LI Ling,WANG Tianmiao,SUN Molong.Extraction and Antioxidant Activity of Alkaloid From Green Peel of Juglans mandshurica Maxim.[J].Northern Horticulture,2017,41(10):126-130.[doi:10.11937/bfyy.201710029]
核桃楸外果皮总生物碱的提取及抗氧化活性的测定
- Title:
- Extraction and Antioxidant Activity of Alkaloid From Green Peel of Juglans mandshurica Maxim.
- Keywords:
- Juglans mandshurica Maxim.; green peel; alkaloids; antioxidant; antibacterial
- 文献标志码:
- A
- 摘要:
- 以核桃楸外果皮为试材，采用超声辅助法及紫外可见分光光度法，研究了核桃楸外果皮总生物碱的最佳提取方法，并测定其抗氧化性和抑菌性，以期为核桃楸外果皮中生物碱的后续利用提供参考依据。结果表明：核桃楸外果皮生物碱最优提取条件为乙醇体积分数80%、料液比1∶10 g?mL-1、超声温度40 ℃、超声时间40 min。抗氧化试验结果表明，核桃楸外果皮的总生物碱提取物对羟基自由基、亚硝酸根离子、超氧阴离子均有清除作用，由线性回归结果得到3种由基的IC50值分别为0.030（?OH）、0.029（NO2-）、0.033 g?L-1（O?[TX-*4]2）。抑菌试验结果表明，核桃楸外果皮的总生物碱提取物对木材褐腐菌密粘褶菌和白腐菌彩绒革盖菌的生长均存在抑制作用。
- Abstract:
- Green peel of Juglans mandshurica was used as raw material，the optimal extraction method of the total alkaloid，and its antioxidant and antibacterial properties was studied by ultrasonic assisted and ultraviolet spectrophotometry in order to supply reference for using alkaloids of green peel of Juglans mandshurica.The results showed that the optimum extraction conditions generated by orthogonal tests were as follows,volume fraction of ethanol of 80%，solid-liquid ratio of 1∶10 g?mL-1，ultrasonic temperature of 40 ℃，and ultrasonic time of 40 minutes.In addition，the antioxidant tests showed that the total alkaloids of green peel of Juglans mandshurica had scavenging effect on hydroxyl radicals，nitrite ions and superoxide anion.The IC50 values calculated by linear-regression analysis were 0.030 g?L-1(?OH)，0.029 g?L-1(NO2-)，and 0.033 g?L-1(O?[TX-*4]2).The results of antibacterial tests showed that total alkaloids could control the growth of wood-rotting fungi of both Gloeophyllum trabeum and Coriolus versicolor.
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备注/Memo
第一作者简介：李玲（1992-），女，硕士研究生，研究方向为天然产物化学。E-mail：463037473@qq.com.责任作者：孙墨珑（1961-），女，博士，教授，研究方向为天然产物化学。E-mail：molongsun@126.com.基金项目：中央高校基本科研业务费专项资金资助项目（2572015BB18）。