DUAN Hong-bin,QIAO Xin-rong,YIN Dong-lin,et al.Research Progress on Enhanced Cordycepin Production of Cordyceps militaris[J].Northern Horticulture,2015,39(02):178-181.[doi:10.11937/bfyy.201502050]
提高蛹虫草中的虫草素产量的研究进展
- Title:
- Research Progress on Enhanced Cordycepin Production of Cordyceps militaris
- Keywords:
- Cordyceps militaris; cordycepin; optimization
- 文献标志码:
- A
- 摘要:
- 虫草素是一种具有广泛生物活性和药理作用的核苷类物质，其药用价值及生物活性的多样性已众所周知。现从菌种选育、培养条件优化及提取工艺3方面综述了提高蛹虫草中虫草素产量的措施，以期对今后的研究方向进行展望。
- Abstract:
- Cordycepin is a member of nucleoside compounds which show extensive biological activity and pharmacological effects.Its medicinal value and biological activity has been widely accepted.Here some methods of improving the yield of cordycepin in Cordyceps militaris were reviewed，that including strain breeding，optimization of culture conditions，and extraction techniques.Additionally，future research directions were discussed.
参考文献/References:
[1]Sung G H，Hywel-Jones N L，Sung J M，et al.Phylogenetic classification of Cordyceps and the clavicipitaceous fungi[J]．Studies in Mycology，2007,57:5-59.[2]林晓民,李振岐,侯军.中国大型真菌多样性[M].北京:中国农业出版社,2004:38-39.[3]王建芳,杨春清.蛹虫草有效成分及药理作用研究进展[J].中医药信息,2005,22(5):30-32.[4]蒋宁,刘红锦,刘芳,等.虫草菌素研究与开发的现状与前景[J].江西农业学报,2011,23(1):121-123.[5]李文,赵世光,陈宏伟,等.低能离子束修饰蛹虫草菌株高产虫草素[J].生物工程学报,2009,25(11):1725-1731.[6]Das S K，Masuda M，Hatashita M,et al.A new approach for improving cordycepin productivity in surface liquid culture of Cordyceps militaris using high-energy ion beam irradiation[J].Letters in Applied Microbiology，2008,47(6):534-538.[7]周洪英,边银丙.蛹虫草虫草素高产原生质体融合子鉴定与筛选[J].食用菌学报,2007,14(2):65-70.[8]周礼红,王仕敏,罗丽美.高产虫草菌素蛹虫草菌株的紫外线诱变选育[J].食用菌,2009(4):20-21.[9]周晓东,李明,田景花,等.北虫草菌丝体诱变及优良菌株的筛选[J].北方园艺,2013(8):154-156.[10]温鲁,张诚,夏敏,等.蛹虫草航天搭载对活性成分含量的影响[J].食品科学,2008,29(5):382-384.[11]Mao X B,Eksriwong T,Chauvatcharin S,et al.Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris [J].Process Biochemistry,2005,40(5):1667-1672.[12]Shih I L,Tsai K L,Hsieh C,et al.Effects of culture conditions on the mycelial growth and bioactive metabolite p roduction in submerged culture of Cordyceps militaris [J].Biochemical Engineering Journal,2007,33(3):193-201. [13]顾冬艳,尹永祺,杨润强,等.北虫草固态发酵产虫草素培养基优化[J].食品科学,2013,34(21):254-258.[14]蔡水淋,郑永标,王明兹,等.响应面法优化蛹虫草深层培养产菌丝及虫草素的发酵工艺[J].农产品加工,2013,311(3):13-18.[15]钟思敏,杜梅,陈往滨,等.蛹虫草菌丝产虫草素液体培养条件的研究[J].菌物学报，2011,30(2):229-234.[16]施渺筱,舒星,肖洋,等.正交实验优化蛹虫草培养成分提高虫草菌素含量[J].时珍国医国药,2009,20(9):2221-2222.[17]温鲁,夏敏,葛宜和,等.以虫草素和腺苷含量为指标优化蛹虫草人工栽培[J].江苏农业学报，2005,21(4)：359-363.[18]温鲁,夏敏,宋虎卫,等.液体培养蛹虫草虫草素和腺苷的代谢量[J].微生物学通报,2005,232(3):91-94.[19]韦会平,叶小莉,张华英,等.用蛹虫草固体发酵法高效生产虫草素的研究[J].中国中药杂志,2008,33(19)：2159-2162.[20]叶晶晶,张剑飞,曹宁宁,等.添加蚕蛹粉和改变营养液pH 对人工培养蛹虫草子实体产量及虫草素含量的影响[J].蚕业科学,2013,9(3):624-627.[21]郭涛,张龙,王兵,等.不同培养基培育蛹虫草中的虫草素和腺苷含量测定[J].蚕业科学,2011,37(6):1117-1122.[22]荆留萍,杜双田,金凌云.8种物质对蛹虫草液体发酵中虫草素及多糖含量的影响[J].西北农林科技大学学报（自然科学版）,2010,38(11):156-160.[23]Masuda M,Urabe E,Honda H,et al.Enhanced production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris[J].Enzyme and Microbial Technology,2007,40(5):1199-1205.[24]陈长兰,孟程程,佟丽.不同种类氨基酸对蛹虫草菌丝体生长和虫草素含量的影响[J].食品科学,2012,33(23):236-239.[25]左言美,程显好,朱萌,等.锰离子胁迫对蛹虫草菌丝体内虫草素、腺苷含量的影响[J].中国药学杂志,2013,48(16):1363-1368.[26]文庭池,康冀川,李光荣,等.固体培养条件对蛹虫草产子实体和虫草菌素的影响[J].贵州农业科学,2008,36(4):92-94.[27]周毅峰,蔡忠雄,秦恩华,等.硒对蛹虫草菌丝代谢和总抗氧化能力的影响[J].中国酿造,2009(4):48-51.[28]Mao X B，Zhong J J.Significant effect of NH4+on cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris[J].Enzym and Microbial Technology,2006,38(3):343-350.[29]肖正华,李再新,李建章,等.添加剂对蛹虫草子实体生长分化影响的研究[J].食品与发酵科技,2010,46(3):60-64.[30]施明珠,李有贵,钟石,等.保幼激素III对蛹虫草液体发酵生长代谢的调控作用[J].科技通报,2009,25(1):114-119.[31]康超,文庭池,康冀川,等.不同培养条件和前体对蛹虫草液体发酵产虫草素的影响[J].菌物学报,2012,31(3):389-397.[32]柳依婷,汤佳鹏,周成,等.甘氨酸补加产虫草素的蛹虫草液体发酵条件的优化[J].食品科技,2013,38(7):28-32.[33]Mao X B,Zhong J J.Hyperproduction of cordycepin by two-stage dissolved oxygen control in submerged cultivation of medicinal mushroom Cordyceps militaris in bioreactors[J].Biotechnology Progress,2004,20(5):1408-1413.[34]赵博,张国财,林连男,等.生物磁效应对蛹虫草活性物质含量的影响[J].植物研究,2013,33(4):508-511.[35]王陶,李文,陈宏伟,等.虫草素的微波-超声波协同提取[J].食品科学,2010,31(10):86-90.[36]李辰,吴盼盼,卿宁,等.蛹虫草废弃培养残基中虫草素的提取工艺研究[J].五邑大学学报（自然科学版）,2012,26(3):35-41.[37]邓黎,周同永,皮立,等.用响应面法优化人工蛹虫草子实体中虫草素的超声提取工艺[J].江苏农业科学,2012,40(5):225-228.[38]谢红旗,胡瑕,罗巍,等.酶法提取蛹虫草中虫草素的研究[J].时珍国医国药,2011,22(9):2145-2147.[39]李从镇,毛宁.大孔吸附树脂分离纯化培养基残基中虫草素[J].中国生物工程杂志,2014,34(1):90-94.[40]曾昱,陈作红.大孔树脂柱层析法提取蛹虫草废弃培养基中的虫草素[J].湖南师范大学自然科学学报,2013,36(3):66-69.
相似文献/References:
[1]孙露露,李焕端,张至远,等.蛹虫草甜米酒的生产工艺研究[J].北方园艺,2014,38(05):125.
SUN Lu-lu,LI Huan-duan,ZHANG Zhi-yuan,et al.Study on Production Techniques of Cordyceps Militaris Sweet Rice Wine[J].Northern Horticulture,2014,38(02):125.
[2]方华舟.蛹虫草菌种培养性状与出草结果关系的研究[J].北方园艺,2013,37(12):161.
FANG Hua-zhou.Study on the Relationship Between Cultural Characteristics and Results of Weeds of Cordyceps militaris[J].Northern Horticulture,2013,37(02):161.
[3]殷东林,段鸿斌,王锐丽.蛹虫草有性生殖研究[J].北方园艺,2014,38(03):130.
YIN Dong-lin,DUAN Hong-bin,WANG Rui-li.Study on the Sexual Reproduction of Cordyceps militaris[J].Northern Horticulture,2014,38(02):130.
[4]周晓东,李明,田景花,等.北虫草菌丝体诱变及优良菌株的筛选[J].北方园艺,2013,37(08):154.
ZHOU Xiao-dong,LI Ming,TIAN Jing-hua,et al.Mutagenesis of Mycelial of Cordyceps militaris and Selection of Fine Strains[J].Northern Horticulture,2013,37(02):154.
[5]暴增海,贾超艳,江倩倩,等.真姬菇、鸡汁菇和蛹虫草培养特性的观察与生长量的测定[J].北方园艺,2012,36(23):162.
BAO Zeng-hai,JIA Chao-yan,JIANG Qian-qian,et al.Cultivation Characteristics and Growth of Hypsizygus marmoreus，Jizhigu and Cordyceps militaris[J].Northern Horticulture,2012,36(02):162.
[6]郑素月,郝俊灵,王建伟.继代培养对蛹虫草菌丝生长速度及酯酶同工酶的影响[J].北方园艺,2012,36(10):136.
ZHENG Su-yue,HAO Jun-ling,WANG Jian-wei.The Influences of Subculture of Cordceps militaris to Mycelial Growth Rate and Esterase Isozyme[J].Northern Horticulture,2012,36(02):136.
[7]方华舟.不同蛹虫草优质母种分离方式与菌种性能比较[J].北方园艺,2014,38(15):142.
FANG Hua-zhou.Comparison of Separation Method and Performance Comparison Among Different High Quality Stock Culture of Cordyceps militaris[J].Northern Horticulture,2014,38(02):142.
[8]林群英,吴亮亮,张锋伦,等.蛹虫草栽培过程中胞外蛋白酶活性变化研究[J].北方园艺,2014,38(17):148.
LIN Qun-ying,WU Liang-liang,ZHANG Feng-lu,et al.Study on Extracellular Protease Activity Change During Fruitbody Growth of Cordyceps militaris[J].Northern Horticulture,2014,38(02):148.
[9]左雪枝,朱运英,方华舟.不同光照强度对蛹虫草菌丝及子实体生长的影响[J].北方园艺,2015,39(16):140.[doi:10.11937/bfyy.201516033]
ZUO Xuezhi,ZHU Yunying,FANG Huazhou.Effect of Different Light Intensities on the Growth of Hyphal and Sporocarp in Cordyceps militaris[J].Northern Horticulture,2015,39(02):140.[doi:10.11937/bfyy.201516033]
[10]秦 秀 丽,杨 国 会,李 风 林.蛹虫草液体深层发酵的研究[J].北方园艺,2010,34(23):0.[doi:10.11937/bfyy.201023065]
QIN Xiu -li,YANG Guo -hui,LI Feng -lin.Submerged Fermentation of Cordycepsmilitaris[J].Northern Horticulture,2010,34(02):0.[doi:10.11937/bfyy.201023065]
[11]刘 洋,孙来玉,陆云华,等.九株蛹虫草菌株的生物学性状研究[J].北方园艺,2013,37(20):140.
LIU Yang,SUN Lai-yu,LU Yun-hua,et al.Study on the Biological Characteristics of Nine Cordyceps militaris Strains[J].Northern Horticulture,2013,37(02):140.
[12]付彦凯,冷云伟,曹文化.蛹虫草的人工培育历程[J].北方园艺,2012,36(04):178.
FU Yan-kai,LENG Yun-wei,CAO Wen-hua.Artificial Cultivation Progress of Cordyceps militaris[J].Northern Horticulture,2012,36(02):178.
[13]秦鹏,王龙,路等学,等.响应面法优化蛹虫草菌液体发酵条件[J].北方园艺,2016,40(08):138.[doi:10.11937/bfyy.201608038]
QIN Peng,WANG Long,LU Dengxue,et al.Optimization of Fermentation Conditions Mycelium Production by Cordyceps militaris Using Response Surface Methodology[J].Northern Horticulture,2016,40(02):138.[doi:10.11937/bfyy.201608038]
[14]简利茹,杜双田.氮源和无机盐对蛹虫草子座产量及虫草素和腺苷的影响[J].北方园艺,2016,40(19):163.[doi:10.11937/bfyy.201619041]
JIAN Liru,DU Shuangtian.Effect of Nitrogen and Inorganic Salt on Production of Stroma，Cordycepin and Adenosine in Cordyceps militaris[J].Northern Horticulture,2016,40(02):163.[doi:10.11937/bfyy.201619041]
[15]李居宁,方华舟.不同栽培温度对蛹虫草主要活性成分的影响[J].北方园艺,2017,41(02):157.[doi:10.11937/bfyy.201702038]
LI Juning,FANG Huazhou.Variations of Key Active Ingredients of Cordyceps militari Under Different Temperatures[J].Northern Horticulture,2017,41(02):157.[doi:10.11937/bfyy.201702038]
[16]张楠,黎勇,徐洁,等.蛹虫草深层发酵产虫草素培养基的优化[J].北方园艺,2017,41(05):134.[doi:10.11937/bfyy.201705032]
ZHANG Nan,LI Yong,XU Jie,et al.Medium Optimization for Cordycepin Production From Cordyceps militaris by Submerged Fermentation[J].Northern Horticulture,2017,41(02):134.[doi:10.11937/bfyy.201705032]
[17]李晓薇,张敏,沈丽君,等.蛹虫草液体发酵过程中虫草素含量的分析[J].北方园艺,2017,41(19):170.[doi:10.11937/bfyy.20170907]
LI Xiaowei,ZHANG Min,SHEN Lijun,et al.Analysis of Content of Cordycepin in Liquid Fermentation of Cordyceps militaris[J].Northern Horticulture,2017,41(02):170.[doi:10.11937/bfyy.20170907]
[18]方华舟,肖习明.不同光照强度对蛹虫草 主要活性成分的影响规律[J].北方园艺,2017,41(24):170.[doi:10.11937/bfyy.20171650]
FANG Huazhou,XIAO Ximing.Influence of Light Intensity on the Key Active Ingredients of Cordyceps militaris[J].Northern Horticulture,2017,41(02):170.[doi:10.11937/bfyy.20171650]
[19]王晓玉,于金艳,罗佳豪,等.基于氧化应激反应提高蛹虫草中虫草素产量的激发子筛选及发酵条件优化[J].北方园艺,2022,(16):113.[doi:10.11937/bfyy.20220433]
WANG Xiaoyu,YU Jinyan,LUO Jiahao,et al.Screening of Elicitors and Optimization of Fermentation Conditions for Enhancing Cordycepin Production in Cordyceps militaris Based on Oxidative Stress[J].Northern Horticulture,2022,(02):113.[doi:10.11937/bfyy.20220433]
备注/Memo
第一作者简介：段鸿斌（1970-），男，河南罗山人，硕士，副教授，现主要从事生物技术等研究工作。E-mail:dhb19700102@163.com.基金项目：河南省科技攻关资助项目(132102110047)。