[1]李小梅,张立微,张景涛.芫荽遗传多样性RAPD分析[J].北方园艺,2014,38(15):97-100.
　LI Xiao-mei,ZHANG Li-wei,ZHANG Jing-tao.RAPD Analysis of Genetic Diversity for Coriander[J].Northern Horticulture,2014,38(15):97-100.
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芫荽遗传多样性RAPD分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年15期 页码: 97-100 栏目: 出版日期: 2014-08-15
Title:
RAPD Analysis of Genetic Diversity for Coriander
文章编号:
1001-0009(2014)15-0097-04
作者:
李小梅; 张立微; 张景涛
(哈尔滨市农业科学院 蔬菜花卉分院,黑龙江 哈尔滨 150029
Author(s):
LI Xiao-mei; ZHANG Li-wei; ZHANG Jing-tao
Vegetables and Flowers Research Institute,Harbin Academy of Agricultural Sciences,Harbin,Heilongjiang 150029
关键词:
芫荽; RAPD; 遗传多样性
Keywords:
Coriandrum sativum; RAPD; genetic diversity
分类号:
S573+.2
文献标志码:
A
摘要:
以96份芫荽为试材，采用RAPD分析方法，研究了芫荽种质资源遗传多样性。结果表明：从300条随机引物中筛出23条扩增性强、清晰度高和稳定性好的引物，扩增出53条带，其中34条为多态性带，多态性比例64.2%。通过聚类分析，生成96份芫荽亲缘关系树状图，在遗传距离0.50处，试材划分为6类。RAPD分析揭示了芫荽的遗传背景并为其分类提供了依据。
Abstract:
Taking 96 coriander samples as materials,using the RAPD analysis method,the genetic diversity of germplasm resources of coriander was studied.The results showed that 23 random primers were screened from 300 primers that were amplification of strong,high resolution and good stability.34 bands were polymorphic among 53 bands identified.The percentage of polymorphic loci was 64.2%.All the germplasm could be divided into six categories at the genetic distance 0.50 by cluster analysis.RAPD analysis reflected the complex genetic background and provided the supporting to the identification and classiflcation of coriander.

参考文献/References:

［1］郭红转,陆占国,李健.芫荽的研究开发现状［J］.食品研究与开发,2005,26(2):104-106.

［2］李良松,刘懿,杨丽萍.香药本草［M］.北京:中国医药科技出版社,2000.[JP3]
［3］唐庭栎.大兴安岭药用资源［M］.哈尔滨:哈尔滨出版社,2001.
［4］黄三文,王晓武,张宝玺,等 蔬菜作物分子标记研究进展与我国的发展策略［J］.中国蔬菜,1999(1)：50-53.
［5］张应华,刘荣,许彬.分子标记在蔬菜作物上的应用［J］.云南农业大学学报,2002,17(3)：287-290.
［6］季祥彪,王国鼎,乔光,等.贵州野生春兰遗传多样性的RAPD 分析［J］.华中农业大学学报,2008,27(2):297-302.
［7］Lo′pez P A,Widrlechner M P,Simon P W,et al.Assessing phenotypic,biochemical,and molecular diversity in coriander (Coriandrum sativumL.) germplasm［J］.Genet Resour Crop Evol,2008,55:247-275.
［8］马晓静,李广平,郑晓静,等.20个青菜品种的遗传多样性分析［J］.长江蔬菜(学术版),2012(22):13-16.
［9］高日,廉美兰,吴松权,等.东北刺人参遗传多样性的RAPD分析［J］.湖北农业科学,2008,47(6):627-628.
［10］华树妹,涂前程,雷伏贵.福建山药种质资源遗传多样性的 RAPD分析［J］.植物遗传资源学报,2009,10(2)：195-200.图2 96份芫荽品种的Nei’s遗传距离矩阵聚类图Fig-2 Dendrogram of cluster analysis for 96 coriander germplasm based on Nei’s distance［11］Bendhifi M,Baraket G,Zourgui L,et al.Assessment of genetic diversity of Tunisian Barbary fig (Opuntia ficus indica) cultivars by RAPD markers and morphological traits［J］.Scientia Horticulturae,2013,158：1-7.
［12］侯茜,雷英,刘丽莎,等.西部地区濒危药用植物秦艽遗传多样性的RAPD分析［J］.中华中医药杂志,2013,28(1):214-216.

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备注/Memo

第一作者简介：李小梅（1982-），女，硕士研究生，农艺师，现主要从事辣椒及芫荽栽培与育种等研究工作。E-mail:eileen828@126.com.

责任作者：张景涛（1963-），男，硕士，研究员，现主要从事蔬菜育种等研究工作。E-mail:chiillii@126.com.

更新日期/Last Update: 2014-09-21