LI Fan-hai,WANG Gui-qing.The Antibacterial Activity of Six Kinds Violet Magnolia Parallel Extracts on Bocrytis cinerea Pers.[J].Northern Horticulture,2015,39(02):108-111.[doi:10.11937/bfyy.201502030]
辛夷六种溶剂平行提取物对黄瓜灰霉病菌的抑制作用
- Title:
- The Antibacterial Activity of Six Kinds Violet Magnolia Parallel Extracts on Bocrytis cinerea Pers.
- 文献标志码:
- A
- 摘要:
- 采用平行提取法,研究石油醚、氯仿、乙酸乙酯、丙酮、乙醇、水6种溶剂对辛夷提取率的影响,采用生长速率法和孢子萌发法分别测定了辛夷6种平行提取物对黄瓜灰霉病致病菌——灰葡萄孢菌的菌丝生长和孢子萌发的抑制效果。结果表明:去离子水的提取率最大为25.71%,石油醚最小为9.63%;辛夷6种平行提取物对灰葡萄孢菌的菌丝生长和孢子萌发均有抑制作用;石油醚、氯仿提取物对菌丝生长的EC50分别为372.15、206.83 mg/L,对孢子萌发的EC50分别为1 318.92、591.78 mg/L,抑菌效果较好;水提取物对菌丝生长和孢子萌发的EC50分别为6 024.25、3 532.13 mg/L。说明辛夷有机溶剂提取物对菌丝生长的抑制效果优于其对孢子萌发的抑制效果,且辛夷的主要抑菌活性成分较易被极性小的有机溶剂提取。
- Abstract:
- The effects on violet magnolia extraction ratio of petroleum ether,chloroform,ethyl acetate,acetone,ethanol,deionized water were studied with parallel extract methods.The inhibitory effects of 6 kinds violet magnolia parallel extracts on hyphae growth and spore germination of Bocrytis cinerea Pers. pathogen were studied with growth rate and spores germination methods.The results showed that the extracted rate of deionized water was the largest,which was 25.71%.The smallest was petroleum ether which was 9.63%.6 kinds solvent parallel extracts had significant antibacterial effects on hyphae growth and spore germination of pathogen.The EC50 of petroleum ether,chloroform and deionized water extracts on hyphae growth and spore germination was 372.15 mg/L and 1 318.92 mg/L,206.83 mg/L and 591.78 mg/L,6 024.25 mg/L and 3 532.13 mg/L respectively.Indicate that the inhibition effect on hyphae growth was superior to spore germination of organic solvents extracts.And the main inhibiting activity substance of violet magnolia could be extracted by polar organic solvents easily.
参考文献/References:
[1]屠豫钦.正确认识化学农药的问题[J].植物保护,2003,29(4):11-15.
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备注/Memo
第一作者简介:李凡海(1988-),男,山东聊城人,硕士研究生,研究方向为有害生物防治。E-mail:weixiaodexiwang@126.com.责任作者:王桂清(1968-),女,河北泊头人,博士,教授,现主要从事植物保护的教学与科研工作。E-mail:wangguiqing@lcu.edu.cn.基金项目:山东省自然科学基金资助项目(ZR2012CL17);聊城大学重点建设资助项目(13KZ0801)。