XIONG Yong,ZHAO Chun-yan,YANG Qing-song,et al.Molecular Cloning，Bioinformatics Analysis of rbcL Gene in Bellis perennis[J].Northern Horticulture,2015,39(04):89-95.[doi:10.11937/bfyy.201504021]
雏菊rbcL基因的克隆及其生物信息学分析
- Title:
- Molecular Cloning，Bioinformatics Analysis of rbcL Gene in Bellis perennis
- 文献标志码:
- A
- 摘要:
- 以雏菊为试验材料，采用PCR方法扩增雏菊rbcL基因并测序，并用DNAstar、PAML等生物软件对该基因进行生物信息学及其适应性进化研究。结果表明：雏菊rbcL基因序列全长1 508 bp，编码区全长1 458 bp，共编码485个氨基酸，与基因库中菊科其它属植物比较，核苷酸和氨基酸序列的同源性均超过了96%。rbcL蛋白二级结构主要有20个α螺旋、17个β折叠、33个转角以及一些无规则卷曲，适应性进化检测发现共有3个正选择氨基酸位点(94 E,149 Q,309 M)。获得完整雏菊rbcL基因有助于更深地研究雏菊对其生境的适应，rbcL蛋白大亚基正选择位点的空间结构对于维持核酮糖结构具有重要的作用。
- Abstract:
- Taking Bellis perennis as experimental materials.Bellis perennis rbcL gene was amplified using PCR method and sequenced.Biological softwares DNAstar，PAML was used to bioinformatic and adaptive evolutionary analysis for this gene.The results showed that the full length of cDNA of Bellis perennis rbcL gene was 1 458 bp，it encoded 485 amino acids.Compared to the sequences of this gene in other compositae plants，homology of their nucleotide and amino acid sequences were over 96%.The secondary structure of RbcL protein had 20 α-helices，17 β-sheets，33 turns and some coils structures.The adaptive evolutionary analysis using variable ω ratio sites method indicated that 3 amino acid residues(94 E，149 Q and 309 M) were positively selected.The isolation of rbcL gene will facilitate the further research in Bellis perennis adapting to particular environment.The spatial locations of the positively selected sites in rbcL subunit played an important role to maintain function of Rubisco.
参考文献/References:
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备注/Memo
第一作者简介：熊勇（1977-），男，布依族，硕士，副教授，现主要从事生物化学等研究工作。E-mail：17639121@qq.com.基金项目：国家自然科学基金资助项目（21262048）；云南民族大学大学生创新训练计划资助项目（0205-02010008）。