ZHAO Jie,ZHI Yuee,ZHAO Baoming,et al.Biological Characteristics of Colletotrichum gloeosporioides (Penz.) Sacc.and Toxicities of Fungicides[J].Northern Horticulture,2016,40(14):126-129.[doi:10.11937/bfyy.201614031]
无花果炭疽病菌生物学特性及药剂的毒力测定
- Title:
- Biological Characteristics of Colletotrichum gloeosporioides (Penz.) Sacc.and Toxicities of Fungicides
- Keywords:
- Colletotrichum gloeosporioides (Penz.) Sacc.; biological characteristics; toxicities of fungicides
- 文献标志码:
- A
- 摘要:
- 以无花果炭疽病菌（Colletotrichum gloeosporioides (Penz.) Sacc.）为供试菌，采用菌落生长速率法和测定产孢量的方法，研究了无花果炭疽病菌的生物学特性，并测定了8种杀菌剂的毒力。结果表明：PSA培养基最适宜菌落生长，葡萄糖蛋白胨琼脂培养基产孢量最多。菌丝生长的适宜温度范围为5~35 ℃，最适温度25 ℃；菌落产孢能适应较广的pH，以pH 3产孢量最多；菌丝的致死温度为56 ℃；菌落生长和产孢对光照的要求不严格。菌丝生长最适宜的碳、氮源分别为麦芽糖和蛋白胨；最适宜产孢的碳、氮源分别为果糖和牛肉膏。8种杀菌剂的毒力测定结果表明，咪鲜胺锰盐、多菌灵、氟啶胺和吡唑醚菌酯的毒力较高。
- Abstract:
- Taking Colletotrichum gloeosporioides (Penz.) Sacc.as test fungi，using the mycelium growth rate method and the determination of spore production，the biological characteristics of Colletotrichum gloeosporioides (Penz.) Sacc.were studied and the toxicity of 8 fungicides were tested.The results showed that the suitable media for mycelial growth and spore production were PSA and dextrose peptone agar medium，respectively.The suitable temperature for the mycelial growth was 5-35 ℃ and the best was 25 ℃.The optimum pH was 3 for the spore production.The thermal death temperature of the mycelia was 56 ℃.Colony growth and spore production could adapt to the different illumination conditions.The most favorable carbon source and nitrogen source for mycelia growth were maltose and peptone，respectively.The most favorable carbon source and nitrogen source for spore production were fructose and beef extract，respectively.The toxicity tests showed that prochloraz-manganese chloride complex，carbendazim，fluazinam and pyraclostrobin had higher toxicity on C.gloeosporioides.
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备注/Memo
第一作者简介：赵杰（1979-），男，硕士，高级农艺师，研究方向为园艺作物栽培与植保。E-mail：zhaocaoyou@163.com.基金项目：上海市科技兴农推广资助项目（沪农推字（2015）第1-2号)。