MENG Fu-xuan,ZHOU Jun,XIN Pei-rao,et al.The Research Status of Anthocyanin Biosynthesis and Regulation in Apple[J].Northern Horticulture,2015,39(07):180-187.[doi:10.11937/bfyy.201507050]
苹果花色素苷生物合成及调控的研究现状
- Title:
- The Research Status of Anthocyanin Biosynthesis and Regulation in Apple
- Keywords:
- apple; anthocyanin; biosynthesis; gene regulation; environmental factors
- 文献标志码:
- A
- 摘要:
- 苹果果实颜色是影响消费者需求重要的品质性状，苹果的红色是由花色素苷积累水平决定的，花色素苷是次生代谢产物黄酮类化合物中的一类。文章综述了花色苷合成过程中结构基因、调控基因、环境因素、管理措施和组织机构对花色苷积累的影响，目的是为实现优质果实品质提供新的方法，包括视觉上的吸引力和营养价值的提升。
- Abstract:
- The color of apple is an important quality characters could affect consumer demand.The red of apple peel is determined by the anthocyanin accumulation level,and the anthocyanin belong to flavonoids is a kind of metabolites. This article summraized the factors in anthocyanin synthesis accumulated which was included structural genes, regulatory genes, environmental factor, management and organization measure,and the purpose was to increase the visual appeal of apple, and provide a new method for improving the nutritional value.
参考文献/References:
[1]Adriana T，Bradeen J M，Luby J J,et al.Regulation of anthocyanin accumulation in apple peel[J].Horticultural Reviews,2011，38:357-391. [2]Korban S S，Wannarat W，Rayburn C M，et al.Genome size and nucleotypic variation in Malus germplasm[J].Genome,2009，52：148-155. [3]Chinnici F，Bendini A，Gaiani A，et al.Radical scavenging activities of peels and pulps from cv.Golden Delicious apples as related to their phenolic composition[J].J Agric Food Chem，2004，52：4684-4689. [4]Saure M C.External control of anthocyanin formation in apple：A review[J].Sci Hort,1990，42:181-218. [5]King M C，Cliff M A.Development of a model for prediction of consumer liking from visual attributes of new and established apple cultivars[J].J Am Pom Soc,2002，56：223-229. [6]Chagne D，Carlile C M，Blond C，et al.Mapping a candidate gene (MdMYB10) for red flesh and foliage colour in apple[J].BMC Genomics，2007，8:212-222. [7]Geralding Wamer.New apple varieties developed by Swiss breeder Markus Kobelt are red from the skin to the core[EB/OL].http://www.goodfruit.com/Good-Fruit-Grower/Oct-ober-2010/To-tally-RED/，Good Fruit Growe，2010. [8]Iris S，Henryk F，Li H H，et al.Shift in polyphenol prowler and sublethal phenotype caused by silencing of anthocyanidin synthase in apple[J].Planta，2009，229(3)：681-692. [9]Grot E E.The genetics and biochemistry of floral pigments[J].Annu Rev Plant Biol,2006，57:761-780. [10]Martens S，Knott J，Seitz C A，et al.Impact of biochemical prestudies on specific metabo-lic engineering strategies of flavonoid biosynthesis in plant tissues[J].Biochemical Engineering J，2003，14：227-235. [11]Gould K S.Natures swissarmy knife：The diverse protective roles of anthocyanins in leaves[J].J Biomed Biotechnol,2004，314-320. [12]高龙燕,罗轩,范飞,等.不同芒果品种嫩叶叶色的聚类分析[J].中国南方果树,2012,41(5):57-59. [13]Tsao R，Yang R，Young J C，et al.Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC)[J].J Agr Food Chem,2003，51:6347-6353. [14]Tsao R，Yang R，Xie S，et al.Which polyphenolic compounds contribute to the total antioxidant activities of apple?[J].J Agr Food Chem,2005，53:4989-4995. [15]Boyer J，Liu R H.Apple phytochemicals and their health benefits[J].Nutr J,2004，3:5. [16]Lata B.Relationship between apple peel and the whole fruit antioxidant content：Year and cultivar variation[J].J Agr Food Chem,2007，55:663-671. [17]Cabe P R，Baumgarten A，Onan K，et al.Using microsatellite analysis to verify breeding records：a study of ‘Honeycrisp’ and other cold-hardy apple cultivars[J].Hort Science，2005，40:15-17. [18]Telias A.Studies on apple peel color regulation[D].University of Minnesota，2009:55. [19]Bae R，Kim K.Anatomical observations of anthocyanin rich cells in apple skins[J].Hort Science，2006，41:733-736. [20]Rebecca A，Henry-Kirk，Tony K，et al.Transcriptional analysis of apple fruit proanthocyanidin biosynthesis[J].Journal of Experimental Botany,2012，63(15)：5437-5450. [21]Takos A M，Robinson S P，Walker A R.Transcriptional regulation of the flavonoid pathway in the skin of dark grown ‘Cripps’ Red’ apples in response to sunlight[J].J Hort Sci Biotechnol，2006b，81:735-744. [22]Allan A C，Hellens R P，Laing W A.MYB transcription factors that colour our fruit[J].Trends Plant Sci,2008，13：99-102. [23]Espley R V，Hellens R P，Putterill J，et al.Red colouration in apple fruit is due to the activity of the MYB transcription factor，MdMYB10[J].Plant J，2007，49:414-427. [24]Honda C，Kotoda N，Wada M，et al.Anthocyanin biosynthetic genes are coordinately expressed during red coloration in apple skin[J].Plant Physiol Biochem,2002，40:955-962. [25]Ju Z，Yuan Y，Liou C，et al.Relationships among phenylalanine ammonialyase activity，simple phenol concentrations and anthocyanin accumulation in apple[J].Sci Hort，1995b，61:215-226. [26]Takos M，Jaffe F W，Jacob S R，et al.Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples[J].Plant Physiology,2006，142:1216-1232. [27]Kondo S，Hiraoka K，Kobayashi S，et al.Changes in the expression of anthocyanin biosynthetic genes during apple development[J].J Am Soc Hort Sci，2002，127:971-976. [28]Ban,Y，Honda C，Bessho H，et al.Suppression subtractive hybridization identifies genes induce in response to UV-B irradiation in apple skin：Isolation of a putative UDP-glucose 4-epimerase[J].J Expt Bot，2007a，58:1825-1834. [29]Han Y P，Vimolmangkangs,Soria-Guerra R E，et al.Ectopic expression of apple F3’H genes contributes to anthocyanin accumulation in the arabidopsis tt7 mutant grown under nitrogen stress[J].Plant Physiology，2010，153：806-820. [30]Han Y P，Vimolmangkangs,Soria-Guerra R E，et al.Introduction of apple ANR genes into tobacco inhibits expression of both CHI and DFR genes in flowers，leading to loss of anthocyanin[J].Journal of Experimental Botany，2012，63(7)：2437-2447. [31]An X，Tian Y，Chen K，et al.The apple WD40 protein MdTTG1 interacts with bHLH but not MYB proteins to regulate anthocyanin accumulation[J].Journal of Plant Physiology，2012，169(7)：710-718. [32]Gonzalez A，Zhao M，Leavitt J，et al.Regulation of the anthocyanin biosynthetic pathway by the ttg1/bHLH/Myb transcriptional complex in Arabidopsis seedlings[J].Plant J,2008，53:814-827. [33]Takos A M，Jaffe F W，Jacob S R，et al.Lightinduced expression of a MYB gene regulates anthocyanin biosynthesis in red apples[J].Plant Physiol,2006a，142:1216-1232. [34]Ban Y，Honda C，Hatsuyama Y，et al.Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin[J].Plant Cell Physiol，2007，48：958-970. [35]Brueggemann J，Weisshaar B，Sagasser M.A WD40-repeat Gene from Malus×Domestica is a functional homologue of Arabidopsis thaliana transparent testa glabral[J].Plant Cell Rep，2010，29:285-294. [36]Espley R，Brendolise C，Chagne D，et al.Multiple repeats of a promoter segment causes transcription factor autoregulation in red apples[J].Plant Cell,2009,10:1105. [37]Feng X M，Zhao Q，Zhao L L，et al.The cold-induced basic helix-loop-helix transcription factor gene MdCIbHLH1 encodes an ICE-like protein in apple[J].BMC Plant Biology,2012，12(22)：1-14. [38]Matsui K，Umemura Y，Ohme-Takagi M.AtMYBL2，a protein with a single MYB domain，acts as a negative regulator of anthocyanin biosynthesis in Arabidopsis[J].Plant J,2008，55:954-967. [39]Jin H E，Cominelli P，Bailey A，et al.Transcriptional repression by AtMYB4 controls production of UVprotecting sunscreens in Arabidopsis[J].Embo J,2000，19:6150-6161. [40]Marais E，Jacobs G，Holcroft D M.Colour response of ‘Cripps’ Pink’ apples to postharvest irradiation is influenced by maturity and temperature[J].Sci Hort,2001，90:31-41. [41]Reay P F，Lancaster J E.Accumulation of anthocyanins and quercetin glycosides in ‘Gala’ and ‘Royal Gala’ apple fruit skin with UV-B-Visible irradiation：Modifying effects of fruit maturity，fruit side，and temperature[J].Sci Hort,2001，90:57-68. [42]Bakhshi D，Arakawa O.Induction of phenolic compounds biosynthesis with light irradiation in the flesh of red and yellow apples[J].J Appl Hort,2007，9:101-104. [43]Telias A，Rother D，Hoover E.Plant and environmental factors influencing the pattern of pigment accumulation in ‘Honeycrisp’ apple peels，using a novel color analyzer software tool[J].Hort Science，2008，43:1321-1627. [44]Ubi B E，Honda C，Bessho H，et al.Expression analysis of anthocyanin biosynthetic genes in apple skin：Effect of UV-B and temperature[J].Plant Sci,2006，170:571-578. [45]Xie X B，Li S，Zhang R F.The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples[J].Plant，Cell and Environment，2012，35：1884-1897. [46]Awad M A，De Jager A.Relationships between fruit nutrients and concentrations of flavonoids and chlorogenic acid in ‘Elstar’ apple skin[J].Sci Hort，2002b，92:265-276. [47]Strissel T，Halbwirth H，Hoyer U，et al.Growthpromoting nitrogen nutrition affects flavonoid biosynthesis in young apple (Malus domestica Borkh.) leaves[J].Plant Biol,2005，7:677-685. [48]Peng M，Hudson D，Schofield A，et al.Adaptation of Arabidopsis to nitrogen limitation involves induction of anthocyanin synthesis which is controlled by the NLA gene[J].J ExpT Bot,2008，59:2933-2944. [49]Funke K，Blanke M.Monophosphate and extenday improved apple colouration and firmness[J].Erwerbsobstbau，2006，48:121-129. [50]Teng S，Keurentjes J，Bentsink L，et al.Sucrose-specific induction of anthocyanin biosynthesis in Arabidopsis requires the MYB75/PAP1 gene[J].Plant Physiol,2005，139:1840-1852. [51]Rowan D D，Cao M，Linwang K，et al.Environmental regulation of leaf colour in red 35S:PAP1 Arabidopsis thaliana[J].New Phytologist，2009，182:102-115. [52]Whale S K，Singh Z.Endogenous ethylene and color develpment in the skin of ‘Pink Lady’ apple[J].J Am Soc Hort Sci,2007，132:20-28. [53]Awad M A，Anton de J.Formation of flavonoids，especially anthocyanin and chlorogenic acid in ‘Jonagold’ apple skin：Influences of growth regulators and fruit maturity[J].Sci Hort,2002a，93:257-266. [54]Whale S K，Singh Z，Behboudian M H，et al.Fruit quality in ‘Cripp’s Pink’ apple，especially colour，as affected by preharvest sprays of aminoethoxyvinylglycine and ethephon[J].Sci Hort,2008，115:42-351. [55]Li Z，Gemma H，Iwahori S.Stimulation of ‘Fuji’ apple skin color by ethephon and phosphorus-calcium mixed compounds in relation to flavonoid synthesis[J].Sci Hort,2002，94:193-199. [56]Mata A P，Val J，Blanco A.Differential effects of prohexadione-calcium on red colour development in ‘Royal Gala’ and ‘Fuji’ apples[J].J Hort Sci Biotechnol，2006，81:84-88. [57]Kondo S.The roles of jasmonates in fruit color development and chilling injury[J].Acta Hort,2006，727:45-53. [58]Rudell D R，Mattheis J P.Synergism exists between ethylene and metyl jasmonate in artificial light-induced pigment enhancement of ‘Fuji’ apple[J].Postharvest Biol Technol,2007，47:136-140. [59]孙百灵,曲柏宏,李伟,等.苹果梨果实着色相关酶基因片段克隆及表达分析[J].吉林农业大学学报,2012,34(4)：423-427,442. [60]Ju Z，Duan Y.Effect of covering the orchard floor with reflecting films on pigment accumulation and fruit coloration in ‘Fuji’ apples[J].Sci Hort,1999a，82:47-56. [61]Jakopic J，Veberic R，Stampar F.The effect of reflective foil and hail nets on the lighting，color and anthocyanins of ‘Fuji’ apple[J].Sci Hort,2007，115:40-46. [62]Delong J M，Prange R K，Harrison P A，et al.The influence of crop-load，delayed cooling and storage atmosphere on post-storage quality of ‘Honeycrisp’ apples[J].J Hort Sci Biotechnol,2006，81:391-396. [63]Yonemori K.Japanese pomological magic：Producing fruits for gifts[J].Chron Hort,2009，49:15-18. [64]Lata B.Apple peel antioxidant status in relation to genotype，storage type and time[J].Sci Hort,2008，117:45-52. [65]McMeans O，Skirvin R M，Otterbacher A，et al.Assessment of tissue culture-derived ‘Gala’ and ‘Royal Gala’ apples (Malus domestica Borkh.) for somaclonal variation[J].Euphytica，1998，103:251-257. [66]Antonius-Klemola，Kalendar K R，Schulman A H.TRIM retrotransposons occur in apple and are polymorphic between varieties but not sports[J].Theor Appl Genet,2006,112：999-1008. [67]Hadanou A M，Gittins J R，Hiles E R，et al.Twoapple repetitive sequence elements：Characterisation and potential use as genetic markers[J].Euphytica，2003，131:177-187. [68]Han Y，Korban S S.Spring：A novel family of miniature inverted-repeat transposable elements is associated with genes in apple[J].Genomics，2007，90:195-200. [69]Cheng F S，Weeden N F，Brown S K.Identification of co-dominant RAPD markers tightly linked to fruit skin color in apple[J].Theor Appl Genet,1996，93:222-227. [70]Wakasa Y，Ishikawa R，Niizeki M，et al.Majin：A miniature DNA element associated with the genomes of pome fruit trees[J].Hortscience，2003，38:17-20. [71]Venturi S，Dondini L，Donini P，et al.Retrotransposon characterization and fingerprinting of apple clones by S-SAP markers[J].Theor Appl Genet,2005，112:440-444.
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备注/Memo
第一作者简介：孟富宣(1989-)，女，硕士研究生，现主要从事植物分子生物技术等研究工作。E-mail:616967560@qq.com. 责任作者：周军（1962-）,男，博士，教授，现主要从事果树分子生物学及林木遗传育种等研究工作。E-mail：zhoujunbo@163.com. 基金项目：国家林业局“948”资助项目(2013-4-44)；西南山地森林资源保育与利用省部共建教育部重点实验室开放基金资助项目(BC2010F08)；西南林业大学森林培育与经营教学团队建设经费资助项目(500961)；国家林业局重点实验室开放基金资助项目(BC2010F08)；云南省教育厅科学研究基金资助项目 (2012J050)。