QIN Ping-sheng,ZHANG Yu,HOU Li-xia,et al.Effect of Citrus grandis var.shatinyu Hort.Style Protein on Its Pollen Germination and Growth[J].Northern Horticulture,2014,38(03):88-91.
沙田柚花柱蛋白对其花粉萌发及生长的影响
- Title:
- Effect of Citrus grandis var.shatinyu Hort.Style Protein on Its Pollen Germination and Growth
- 文章编号:
- 1001-0009(2014)03-0088-04
- Keywords:
- Citrus grandis var.shatinyu Hort.; pollen germination; pollen tube length; style proteins; Gel electrophoresis
- 分类号:
- S 666.3
- 文献标志码:
- A
- 摘要:
- 以沙田柚花粉为试材，研究比较了自交和异交花柱提取蛋白对花粉萌发和花粉管生长的影响。结果表明：添加沙田柚自交、异交花柱蛋白后，花粉的萌发未受影响。但花粉管的生长情况在二者之间有明显差异，添加异交花柱蛋白对花粉管的生长没有影响，而添加自交花柱蛋白的花粉管的生长受到明显抑制。PAGE电泳显示自交花柱蛋白处理的花粉管出现了1条特异蛋白带（Rf值=0.81）；SDS-PAGE电泳检测到自交花柱蛋白处理的花粉管出现了相对分子量为35.0 kD和37.5 kD的2条特异蛋白带；IEF-PAGE电泳的结果表明，在自交花柱蛋白处理的花粉管蛋白中出现了等电点为5.85和6.20的2条的特异蛋白谱带。
- Abstract:
- Taking the pollen of Citrus grandis var.shatinyu Hort. as material，effect of the self-pollinated style proteins and the cross-pollinated style proteins on pollen germination and pollen tube growth were compared.The results showed that pollen germination was unaffected by adding self-pollinated style protein and the cross-pollinated style protein,but significantly affected the growth of pollen tube.The growth of pollen tube in those media with the self-pollinated style proteins was restrained,with cross-pollinated style proteins was unaffected.PAGE electrophoresis showed that the pollen tube self style protein processing of the 1 specific protein bands (Rf=0.81)；tube SDS-PAGE electrophoresis to self styleprotein processing appeared relative molecular weight of 2 specific protein of 35 kD and 37.5 kD band；IEF-PAGE electrophoresis results showed that，in the self style protein treated pollen tube protein in the isoelectric points of 5.85 and 6.20 of the two specificprotein bands.
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备注/Memo
第一作者简介：覃屏生(1976-)，男，广西环江人，硕士，讲师，现主要从事植物分子生物学等研究工作。E-mail：qps@mailbox.gxnu.edu.cn.