WEI Zhi-ying,SHA Wei,ZHANG Mei-juan.Study on Tissue Culture Conditions of Tortula ruralis[J].Northern Horticulture,2014,38(18):114-117.
山墙藓组织培养条件研究
- Title:
- Study on Tissue Culture Conditions of Tortula ruralis
- 文章编号:
- 1001-0009（2014）18-0114-04〖HK〗
- 分类号:
- Q 949.35+2
- 文献标志码:
- A
- 摘要:
- 以山墙藓幼嫩茎尖为外植体，采用L12(3×24)正交设计方法，对山墙藓配子体消毒方法及培养基类型进行了筛选；通过梯度设计研究不同浓度的蔗糖对配子体形态发生的影响，以建立有效的山墙藓（Tortula ruralis）组织培养快繁体系。结果表明：山墙藓配子体的最佳消毒方法为浓度3%的次氯酸钠溶液消毒5 min后，浓度0.05%的升汞溶液二次消毒20 s，Beneck培养基为最适消毒培养基；蔗糖对于山墙藓原丝体的形成和扩展生长均具促进作用，浓度为30 g/L时原丝体形成时间最早，扩展直径最大，但抑制了芽体的分化，浓度为15 g/L时对芽体分化具明显促进作用。
- Abstract:
- Taking apical shoots of Tortula ruralis as the explant，adopting L12(3×24) orthogonal design method，the disinfection method and medium type were chosen in in virto culture,the effect of different sucrose concentrations on gametophyte morphogenesis were investigated through the gradient design in order to establish effective propagation system of Tortula ruralis.The results showed that the best way of T.ruralis gametophyte sterilization was as follows:3% Sodium hypochlorite disinfecting for 5 minutes，then 0.05% Mercuric chloride disinfecting for 20 seconds，as a basal medium for establishment of in vitro culture，Beneck was the appropriate medium.The sucrose concentrations tended to have a positive effect on protonema formation and elongation，when 30 g/L sucrose was added in the medium，protonema form at the earliest time and extend the largest diameter，but it inhibited buds formation，when 15 g/L sucrose was added in the medium，it obviously promoted buds formation.
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备注/Memo
第一作者简介：魏志颖（1983-），女，硕士研究生，研究方向为苔藓植物生物学。E-mail：wzy19830515@163.com.
责任作者：沙伟(1963-)，女，博士，教授，博士生导师，研究方向为苔藓植物遗传学和分子生物学。E-mail：shw1129@263.net.
基金项目：国家自然科学基金资助项目(31070180；31270254)。
收稿日期：2014-05-05