S rDNA phylogenetic analysis,strain WZ-6 was identified as species of genus Paenibacillus,and showed the highest similarity to Paenibacillus terrae,reaching 98.9%.The results of HPLC analysis showed that the fermented product of strain WZ-6 contained antimicrobial active component of iridoid.

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备注/Memo

第一作者简介：金玉（1992-），女，硕士研究生，研究方向为微生物。E-mail：541043612@qq.com.

责任作者：黄海东（1972-），男，博士，教授，现主要从事资源细菌及工程等研究工作。E-mail：hhaidong@126.com.
基金项目：国家自然科学基金资助项目（31571790）。
收稿日期：2017-07-12

更新日期/Last Update: 2018-01-12