GUO Xin-xin,FAN Shu-hua,WANG Yong-li.Study on the Optimization of Liquid Cultural Medium of Flammulina velutipes [J].Northern Horticulture,2014,38(02):146-148.
金针菇液体培养基的优化研究
- Title:
- Study on the Optimization of Liquid Cultural Medium of Flammulina velutipes
- 文章编号:
- 1001-0009(2014)02-0146-03
- Keywords:
- Flammulina velutipes; liquid strain; culture medium; optimization
- 分类号:
- S 646
- 文献标志码:
- A
- 摘要:
- 以金针菇为试材，以菌丝体干重和菌丝球数目为考察指标，选择碳源、氮源、无机盐、维生素为影响因素，通过发酵试验和正交实验确定最佳液体种子培养基。结果表明：金针菇液体培养的最佳碳源为可溶性淀粉，最佳氮源为黄豆粉，最佳无机盐为KH2PO4，最佳维生素为VB2；最佳培养基配方为4%可溶性淀粉、5%黄豆粉、0. 05%KH2PO4、0. 0075% VB2。
- Abstract:
- Taking Flammulina velutipes as material，the factors influencing mycelium growth were screened in order to determine the best liquid strain culture medium of Flammulina velutipes that produce intracellular polysacchride.The dry weight of the mycelia and the number of mycelium pellet were selected as the investigation index for determining the carbon source，nitrogen source，inorganic salt and vitamin.The fermentation test and the orthogonal test were used to analysis the four factors and to determine the optimum culture medium of liquid strain of F.velutipes.The results showed that the optimum carbon source for liquid culture of F.velutipes was soluble starch，the optimum nitrogen source was soybean flour，the optimum inorganic salt was KH2PO4，and the optimum vitamin was VB2，and the optimum formula of culture medium was as follows：4% soluble starch，5% soybean flour，0. 05% KH2PO4，0. 0075% VB2.
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备注/Memo
第一作者简介：郭欣欣（1989-），女，本科，研究方向为生物技术。