[1]方子义,赵世萍,宋晓惜,等.氯仿法提取蜡梅花被片表层蜡质的研究[J].北方园艺,2013,37(23):96-99.
　FANG Zi-yi,ZHAO Shi-ping,SONG Xiao-xi,et al.Study on the Extraction of Waxy from the Tepals Surface of Chimonanthus by Chloroforme[J].Northern Horticulture,2013,37(23):96-99.
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氯仿法提取蜡梅花被片表层蜡质的研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年23期 页码: 96-99 栏目: 出版日期: 2013-12-15
Title:
Study on the Extraction of Waxy from the Tepals Surface of Chimonanthus by Chloroforme
文章编号:
1001-0009（2013）23-0096-04
作者:
方子义; 赵世萍; 宋晓惜; 王倩丽; 赵凯歌; 陈龙清
华中农业大学，园艺植物生物学教育部重点实验室，湖北 武汉 430070
Author(s):
FANG Zi-yi；ZHAO Shi-ping；SONG Xiao-xi；WANG Qian-li；ZHAO Kai-ge；CHEN Long-qing
Key Laboratory of Horticultural Plant Biology of Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan, Hubei 430070
关键词:
氯仿法; 蜡梅; 花被片; 蜡质含量
Keywords:
chloroform method; Chimonanthus nitens; petal; wax content
分类号:
Q 949.747.2
文献标志码:
A
摘要:
以山蜡梅、“金蓓”素心蜡梅及“扬州黄”素心蜡梅为试材，使用扫描电子显微镜观察，研究了氯仿加热不同时间处理对蜡梅盛开期花被片蜡质提取效果的影响。结果表明：山蜡梅花被片蜡质提取最适条件为氯仿60℃处理30 s，表层蜡质含量约为1 034.33 μg/cm2；素心蜡梅“金蓓”和“扬州黄”花被片蜡质提取最适条件均为氯仿60℃处理20 s，“金蓓”素心蜡梅花被片表层蜡质含量约为651.33 μg/cm2，“扬州黄”素心蜡梅花被片表层蜡质含量约为1 057.33 μg/cm2。
Abstract:
Taking Chimonanthus nitens Oliv,Chimonanthus praecox ‘Jinbei’ and Chimonanthus praecox ‘Yangzhouhuang’ as meterials,the treated samples were observed by scanning electron microscope （SEM）for establishing the method of the petal surface wax extraction during their full bloom period by using hot chloroform treatments.The results showed that the treatment with chloroform of 60℃ for 30 s was optimal for the petal surface wax extraction of C.nitens Oliv,and its petal surface wax content during the full bloom period was about 1 03433 μg/cm2.The treatment with chloroform of 60℃ for 20 s were optimal both for the samples of C.praecox ‘Jinbei’ and of C.praecox ‘Yangzhouhuang’.Their petal surface wax content during the full bloom period were about 651.33 μg/cm2 extracted from C.praecox (L.)Link and 1 057.33 μg/cm2?from C.praecox ‘Yangzhouhuang’.

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备注/Memo

第一作者简介：方子义(1987-)，男，硕士研究生,研究方向为园林植物种植资源创新及利用。E-mail：fangziyi163@126.com.

责任作者：赵凯歌（1975-），女，博士，副教授，中国花协荷花分会副秘书长,现主要从事我国特有园林植物和新优园林植物的种质资源调查收集及分子与形态评价和离体保存与利用分子育种和常规育种手段进行园林植物资源的种质创新等工作。E-mail:zhaokaige@mail.hzau.edu.cn.
基金项目：国家自然科学基金资助项目（31272207；30800762）。
收稿日期：2013-09-10

更新日期/Last Update: 2014-08-24