YI Shuangshuang,LU Shunjiao,YANG Guangsui,et al.Research Progress on Orchids Genetic Transformation Technology[J].Northern Horticulture,2016,40(20):187-194.[doi:10.11937/bfyy.201620049]
兰花遗传转化技术研究进展
- Title:
- Research Progress on Orchids Genetic Transformation Technology
- 文献标志码:
- A
- 摘要:
- 兰花是世界重要的盆栽观赏植物和切花花卉，利用转基因技术进行兰花基因工程育种和基因功能研究是近年来兰花育种的热点，而高效稳定的兰花遗传转化体系的建立是兰花基因工程育种的关键基础。现综述了近年来兰花遗传转化的研究进展，包括转化受体、转化方法、影响转化效率等相关因素，以期为兰花遗传转化技术的提高、转基因新品种的培育等研究提供参考。
- Abstract:
- Orchids is the very important potted ornamental plant as well as cut flower in the world，on which the engineering breeding and the gene function research by using transgenic technology is a hot spot of research in recent years.While，the establishment of stable and efficient genetic transformation system is the key foundation.So，in this study，we reviewed the research progress on orchids genetic transformation in recent years，including transformation acceptors，transformation methods，the influence factors of transformation efficiency，and so on.This will provide reference for the improvement of the genetic transformation technology and the genetically modified varieties breeding on orchids.
参考文献/References:
[1]ARDITTI J.Fundamentals of orchid biology[M].New York:Wiley,1992. [2]ALALM M K,RASHID M H,HOSSAIN M S,et al.In vitro seed propagation of Dendrobium (Dendrobium transparens) orchid as influenced by different media[J].Biotechnology,2002(1):111-115. [3]李杰.几种名贵洋兰转基因受体系统的建立及遗传转化[D].南京:南京林业大学,2005. [4]YU H,GOH C J.Differential gene expression during floral transition in an orchid hybrid Dendrobium Madame Thong-In[J].Plant Cell Reports，2000(19):926-931. [5]王关林,方宏筠.植物基因工程[M].2版.北京:科学出版社,2002:517-531. [6]YANG J,LEE H,SHIN D H,et al.Genetic transformation of Cymbidum orchid by particle bombardment[J].Plant Cell Reports,1999(18):978-984. [7]ISHII Y,TAKAMURA T,GOI M,et al.Callus induction and somatic embryogenesis of Phalaenopsis[J].Plant Cell Rep,1998(17):446-450. [8]CHEN J T,CHANG C,CHANG W C,et al.Direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey and subsequent plant regeneration[J].Plant Cell Rep,1999(19):143-149. [9]KUEHNLE A R,SUGII N.Transformation of Dendrobium orchid using particle bombardment of protocorms[J].Plant Cell Rep,1992(11):484-488. [10]MISHIBA K,CHIN D P,MII M.Agrobacterium-mediated transformation of Phalaenopsis by targeting protocorms at an early stage after germination[J].Plant Cell Rep,2005(24)：297-303. [11]TEE C S,MARZIAH M,TAN C S,et al.Evaluation of different promoters driving the GFP reporter gene and selected target tissues for particle bombardment of Dendrobium Sonia 17[J].Plant Cell Rep，2003(21):452-458. [12]LIAO L J,PAN I C,CHAN Y L，et al.Transgene silencing in Phalaenopsis expressing the coat protein of Cymbidium mosaic virus is a manifestation of RNA-mediated resistance[J].Mol Breed,2004(13):229-242. [13]CHAN Y L,LIN K H,SANJAYA-LIAO L J,et al.Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack[J].Transgenic Res,2005(14)：279-288. [14]张振华.石斛兰文心兰转CyMV-CP、ORSV-CP基因研究[D].海口:海南大学,2011. [15]UDDAIN J,ZAKARIA L,LYNN C B,et al.Preliminary assessment on Agrobacterium-mediated transformation of Dendrobium Broga Giant orchid′s PLBs[J].Emir J Food Agric,2015,27:669-677. [16]LIAU C H,LU J C,PRASAD V,et al.The sweet pepper ferredoxin-like protein (pflp) conferred resistance against soft rot disease in Oncidium orchid[J].Transgenic Res,2003a(12):329-336. [17]LI S H,KUOH C S,CHEN Y H,et al.Osmotic sucrose enhancement of single-cell embryogenesis and transformation efficiency in Oncidium[J].Plant Cell Tissue Organ Cult,2005,81:183-192. [18]BELARMINO M M,MII M.Agrobacterium mediated genetic transformation of a Phalaenopsis orchid[J].Plant Cell Reports,2000(19):435-442. [19]詹洲理,陈文辉,詹明才.蝴蝶兰基因转殖暂时性基因表现之最佳条件[J].中国园艺，2003，49(1)：33-44. [20]刘其府,董会,曾宋君,等.农杆菌子房注射法对金钗石斛的活体转化研究[J].华南农业大学学报,2013,34(3):378-382. [21]TEIXEIRA da SILVA J A,ACETO S,LIU W,et al.Genetic control of flower development,color and senescence of Dendrobium orchids[J].Sci Hortic，2014,175:74-86. [22]TEIXEIRA da SILVA J A,KERBAUY G B,ZENG S J,et al.In vitro flowering of orchids[J].Crit Rev Biotechnol,2014,34:56-76. [23]CHIA T F,CHAN Y S，CHUA N H.The firefly luciferase gene as a non-invasive reporter for Dendrobium transformation[J].Plant J,1994(6):441-446. [24]NAN G L,KUEHNLE A R.Factors affecting gene delivery by particle bombardment of Dendrobium orchids[J].In Vitro Cell Dev Biol-Plant,1995，31:131-136. [25]王微,杨晓伶,杨婧,等.根癌农杆菌介导大花蕙兰遗传转化的研究[J].西北植物学报,2011,31(1):27-32. [26]金苹.根癌农杆菌介导ACS反义基因转化文心兰的研究[D].福州:福建农林大学,2009. [27]YU Z,CHEN M,LIN N,et al.Recovery of transgenic orchid plants with hygromycin selection by particle bombardment to protocorms[J].Plant Cell Tiss Organ Cult，1999,58:87-92. [28]MEN S,MING X,LIU R,et al.Agrobacterium-mediated genetic transformation of a Dendrobium orchid[J].Plant Cell Tiss Organ Cult,2003,75:63-71. [29]KNAPP J E,KAUSCH A P,CHANDLEE J M.Transformation of three genera of orchid using the bar gene as a selectable marker[J].Plant Cell Reports,2000(19)：893-898. [30]YU H,YANG S H,GOH C J.Spatial and temporal expression of the orchid floral homeotic gene DOMADS1 is mediated by its upstream regulatory regions[J].Plant Mol Biol,2002,49:225-237. [31]HSIEH R M,CHEN W H,HSU H M,et al.Agrobacterium tumefaciens-mediated transformation of Phlaenopsis orchid[J].Report of Taiwan Sugar Research Institute,1997,155:41-54. [32]TEE C S,MAZIAH M.Optimization of biolistic bombardment for Dendrobium Sonia 17 calluses using GFP and GUS as the reporter system[J].Plant Cell Tiss Organ Cult,2005,80:77-89. [33]ATICHART P,BUNNAG S,THEERAKULPISUT P.Agrobacteriummediated transformation of Dendrobium secundum (Bl.) Lindl.with antisense ACC oxidase[J].Asian J Plant Sci,2007(6):1065-1071. [34]BUNNAG S,DUANGSEE K.Transformation of Dendrobium primulinum Lindl.with antisense ACC oxidase[J].Acta Hortic,2009,829:283-288. [35]TEE C S,MAZIAH M,TAN C S,et al.Selection of cotransformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein[J].Biol Plant,2011,55:572-576. [36]BUNNAG S,PILAHOME W.Agrobacterium-mediated transformation of Dendrobium chrysotoxum Lindl[J].Afr J Biotechnol,2012(11):2472-2476. [37]YOU S J,LIAU C H,HSIANG E H,et al.Sweet pepper ferredoxin-like protein (pflp) gene as a novel selection marker for orchid transformation[J].Planta，2003,217(1):60-65. [38]邵寒霜,李继红,王胜培.Lfy cDNA 高效单子叶植物表达载体的构建及转化兰花研究初报[J].热带作物学报,2000,21(3):58-62. [39]SUWANAKETCHANATIT C,CHAISUK P,PILUEK J,et al.Evaluation of constitutive promoters for gene expression in Dendrobium protocorms and flowers[J].Kasetsart J(Nat Sci),2006,40:934-943. [40]SUWANAKETCHANATIT C,PILUEK J,PEYACHOKNAGUL S，et al.High efficiency of stable genetic transformation in Dendrobium via microprojectile bombardment[J].Biol Plant,2007，51:720-727. [41]KOO J C.Isolation of an actin promoter for strong expression of transgenes in the orchid genus Dendrobium[J].Plant Biotechnol,2013，40:27-36. [42]HSIEH Y H,HUANG P L.Studies on genetic transformation of Phalaenopsis via pollen tube pathway[J].Journal of the Chinese Society for Horticultural Science,1995,41:309-324. [43]姚振.类胡萝卜素合成酶基因PSY、PDS、LycB、LycE对安祖花与蝴蝶兰的遗传转化[D].长春:吉林大学,2005. [44]满若君.蝴蝶兰、文心兰再生体系的建立及遗传转化体系的初步研究[D].南宁:广西大学,2007. [45]郝曜山,杜建中,孙毅.农杆菌介导转化蝴蝶兰遗传体系的研究[J].山西农业大学学报(自然科学版),2010(3):205-208. [46]王宇腾.蝴蝶兰ACC氧化酶和ACC合成酶基因融合反义表达载体的构建及遗传转化[D].郑州:河南农业大学,2011. [47]牛苏燕.蝴蝶兰ACC合成酶（ACS）反义基因植物表达载体的构建及遗传转化[D].郑州:河南农业大学,2012. [48]崔波,蒋素华,牛苏燕,等.农杆菌介导蝴蝶兰的遗传转化研究[J].河南农业大学学报,2012,46(6):642-645,650. [49]卢毕生.石斛兰高效再生体系的建立与抗冻蛋白（AFP）基因转化的研究[D].儋州:华南热带农业大学,2005. [50]聂小容.石斛兰花期和花色的转基因研究[D].成都:四川农业大学,2005. [51]杨雪飞.基因枪介导的LEA3基因对石斛的遗传转化研究[D].合肥:合肥工业大学,2007. [52]张妙彬,梁擎中,肖浩,等.农杆菌介导石斛兰遗传转化的研究[J].园艺学报,2008,35(4):565-570. [53]冯莹.石斛兰ACS反义基因的遗传转化及离体开花的研究[D].福州:福建农林大学,2008. [54]石彩娟.铁皮石斛NAC基因的遗传转化[D].成都:西南交通大学,2013. [55]张振华.石斛兰文心兰转CyMV-CP、ORSV-CP基因研究[D].海口:海南大学,2011. [56]LI S H,KUOH C S,CHEN Y H，et al.Osmotic sucrose enhancement of single-cell embryogenesis and transformation efficiency in Oncidium[J].Plant Cell Tissue Organ Cult,2005，81：183-192. [57]甘露.拟南芥CBF3基因转化文心兰的研究[D].海口:海南大学,2010. [58]GRIESBACH R J,HAMMOND J.Incorporation of GUS gene into orchids via embryo electrophoresis[J].Acta Hort，1993,336:165-169. [59]徐京.LjCYC1基因转化非洲紫罗兰及CFL基因转化仙客来、春兰×大花蕙兰杂种的研究[D].杭州:杭州师范大学,2011. [60]陈志俊,明小天,刘荣维,等.兰花圆球茎基因枪转化后的GUS基因表达[J].高技术通讯,2000(4):94-96. [61]STEINHART W L,WINKLER R J，BRIERLEY K L,et al.Expression of the β-glucuronidase reporter gene from a variety of promoter constructs in Cattleya protocorms and their protoplasts[J].Lindleyana,1997(12):172-179. [62]谢利,王芬,曾瑞珍,等.农杆菌介导的墨兰遗传转化[J].生物工程学报2015,31(4):542-551. [63]NAN G L,TANG C S,KUEHNLE A R,et al.Dendrobium orchids contain an inducer of Agrobacterium virulence genes[J].Physiological and Molecular Plant Pathogogy,1997，51:391-399. [64]YU H,YANG S H,GOH C J.Agrobacterium-mediated transformation of a Dendrobium orchid with the class 1 konx gene DOH1[J].Plant Cell Reports,2001,201:301-305. [65]LIAU C H,YOU S J,PRASAD V,et al.Agrobacterium tumefaciens-mediated transformation of an Oncidium orchid[J].Plant Cell Reports,2003b(21)：993-998. [66]柴明良,金斗焕.农杆菌介导的蝴蝶兰基因转化系统的建立[J].园艺学报,2004,31(4):537-539. [67]潘才博.蝴蝶兰ACS反义基因的遗传转化及ACS基因的克隆研究[D].福州:福建农林大学,2007. [68]NAN G L,KUEHNLE A R,KADO C I.Transgenic Dendrobium orchid through Agrobacterium-mediated transformation[J].Malay Orchid Rev,1998,32:93-96. [69]ANZAI H,ISHII Y,SHICHINOHE M,et al.Transformation of Phalaenopsis by particle bombardment[J].Plant Tiss Cult Lett，1996(13):265-271. [70]MEN S,MING X,WANG Y,et al.Genetic transformation of two species of orchid by biolistic bombardment[J].Plant Cell Reports,2003,21(6):592-598.
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备注/Memo
第一作者简介：易双双（1984-），女，硕士，研究实习员，现主要从事热带花卉遗传育种等研究工作。E-mail:yishuang8410@163.com.责任作者：尹俊梅（1968-），女，研究员，现主要从事热带花卉种质资源与遗传育种等研究工作。E-mail:yinjunmei2004@163.com.基金项目：中央级公益性科研院所基本科研业务费专项资助项目（1630032013004，1630032014014）。