(Institute of Biology,Hebei Academy of Sciences,Shijiazhuang,et al.Induced Resistance of Cucumber Against Botrytis cinerea by Bacillus subtilis BSD-2 CHEN Yanguang,YIN Shuli,LIU Hongwei,ZHANG Genwei,CHENG Huicai,ZHANG Liping[J].Northern Horticulture,2016,40(12):119-123.[doi:10.11937/bfyy.201612029]
枯草芽孢杆菌BSD-2诱导黄瓜抗灰霉病的作用研究
- Title:
- Induced Resistance of Cucumber Against Botrytis cinerea by Bacillus subtilis BSD-2 CHEN Yanguang,YIN Shuli,LIU Hongwei,ZHANG Genwei,CHENG Huicai,ZHANG Liping
- 关键词:
- 枯草芽孢杆菌BSD-2; 灰霉病; 防御酶
- Keywords:
- Bacillus subtilis BSD-2; grey mould; defense enzyme
- 文献标志码:
- A
- 摘要:
- 以枯草芽孢杆菌BSD-2为供试菌，黄瓜品种“津春4号”为试材，采用生理生化方法，研究了BSD-2发酵液、抗菌肽及芽孢稀释溶液对叶片过氧化物酶（POD)、多酚氧化酶（PPO）、苯丙氨酸解氨酶（PAL）、超氧化物歧化酶（SOD）活性的影响；采用电泳法测定POD同工酶和叶片胞间隙蛋白质的变化。结果表明：处理后的酶活均在第5天达到高峰，发酵液处理对酶活性的提高作用明显。POD同工酶的变化不仅表现在同种同工酶表达量的改变，同工酶种类也相应地增加，叶片胞间隙蛋白电泳中出现大小约为27 kDa的新增蛋白条带，并且发酵液处理第11天时新增蛋白出现表达量的积累。表明枯草芽孢杆菌BSD-2及其抗菌物质可以通过提高防御酶活性，促进POD同工酶和胞间隙相关蛋白质的表达，从而诱导黄瓜产生对灰霉病的抗性作用。
- Abstract:
- Taking Bacillus subtilis BSD-2 as test bacteria,the cucumber variety ‘ Jinchun No.4’ as test material,the effects of fermented liquid,antibacterial peptides,spore dilute solution of BSD-2 on the activities of POD,PPO,PAL,SOD in cucumber leaves were determinated by using physiological and biochemical methods.The changes of POD isozyme and leaf intercellular space proteins were also analyzed by SDS-PAGE.The result showed that the activities of POD,PPO,PAL and SOD in cucumber leaves increased significantly after the treatments of fermented liquid,antibacterial peptide and spore dilute solution,then reached the peak on the fifth day.POD isozyme expression increased both in amount and kinds.SDS-PAGE analysis suggested that a new 27 kDa protein was expressed.All aboves showed that B.subtilis BSD-2 and its antibacterial substance could improve the defense enzymes activities,and promote the expression of the POD isozyme and intercellular space related proteins,and induce resistance of cucumber to grey mould.
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备注/Memo
第一作者简介：陈艳光(1989-)，女，硕士研究生，研究方向为应用微生物。E-mail:lhwei1987@126.com.责任作者：张丽萍(1969-)，女，硕士，研究员，研究方向为农业微生物。E-mail:lizzle-69@163.com.基金项目：河北省重点基础研究资助项目（13966503D）；河北省科学院重点资助项目（15302）；河北省科学院高层次人才资助项目（20150503LR62-11）。