[1]赵爽,苏彦苹,李寒,等.核桃花粉萌发适宜培养基的筛选[J].北方园艺,2017,41(23):42-47.[doi:10.11937/bfyy.20171760]
　ZHAO Shuang,SU Yanping,LI Han,et al.Screening of Optimum Medium for Pollen Germination of Walnut[J].Northern Horticulture,2017,41(23):42-47.[doi:10.11937/bfyy.20171760]
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核桃花粉萌发适宜培养基的筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年23 页码: 42-47 栏目: 出版日期: 2017-12-10
Title:
Screening of Optimum Medium for Pollen Germination of Walnut
作者:
赵爽1; 苏彦苹2; 李寒1; 3; 张雪梅1; 3; 范舒畅1; 齐国辉1; 3
1.河北农业大学 林学院，河北 保定 071000;2.廊坊师范学院 生命科学学院，河北 廊坊 065000；3.河北省核桃工程技术研究中心，河北 临城 054300
Author(s):
ZHAO Shuang1; SU Yanping2; LI Han1; 3; ZHANG Xuemei1; 3; FAN Shuchang1; QI Guohui1; 3
1.College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071000；2.College of Life Science，Langfang Teachers University,Langfang,Hebei 065000;3.Research Center for Walnut Engineering and Technology of Hebei,Lincheng,Hebei 054300
关键词:
核桃; 花粉; 培养基
Keywords:
walnut; pollen; medium
DOI:
10.11937/bfyy.20171760
文献标志码:
A
摘要:
以早实核桃品种“绿岭”和“绿波”花粉为试材，采用离体培养方法，研究了培养基中不同比例蔗糖、硼酸、氯化钙对花粉萌发的影响，以明确核桃花粉萌发的适宜条件，为核桃花粉萌发找到适宜的培养基提供参考依据。结果表明：“绿岭”和“绿波”核桃花粉在蔗糖浓度为160 g·L-1时的发芽率最高，分别为48.09%和57.23%；“绿岭”和“绿波”花粉萌发最适宜的硼酸浓度分别为0.10、0.07 g·L-1；2个品种的花粉萌发最适宜的氯化钙浓度为0.20 g·L-1。适宜“绿岭”核桃花粉萌发的最佳培养基为蔗糖160 g·L-1、硼酸0.10 g·L-1、氯化钙0.20 g·L-1，在此培养基下“绿岭”核桃花粉萌发率达到61.06%；适宜“绿波”核桃花粉萌发的最佳培养基为蔗糖160 g·L-1、硼酸0.07 g·L-1、氯化钙0.20 g·L-1，在此培养基下“绿波”核桃花粉萌发率为72.71％。
Abstract:
Pollens of precocious walnut varieties ‘Lyuling’ and ‘Lyubo’ were used by culture method in vitro to study the effects of different proportion of sucrose,in order to determine the appropriate condition for walnut pollen germination and find out optimum medium,boric acid,calcium chloride on pollen germination.The results showed that the pollen germination rates of ‘Lyuling’ and ‘Lyubo’ on the medium with sucrose concentration of 160 g·L-1 were the highest,which were 57.23% and 48.09%,respectively.The optimum boric acid concentrations for pollen germination of ‘Lyuling’ and ‘Lyubo’ were 0.10 g·L-1 and 0.07 g?L-1;the optimum calcium chloride concentration for pollen germination of the two varieties was 0.20 g·L-1;the optimum medium for ‘Lyuling’ walnut pollen germination was as follows,160 g·L-1 sucrose,0.10 g·L-1 boric acid,0.20 g?L-1 calcium chloride, and the pollen germination rate of ‘Lyuling’ walnut on this medium reached 61.06%;the optimum medium for ‘Lyubo’ walnut pollen germination was as follows,160 g?L-1 sucrose,0.07 g?L-1 boric acid,0.20 g?L-1 calcium chloride,and the pollen germination rate of ‘Lyubo’ walnut on this medium reached 72.71%.

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备注/Memo

第一作者简介：赵爽（1990-），女，博士研究生，研究方向为经济林栽培生理。E-mail:zhaoshuang0823@sina.com.

责任作者：齐国辉（1969-），女，博士，教授，现主要从事经济林栽培教学及科研等工作。E-mail:bdqgh@sina.com.
基金项目：“十二五”国家科技支撑计划资助项目（2013BAD14B03）；河北省科技支撑计划资助项目(16236810D)。
收稿日期：2017-07-14

更新日期/Last Update: 2017-12-19