CHEN Yong-gan,CHEN Guang-zhou,YUAN Xue-jun,et al.Phylogenetic Analysis and Cultivation Technique of Ganodermalucidum?from China[J].Northern Horticulture,2014,38(08):92-96.
中国产Ganoderma lucidum的系统发育学分析及栽培技术研究
- Title:
- Phylogenetic Analysis and Cultivation Technique of Ganodermalucidum?from China
- 文章编号:
- 1001-0009(2014)08-0092-05
- 关键词:
- 系统发育学; rDNA-ITS; mt SSU; Ganoderma lucidum
- Keywords:
- phylogenetics; rDNA-ITS; mt SSU; Ganoderma lucidum
- 分类号:
- S 567.3+1
- 文献标志码:
- A
- 摘要:
- 灵芝是一类木腐真菌，广泛存在于热带雨林中。根据形态特征、rDNA-ITS和mt SSU的系统发育学分析，课题组将从Cyclobalanopsis bambusaefolia分离出来的菌株命名为G.lucidum，该菌株可人工培养。系统发育学分析表明，这些菌株类群与亚洲灵芝为一个独立的分支；此外，该研究采用传统椴木栽培技术，实现了分离菌株的人工栽培。人工栽培灵芝子实体粗厚，木栓质，边缘淡黄，在成熟的过程中逐渐变褐。
- Abstract:
- Ganoderma strains are wood-rotting fungi and popular living in tropical forest.Based on the morphological characteristics，phylogenetics analysis of rDNA-ITS and mitochondrial small-subunit ribosomal DNA sequences，we proposed isolates collected from Cyclobalanopsis bambusaefolia grown in China were G.lucidum，also this group isolates could be artificial cultivation.Morphology，host distribution and molecular phylogenetic evidence supported recognition of this species.Phylogenetic analysis indicated these isolates clustered with Asian G.lucidum into a distinct clade.Artificial cultivation showed fruiting bodies were thick，corky，and yellowish in the growing margins and then turn to brownish in the matured part on the surface.
参考文献/References:
[1]Shiao M S.Natural products of the medicinal fungus Ganoderma lucidum :occurrence ， biological activities ， and pharmacological functions[J].Chemical Record ， 2003(3):172-180. [2]Boh B M，Berovic J S，Lin Z B.Ganoderma lucidum?and its pharmaceutically active compounds[J].Biotechnology Annual Review，2007，13:265-301. [3]Hong S G，Jung H S.Phylogenetic analysis of Ganoderma?based on nearly complete mitochondrial small-subunit ribosomal DNA sequences[J].Mycologia，2004，96:742-755. [4]Meli C D，Langer E.Ganoderma carocalcareussp.nov.，with crumbly-friable context parasite to saprobe on Anthocleista nobilisand its phylogenetic relationship in G.resinaceum group[J].Mycological Progress，2009(8):145-155. [5]Gottlieb A M，Ferrer E，Wright J E.rDNA analyses as an aid to the taxonomy of species of Ganoderma[J].Mycological Research，2000，104:1033-1045. [6]Wu X L，Dai Y C.Illustration handbook of Ganoderma genus[M].Beijing:Science Press，2004:1-14.(in Chinese) [7]Wang D M，Yao Y J.Intrastrain internal transcribed spacer heterogeneity in Ganoderma?species [J].Canadian Journal of Microbiology，2005，51:113-121. [8]Sun S J，Gao W，Lin S Q，et al.Analysis of genetic diversity in Ganoderma?population with a novel molecular marker SRAP [J].Applied Microbiology and Biotechnolog，2006，72:537-543. [9]Guglielmo F，Gonthier P，Garbelotto M，et al.A PCR-based method for the identification of important wood rotting fungal taxa within Ganoderma，Inonotus s.l.and Phellinus?s.l [J].FEMS Microbiology Letters，2008，282:228-237. [10]Li W，Ji Y L，Yu H S，et al.A new species of Epichlo?symbiotic with Chinese grasses [J].Mycologia，2006，98:560-570. [11]White T J，Bruns T，Lee S，et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetic.In:Innis M A，Gelfand D H，Sninsky J J，White T J (eds) PCR protocols:A guide to methods and applications[M].New York:Academic Press，1990:315-322. [12]Patterton H G，Graves S.DNAssist:the integrated editing and analysis of molecular biology sequences in windows[J].Bioinformatics，2000，16:652-653. [13]Thompson J D，Higgins D G，Gibson T J.CLUSTAL W:improving the sensitivity of progressive multiple sequence alignment through sequence weighting，position-specific gap penalties and weight matrix choice[J].Nucleic acids research，1994，22(22):4673-4680. [14]TamuraK，Dudley J，Nei M，et al.MEGA4:Molecular Evolutionary Genetics?Analysis (MEGA) software version 40[J].Molecular Biology and Evolution，2007，24:1596-1599. [15]Mayzumi F，Okamoto H，Mizuno T.Cultivation of Reishi (Ganoderma lucidum)[J].Food Reviews International，1997，13:365-382. [16]Chen A W.Cultivation of the medicinal mushroom Ganoderma lucidum (Curt.:Fr.) P.Karst.(Reishi) in North America[J].International Journal of Medicinal Mushrooms，1999(1):207-215. [17]Stamets P.Growing Gourmet and Medicinal Mushrooms[M].Berkeley:Ten Speed Press，2000:352-366. [18]Chang S T，Miles P G.Mushrooms cultivation，nutritional value，medicinal effect，and environmental impact[M].Florida:CRC Press，2004:357-372. [19]Chen A W，Miles P G.Cultivation of Ganoderma?Bonsai[M].Mushroom Biology and Mushroom Products，1996:325-333. [20]Yang F C，Hsieh C Y，Chen H M.Use of stillage grain from a rice-spirit distillery in the solid state fermentation of Ganoderma lucidum[J].Process Biochemistry，2003，39:21-26. [21]Zheng L Y，Jia D H，Fei X F，et al.An assessment of the genetic diversity within Ganoderma?strains with AFLP and ITS PCR-RFLP[J].Mycological Progress，2009，164:312-321.
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备注/Memo
第一作者简介：陈永敢（1985-），男，海南乐东人，硕士，研究方向为微生物进化。E-mail:yonggan2006@163.com.