CHEN Tianxiang,GU Xin,SUN Quan.Effect of Exogenous Phenolic Acids on Biomass Accumulation and Antioxidant System of Greenhouse Cucumber Seedlings[J].Northern Horticulture,2016,40(20):44-49.[doi:10.11937/bfyy.201620012]
外源酚酸对设施黄瓜幼苗生物量积累和抗氧化系统的影响
- Title:
- Effect of Exogenous Phenolic Acids on Biomass Accumulation and Antioxidant System of Greenhouse Cucumber Seedlings
- Keywords:
- autotoxicity; cinnamic acid; p-hydroxybenzoic acid; antioxidant system; growth of seedling
- 文献标志码:
- A
- 摘要:
- 针对设施黄瓜连作障碍中的自毒作用，以“津春5号”黄瓜幼苗为试材，以灭菌石英砂为基质，施加不同浓度的酚酸进行砂培试验，探讨酚酸类物质对黄瓜幼苗生物量积累、根系生长发育及叶片抗氧化系统的影响。结果表明：50 mg?kg-1苯丙烯酸对叶绿素含量和茎粗具有显著促进作用，100~150 mg?kg-1对羟基苯甲酸有利于叶绿素含量增加，浓度增高抑制作用增强。2种酚酸浓度为30~50 mg?kg-1时，促进地上部鲜质量增加，但差异不显著。酚酸浓度升高对地上部鲜质量抑制作用逐渐增强。50 mg?kg-1苯丙烯酸显著促进根冠比增加且有利于根系生长。50 mg?kg-1对羟基苯甲酸对幼苗地下部鲜质量、主根长和根体积抑制作用显著。50~150 mg?kg-1酚酸处理显著促进超氧化物歧化酶（SOD）活性的增加。2种酚酸浓度为30~150 mg?kg-1对过氧化物酶（POD）活性有相似效应，大于200 mg?kg-1对POD活性具有显著促进作用，质膜透性和丙二醛（MDA）含量也随之升高。由此可见，砂培条件下，低浓度（30~50 mg?kg-1）酚酸对黄瓜幼苗生长发育具有促进作用，高浓度（≥200 mg?kg-1）酚酸具有抑制作用，且不同酚酸对黄瓜幼苗的生理效应浓度不同。
- Abstract:
- For the autotoxicity of greenhouse cucumber under continuous cropping，taking ‘Jinchun 5’ cucumber seedlings as test materials to do experiment of sand culture with exogenous phenolic acids of different concentration，the impact on biomass accumulation of cucumber seedlings，growth of root and antioxidant system of leaf were discussed.The results showed that chlorophyll content and stem diameter were promoted significantly by cinnamic acid of 50 mg?kg-1,chlorophyll content was promoted by the p-hydroxybenzoic acid of 100-150 mg?kg-1 significantly，this function was suppressed with high concentration.The aboveground fresh weight was enhanced by phenolic acids of 30-50 mg?kg-1 obviously，but no significant diversity.The concentration of 50 mg?kg-1 cinnamic acid had a promoting significant role for the root/shoot ratio and was favorable to growth of root，this function was suppressed with high concentration.However，p-hydroxybenzoic acid of 50 mg?kg-1 had significant inhibition on root fresh weight，root length and root volume.The concentration of 50-150 mg?kg-1 had no obvious stimulation for the superoxide dismutase (SOD).Two kinds of phenolic acids 30-150 mg?kg-1 had similar effect on the peroxidase (POD) activity.When the concentration of phenolic acids was 200 mg?kg-1 and more，enhanced POD activity significantly，membrane permeability and malondialdehyde (MDA) content also increased significantly under the high concentration.Therefore，cucumber seedlings had a certain tolerance to low concentration (30-50 mg?kg-1) phenolic acids，and high concentration (200 mg?kg-1) inhibit the growth and development of cucumber seedlings，the adaptability concentration ranges of physiological effects was various.
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备注/Memo
第一作者简介：陈天祥（1991-），男，硕士研究生，研究方向为干旱区土壤资源管理。E-mail：1282896413@qq.com.责任作者：顾欣（1973-），女，硕士，副教授，现主要从事农业微生物资源与利用等研究工作。E-mail：sqnxu@sina.com.基金项目：2015年宁夏农业科技支撑资助项目（nxnz-08）；2016年宁夏大学研究生创新资助项目（GIP201649）。