Louisiana iris,Pb stress,accumulation,et al.Effect of Pb stress on Pb Accumulation and Non-protein Thiol Content in Louisiana Iris[J].Northern Horticulture,2015,39(21):77-82.[doi:10.11937/bfyy.201521022]
铅胁迫下路易斯安那鸢尾铅吸收量和非蛋白巯基的变化
- Title:
- Effect of Pb stress on Pb Accumulation and Non-protein Thiol Content in Louisiana Iris
- Keywords:
- Louisiana iris; Pb stress; accumulation; non-protein thiol; GSH; Cys
- 文献标志码:
- A
- 摘要:
- 以路易斯安那鸢尾为试材，采用营养液培养方法，对铅（Pb）胁迫下路易斯安那鸢尾品种铅吸收量和非蛋白巯基（NPT）的变化等进行了研究。结果表明：路易斯安那鸢尾品种‘Professor Neil’对铅吸收和富集量相对较大且耐性最强。随着Pb浓度的升高，叶片、根状茎和根的Pb含量增加，增加量依次为根>根状茎>叶片。根在高浓度1 200 mg/L Pb时达到最大，其含量为108 366 mg/kg DW；根状茎在800 mg/L Pb时达到最大，其含量为7 966 mg/kg DW，呈先缓慢上升再下降的趋势；叶片在高浓度800 mg/L Pb时达到最大，其含量为4 011 mg/kg DW。随着Pb浓度的升高，根系耐受指数（IT）逐渐下降，在Pb含量大于600 mg/L后存在显著性差异。根至茎转移率（R/R）呈先上升再下降的趋势，在800 mg/L Pb 处理时达到最大值10.02%。茎至叶转移率（R/L）呈先上升再下降的趋势，在600 mg/L Pb 处理时达到最大值57.78%。Pb胁迫下随着0~600 mg/L浓度的升高和1~7 d的时间推移，GSH和Cys含量呈增加的趋势并产生显著差异，尤其GSH起着重要的Pb解毒作用。
- Abstract:
- Taking Louisiana iris as material，the effects of lead (Pb) stress on Pb content and the contents of non-protein thiols (NPT) in roots，rhizomes and leaves of Louisiana iris were studied by hydroponic culture.The results showed that Louisiana iris variety ‘Professor Neil’ accumulated and enriched relatively large amount of Pb and was the highest Pb tolerant variety.Pb contents in leaves，rhizomes and roots increased with the Pb level enhanced in the liquid and the content order from highest to the least were root>rhizome>leaf.Pb content in the leaves of ‘Professor Neil’ under 800 mg/L Pb treatment were the maximum and reached to 4 011 mg/kg DW.Pb transfer rate (R/R) from root to rhizome showed a trend of rise first and then dropped and in 800 mg/L Pb treatment maximum and reached 10.02%，and Pb transfer rate (R/L) from rhizome to leaf showed similar trend with R/R，in 600 mg/L Pb reached the maximum and was 57.78%.As the Pb concentrations (0-600 mg/L) increased and the stress time prolonged，the contents of GSH and Cys were significantly increased，showing these biochemical compounds might play an important role in Pb detoxification.
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备注/Memo
第一作者简介：田松青（1974-），女，硕士，副教授，高级工程师，现主要从事观赏植物资源与环境修复等研究工作。E-mail：tiansongqing@126.com.责任作者：朱旭东（1972-），男，博士，教授，现主要从事花卉育种和产业化等研究工作。E-mail：13862130525@163.com.基金项目：江苏省教育厅‘青蓝工程’科技创新团队资助项目（2014-24）；江苏省教育厅‘青蓝工程’中青年学术带头人资助项目（2012-246）；苏州市科技支撑计划资助项目（SNG201209）。