ZHANG Zhilu,LIU Zhonghua,CHEN Minghui,et al.Physiology Response of Correa carmen Seedlings to Combined Elevated Temperature and Drought Stress[J].Northern Horticulture,2017,41(19):81-88.[doi:10.11937/bfyy.20170134]
高温干旱胁迫下考来木幼苗的生理响应
- Title:
- Physiology Response of Correa carmen Seedlings to Combined Elevated Temperature and Drought Stress
- Keywords:
- anti-oxidant enzyme activity; combined stress; Correa carmen; drought; elevated temperature; ROS metabolism
- 文献标志码:
- A
- 摘要:
- 以盆栽考来木幼苗为试材，采用人工气候室模拟高温环境的方法，研究了高温干旱复合胁迫对考来木保护性酶系统生理活性的影响，以期为考来木的栽培与推广、园林植物的抗逆育种等提供科学理论依据，为植物生理生态研究贡献微薄的力量。结果表明：干旱或高温单一胁迫下，考来木幼苗的H2O2、O?-2等活性氧代谢物含量及丙二醛（MDA）含量增加，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）等抗氧化性酶活性亦同步显著增高。在高温、干旱复合胁迫下，活性氧代谢物含量及抗氧化性酶活性均显著高于单一胁迫，SOD活性近乎叠加增长；较之单一胁迫，复合胁迫下MDA含量并无显著升高。随胁迫时间延长或胁迫程度增加，抗氧化性酶活性持续增强；当胁迫增加到一定程度或持续一定时间后，抗氧化酶活性显著下降。干旱胁迫对考来木的伤害大于高温胁迫。结果表明，考来木可通过大幅提高抗氧化性酶系统的活性来抵御高温干旱胁迫所产生的活性氧伤害，因而具有较强的耐高温干旱能力。但抗氧化酶活性的提高是有限的，高强度或长时间胁迫下活性氧大量积累，抗氧化性酶被破坏而活性下降，则作物受害。
- Abstract:
- Cultivated Correa carmen seedlings in pot as test material in phytotron to study the effects of combined stress of elevated temperature and drought on the physiological activities of the protective enzyme system of the seedlings,with a view to providing scientific theoretical basis for the cultivation and promotion of Correa carmen,and the anti-breeding of garden plants,a small contribution to the research of plant physiology and ecology.The results showed that,under the single stress of drought or high temperature,H2O2 content,O?-2 produce rate and MDA content of Correa carmen seedlings increased significantly,at the same time the activities of SOD,POD and CAT antioxidant enzyme also increased significantly.Under the composite stress of high temperature and drought,ROS content and antioxidant enzyme activities were significantly higher than that of single stress,SOD activity nearly superposition growth;compared with the single stress,MDA content had no significant change under complex stress.ROS content and antioxidant enzyme activities progressively increased along with the extension of stress time and the increase of the stress degree.Nevertheless,antioxidant enzyme activities decreased significantly when the stress increased to a certain degree or lasted a certain period.The hurt to Correa carmen of the stress of drought was significantly than elevated temperature.All above results showed that Correa carmen seedlings could significantly improve its activity of antioxidant enzyme system to defense ROS caused by stress of high temperature and drought,thus they had outstanding ability of resistance to high temperature and drought.But the degree increased of the antioxidant enzymes activity was limited,when the stress time extended or stress degree increased,reactive oxygen accumulated,the antioxidant enzyme system were destroyed and the activity decreased,so the plants hurt by ROS finally.
参考文献/References:
[1]LOW P S,MERIDA J R.The oxidative burst in plant defense:Function and signal transduction[J].Physiologia Plantarum,1996,96(3):533-542.
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备注/Memo
第一作者简介：张志录（1967-），男，博士，副教授，现主要从事园艺学及种群生态学等研究工作。E-mail：276996899@qq.com.