LU Yanxin,WANG Xue,QU Baihong.Clone and Sequence Analysis of PyMYB114 Gene cDNA in Pyrus bretschneideri Rehd. ‘Pingguoli’[J].Northern Horticulture,2016,40(06):89-91.[doi:10.11937/bfyy.201606022]
“苹果梨”PyMYB114基因cDNA全长的克隆与序列分析
- Title:
- Clone and Sequence Analysis of PyMYB114 Gene cDNA in Pyrus bretschneideri Rehd. ‘Pingguoli’
- 文献标志码:
- A
- 摘要:
- 以延边“苹果梨”果实为试材，采用同源克隆的试验方法，研究了果实着色过程的转录基因PyMYB114，并对其进行生物信息学分析。结果表明：PyMYB114的cDNA全长为744 bp，其包含1个完整的编码201〖KG-*4〗个氨基酸的ORF，其编码蛋白的分子量约为23.48 kDa，理论等电点(pI)为9.57。同源性分析表明，PyMYB114〖KG-*4〗基因与白梨的MYB114基因核酸序列的相似性高达99%。系统进化树表明，PyMYB114编码的氨基酸和白梨的MYB114编码的氨基酸在进化上亲缘关系最近。
- Abstract:
- Taking the Pyrus bretschneideri Rehd. ‘Pingguoli’ in Yanbian as test material，transcription gene PyMYB114 during the pigmentation of ‘Pingguoli’ were researched by using homologous cloning and bioinformatics technology.The results showed that the cDNA of PyMYB114 was 744 bp in length,encoded 201 amino acids with a calculated molecular weight(MV) of 23.48 kDa and isoelectric point(pI) of 9.57.Homology analysis revealed that the similarity between these cDNA fragments and the genes of other rosaceous plants was up to 99%.Phylogenetic tree indicated that amino acids encoded by PyMYB114 and Pyrus bretschneideri were the most closed on the evolution.
参考文献/References:
[1]李秀菊,林涛,刘用生等.红色苹果色素形成生理研究[J].园艺学报,1997,32(3):2-6.〖LL〗 [2]RAE R N,LEE S K.Influence of chlorophyll,internal ethyleteand PAL on athocyanin synthesis in Fuji apple[J].Korean Society Hort Sci,1995,36(3):361-370. [3]LANCASTER J E,GRANT J E,GAROLGN E L.Skin color,in apple-influence of copigmetation and plastid pigments on shade and darkness of red color in five genotypes[J].Amer Soc Hort Sci,1994,119:63-69. [4]孙百灵,曲柏宏,李伟,等.‘苹果梨’果实着色相关酶基因片段克隆及表达分析[J].吉林农业大学学报,2012,34(4):423-427,442. [5]杨宏霞,刘冰雁,刘振坤,等.‘苹果梨’果实着色过程中PyCHI和PyF3H的克隆与表达分析[J].果树学报,2015(3):359-365,521. [6]杨宏霞,曲柏宏,刘振坤,等.延边苹果梨PyDFR基因的克隆及表达分析[J].北方园艺,2015(4):99-103. [7]崔道雷.云南红梨果皮转录因子MYB、bHLH和WD40互作及调控果皮着色机理研究[D].昆明:昆明理工大学,2013. [8]陈清,汤浩茹,董晓莉,等.植物Myb转录因子的研究进展[J].基因组学与应用生物学,2009(2):365-372. [9]DELUC L,BARRIEU F,MARCHIVE C,et al.Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway[J].Plant Physiol,2006,140:499-511. [10]XIE D Y,SHASHI S B,WRIGHT E,et al.Metabolic engineering of proanthocyanidins through co-expression of anthocy-anidin reductase and the PAP1 MYB transcription factor[J].Plant J,2006,45:895-907.
备注/Memo
第一作者简介：鹿艳新（1988-），女，硕士研究生，研究方向为果树生理学。E-mail：395297341@qq.com.责任作者：曲柏宏（1963-），男，博士，教授，博士生导师，现主要从事园艺植物栽培与生理等研究工作。E-mail：bhqu211@ybu.edu.cn. 基金项目：国家自然科学基金资助项目（31160389）。