LI Xianliang,ZHAO Zhichang.Optimization of Protocol of Tissue Culture and Rapid Propagation of Pinellia ternata of Protocorm-like Body[J].Northern Horticulture,2018,42(01):14-18.[doi:10.11937/bfyy.20171032]
荆半夏类原球茎组培快繁体系的优化
- Title:
- Optimization of Protocol of Tissue Culture and Rapid Propagation of Pinellia ternata of Protocorm-like Body
- 文献标志码:
- A
- 摘要:
- 以荆半夏为试材,采用正交实验设计,研究了不同外植体及消毒方法对内生菌的影响,并系统地研究了外植体及细胞分裂素对类原球茎诱导的影响、类原球茎向种茎转化的方法,以期为提升半夏类原球茎途径组培快繁效率提供参考。结果表明:叶片未展开时叶柄作外植体,内生菌污染较少,诱导类原球茎效果好。外植体用升汞浸泡前用0.1%苯扎溴铵溶液浸泡3 min有助于降低内生菌污染率。幼嫩的叶柄在培养基MS+TDZ 0.5~1.0 mg?L-1上能较好地形成类原球茎。类原球茎在组培过程中形成根和叶柄后移栽较好,类原球茎在培养基1/3MS+6-BA 0.5 mg?L-1 +NAA 0.1 mg?L-1上能较好形成丛生苗,丛生苗移栽后可形成小块茎。
- Abstract:
- Jing Pinellia ternata was used as test material,in way of orthogonal test method,the influence of the explants and their sterilization methods on contamination from endophyte was studied.And on this base the influence of the explants and the cytokinin on the induction of protocorm-like body and the method of the transformation from protocorm-like body to seed stems were further studied systematically to provide references for rapid progagation of Pinellia ternata seed stems.The results showed that using the young petoiles with unopened leaves as explants and adding the step of using benzalkonium bromide with the concentration of 0.1% soak the explants for 3 minutes could reduce the contamination rate.The young petoiles inculated on the medium of MS+TDZ 0.5-1.0 mg?L-1 could be better induced into protocorm-like bodies.The protocorm-like bodies inoculated on the medium of 1/3MS+6-BA 0.5 mg?L-1 +NAA 0.1 mg?L-1 could form more clustered plantlets,and then more seed stems.
参考文献/References:
[1]楼之岑,秦波.常用中药材品种整理和质量研究[M].北京:北京医科大学中国协和医科大学联合出版社,1995:919.
相似文献/References:
[1]施隆文,汪霞,卢佳.“益培灵”的抑菌效果及对大花蕙兰类原球茎增殖与分化的影响[J].北方园艺,2012,36(04):123.
SHI Long-wen,WANG Xia,LU Jia.The Bacteriostatic Effect of ‘Yipeiling’ and Its Influence on Multiplication and Differentiation of Protocorm Like-body of Cymbidium hybridum[J].Northern Horticulture,2012,36(01):123.
[2]付双彬,池梦薇,杨燕萍,等.虎头兰类原球茎诱导、增殖及保存[J].北方园艺,2020,44(10):71.[doi:10.11937/bfyy.20192474]
FU Shuangbin,CHI Mengwei,YANG Yanping,et al.Induction,Propagation and Conservation of Protocorm-like Bodys of Cymbidium hookerianum[J].Northern Horticulture,2020,44(01):71.[doi:10.11937/bfyy.20192474]
备注/Memo
第一作者简介:李先良(1976-),男,博士,副教授,研究方向为园艺植物遗传改良和分子生物学。E-mail:libusher@sina.com.