ZHANG Xiaomei,ZHU Weihong,MIAO Xiaoyan,et al.Grinding Enzymolysis Purification and Microscopic Observation of Germination Characteristics on Chlamydospore of Agrocybe chaxingu[J].Northern Horticulture,2016,40(07):130-133.[doi:10.11937/bfyy.201607032]
茶薪菇厚垣孢子研磨酶解纯化与显微萌发特征观察
- Title:
- Grinding Enzymolysis Purification and Microscopic Observation of Germination Characteristics on Chlamydospore of Agrocybe chaxingu
- 文献标志码:
- A
- 摘要:
- 以茶薪菇SM菌株为试材，研究了4种分离纯化方法对厚垣孢子分离纯化的影响，并观察其显微萌发特征。结果表明：研磨酶解方法较好，在1.0%蜗牛酶和1.0%纤维素酶，30℃、4 h条件下，菌丝碎片酶解消失，孢子饱满，数量达1.9×105个/mL；显微镜检发现多数厚垣孢子萌发点位于孢子侧面，萌发菌丝具锁状联合结构，萌发后原孢子的液泡体积增大，胞内充满大液泡。
- Abstract:
- With SM strain of Agrocybe chaxingu as the experimental material，by comparative studies of four separation methods and purification of Agrocybe chaxingu chlamydospore were conducted and its germination characteristics were observed.The results showed that the grinding enzymolysis method yielded better results under the condition of 1.0% snailase，1.0% cellulase，30℃ and 4 hours，the hyphal fragments enzymatically decomposed and disappeared，and the spores appeared plumpy with concentration up to 1.9×105/mL.Microscopic observations results found that the most chlamydospore germination happened on the flanks of the spore，and mycelium germinated with joint structures in lock shapes.The volumes of the spore vacuoles increased since germination while the spores were filled with large vacuoles.
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备注/Memo
第一作者简介：张筱梅(1957-),女,本科，教授,研究方向为食药用真菌应用。E-mail:zhxm06@163.com. 基金项目：河北省自然科学基金资助项目(C2013104055)。