WANG Xi,LIU Jia,BAO Zeng-hai,et al.Study on Promoting Ability and Induced Resistance of Marine Paenibacillus polymyxa Strain L1-9 in Cucumber[J].Northern Horticulture,2014,38(21):133-137.
海洋多粘类芽孢杆菌L1-9菌株对黄瓜的促生作用和诱导抗性研究
- Title:
- Study on Promoting Ability and Induced Resistance of Marine Paenibacillus polymyxa Strain L1-9 in Cucumber
- 文章编号:
- 1001-0009（2014）21-0133-05
- 关键词:
- 多粘类芽孢杆菌L1-9菌株; 促生作用; 诱导抗性
- 分类号:
- S 642.204+.1
- 文献标志码:
- A
- 摘要:
- 以黄瓜种子为试材，采用盆栽法研究了L1-9菌株发酵液浸泡黄瓜种子后，对黄瓜幼苗的根长、须根数、黄瓜枯萎病发生的影响，并测定了黄瓜根组织的过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）、超氧化物歧化酶（SOD）和多酚氧化酶（PPO）4种防御酶的酶活性变化。结果表明：L1-9菌株发酵液浸泡黄瓜种子，可促进黄瓜须根的增加和根伸长，对黄瓜枯萎病具有明显的防治效果；浸种处理后黄瓜幼苗根部PAL、POD、PPO、SOD 4种酶活性明显高于对照，4种酶活性高峰时期不同，表明L1-9菌株发酵液浸泡种子能够明显诱导黄瓜对枯萎病的抗性。
- Abstract:
- Taking Paenibacillus polymyxa as material,the promoting ability and disease prevention effect of L1-9 on cucumber were studied under the treatment with soaking seeds by the Oxford cup method.The length of cucumber seedlings root,the number of fibrous roots and the inhibition of fusarium wilt of cucumber were measured.At the same time,four defense enzymes in cucumber roots as phenylalaninaminolyase(PAL),peroxidase(POD),polyphenoloxidas(PPO) and superoxide dismutase(SOD) were studied.The results showed that L1-9 fermentation broth could promote the increase of fibrous roots and elongation of root,and had an obvious effect on fusarium wilt of cucumber;the activity of PAL,POD,PPO and SOD in cucumber seedlings roots after soaking treatments was significantly higher than control treatment.These demonstrated that strain L1-9 could induce cucumber’s resistance to fusarium wilt under the treatment as soaking seeds.
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备注/Memo
第一作者简介：王希（1993-），女，江苏苏州人，本科，研究方向为海洋微生物利用。E-mail：1374457257@qq.com.
责任作者：暴增海（1962-），男，河北沧州人，硕士，教授，现主要从事植物病害生物防治等研究工作。E-mail：baozh2008@aliyun.com.
基金项目：江苏省高校自然科学资助项目（12KJA210001）；江苏省农业科技支撑资助项目(BE2012375)；连云港市科技局农业攻关资助项目（CN1207）；国家级大学生实践创新训练计划资助项目（G201411641105002）；江苏省“十二五”高等学校水产类重点专业资助项目。
收稿日期：2014-08-10