[1]程振玉,宋海燕,杨英杰,等.AB-8大孔吸附树脂静态纯化北五味子木脂素工艺研究[J].北方园艺,2015,39(07):141-145.[doi:10.11937/bfyy.201507041]
　CHENG Zhen-yu,SONG Hai-yan,YANG Ying-jie,et al.Study on Technology of Static Purification of Lignans from Schisandra chinensis Baill by Macroporous Adsorption Resin[J].Northern Horticulture,2015,39(07):141-145.[doi:10.11937/bfyy.201507041]
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AB-8大孔吸附树脂静态纯化北五味子木脂素工艺研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年07 页码: 141-145 栏目: 出版日期: 2015-04-15
Title:
Study on Technology of Static Purification of Lignans from Schisandra chinensis Baill by Macroporous Adsorption Resin
作者:
程振玉1; 宋海燕2; 杨英杰1; 薛俊礼1; 吉惠杰1
（1.吉林化工学院 化学与制药工程学院，吉林 吉林 132022；2.吉林农业科技学院 实验中心，吉林 吉林 132101）
Author(s):
CHENG Zhen-yu1; SONG Hai-yan2; YANG Ying-jie1; XUE Jun-li1; JI Hui-jie1
（1.College of Chemical and Pharmaceutical Engineering，Jilin Institute of Chemical Technology，Jilin，Jilin 132022；2.Center of Experiments，Jilin Agricultural Science and Technology College，Jilin，Jilin 132101）
关键词:
高效液相色谱法; 北五味子; 木脂素; 大孔吸附树脂; 纯化
Keywords:
high performance liquid chromatography; Schisandra chinensis Baill; lignans; macroporous adsorption resin; purification
DOI:
10.11937/bfyy.201507041
文献标志码:
A
摘要:
以五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和五味子丙素为研究指标，以高效液相色谱作为检测方法，在考察不同类型大孔吸附树脂吸附和解析性能的基础上，对最佳树脂静态纯化北五味子木脂素的工艺进行探索。结果表明：AB-8大孔吸附树脂纯化效果最佳，最佳吸附条件为：提取液pH值为6.6，5种木脂素总浓度为161.815 mg/L，1 g大孔吸附树脂加入150 mL提取液，于55℃振荡吸附10 h；最佳解析条件为：以无水乙醇为解析剂，在65℃下解析8 h；在此条件下，5种木脂素的总含量由粗体物浸膏中的0.98%上升到5.86%。该方法为工业化大量生产北五味子木脂素提供了理论依据和数据支撑。
Abstract:
Taking Schisandrol A，Schisantherin A，Deoxyschisandrin，Schisandrin B and Schisandrin C as test index，the static purification conditions of lignans were determined on the basis of investigating the adsorption and desorption performance of different kind of macroporous resins.The results showed that,AB-8 macroporous resins was chose and employed in this study，the optimal adsorption conditions were as follows：adsorption sample volumes of 40 mL，pH value of 6.6，adsorption temperature of 55℃ and adsorption time of 10 hours；the optimal desorption conditions were desorption time of 8 hours，desorption temperature of 65℃，dehydrated ethanol as desorption solvent and the volume of 150 mL.Under these optimal conditions，the lignans content of Schisandra crude extracts increased from 0.98% to 5.86%.The method proposed in this study provided data and theoretical support.

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备注/Memo

第一作者简介：程振玉（1986-），男，硕士，助教，研究方向为天然产物化学。E-mail：844503608@qq.com. 责任作者：杨英杰（1955-），男，本科，教授，研究方向为天然产物化学。E-mail：yanghjm@163.com.

更新日期/Last Update: 2015-07-24