JIA Qian,GU Peiwen,QI Hexing,et al.Identification and Bio-control Technology Research to Putrefying Fungi of Grape in Eastern Helan Mountain of Ningxia[J].Northern Horticulture,2015,39(20):108-113.[doi:10.11937/bfyy.201520027]
宁夏贺兰山东麓酿酒葡萄果实致腐病菌鉴定及生物防治技术研究
- Title:
- Identification and Bio-control Technology Research to Putrefying Fungi of Grape in Eastern Helan Mountain of Ningxia
- Keywords:
- grape; putrefying fungi; biocontrol strain; control effect
- 文献标志码:
- A
- 摘要:
- 以葡萄病果为试材，采用组织分离、致病性检测等方法获得了4株引起致腐性病害的病原菌，分别为Rhizopus stolonifer、Penicillium expansum、Botrygis cinerea Pers和Colletotnchum gloeosprioides；并对其室内抑菌高活性菌株进行了筛选。结果表明：BM-木霉对4株致腐菌株均有较好的抑菌活性，最高抑菌率为79.78%，EC50值为1.38，YWZKDS4对4株致腐菌株的抑菌宽度均≥9.86 mm，BM-木霉和YWZKDS4均具有广谱抗菌活性。田间药效试验表明，BM-木霉、YWZKDS4和阳性对照药剂戊唑醇的防效分别为55.80%、61.27%和68.76%，并与其它药剂之间有显著性差异。
- Abstract:
- Taking diseased grape fruits as test materials，pathogenic bacteria were isolated and identified，the activity strains，which could highly inhibit pathogenic bacteria were also screened by using laboratory experiments.The results showed that pathogenic bacteria which caused rot disease of grape were Rhizopus stolonifera，Penicillium expansum，Botrygis cinerea Pers and Colletotnchum gloeosprioides；BM-trichoderma had high antibacterial activity on four putrefying fungi strains，moreover，the highest antibacterial rate was 79.78% and EC50 value was 1.38.BM-trichoderma and YWZKDS4 had broad-spectrum antibacterial activity，and the inhibition zone of YWZKDS4 against four putrefying fungi strains exceed to 9.86 mm.Field experiments showed that the control effects of BM-trichoderma,YWZKDS4 and positive control drug were 55.80%,61.27% and 68.76%，and there were significant differences with other agents.
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备注/Memo
第一作者简介：贾倩（1989-）,女,硕士研究生,研究方向为葡萄与葡萄酒。E-mail：1270337389@qq.com.责任作者：张军翔(1971-)，男，博士，教授，研究方向为葡萄与葡萄酒。E-mail：zhangjunxiang@126.com.基金项目：国家科技支撑计划资助项目（2013BAD09B02）；宁夏回族自治区科技支撑计划资助项目（NG2013）。