ZHANG Ji-tao,MA Da-wei.Research on Physiological Response to Water Stress in Tomato Seedling[J].Northern Horticulture,2015,39(07):46-49.[doi:10.11937/bfyy.201507015]
番茄幼苗对水分胁迫的生理响应
- Title:
- Research on Physiological Response to Water Stress in Tomato Seedling
- 文献标志码:
- A
- 摘要:
- 以醋栗番茄（‘L03708’）和栽培番茄（‘M82’）为试材，采用15%(w/v)浓度的PEG 6000模拟水分胁迫，研究了干旱敏感性不同的番茄幼苗随水分胁迫时间变化下的失水率、脯氨酸（Pro）含量、丙二醛（MDA）含量、过氧化氢（H2O2）含量以及超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性、抗坏血酸过氧化物酶（APX）的活性等指标的变化，以期进一步明确番茄幼苗对水分胁迫的生理效应。结果表明：在水分胁迫下，‘L03708’失水率显著低于‘M82’；在胁迫的1~48 h内，‘L03708’的Pro含量显著高于‘M82’；在胁迫1~72 h 内，‘L03708’的MDA、H2O2含量均显著低于‘M82’，‘L03708’的SOD、POD、CAT、APX活性均高于‘M82’。以上结果表明，番茄幼苗抗旱性强的原因在于能产生更多的渗透调节物质，维持渗透调节能力，增加吸水或保水能力，细胞抗氧化酶系统活性强，清除活性氧等物质，减少细胞膜的氧化反应。
- Abstract:
- To research the physiological effect of tomato to water stress，the contents of Pro，MDA and H2O2 and the activities of SOD,POD,CAT and APX of two different gene-type tomatoes，Solanum pimpinellifolium ‘L03708’ and Solanum lycopersicum ‘M82’ were examined，under Hoagland nutrient solution with 15% (w/v) PEG 6000.The results showed that the leaves of ‘L03708’ lost less water than leaves of ‘M82’；the content of Pro in ‘L03708’ was significantly higher than that in ‘M82’ during 1 hour to 48 hours；the contents of MDA and H2O2 in ‘L03708’ were lower than that in ‘M82’，while SOD，POD，CAT and APX activity were higher than that in ‘M82’ during 1 hour to 72 hours under water stress.The experiment results suggested that the drought tolerance of tomato seedling may due to the produce of more osmotic regulation substances which could maintain osmotic regulation ability，and more activity of antioxidant enzyme which could reduce the oxidation reaction of the cell membrane.
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备注/Memo
第一作者简介：张纪涛（1986-），男，硕士，现主要从事设施园艺工程等研究工作。E-mail：zhangjitao_xn@126.com. 基金项目：山西省农业科学院科技攻关资助项目（2010ygg25）；山西省科技攻关资助项目（20130311010-2）。